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1.
[目的]研究不同碳源和植物生长调节剂对亳芍愈伤组织生长和芍药苷积累的影响。[方法]以亳芍根茎为外植体,改变培养基中碳源(蔗糖、果糖、葡糖糖、麦芽糖、可溶性淀粉)和植物生长调节剂种类(6-BA、NAA、2,4-D),考察亳芍愈伤组织增长量和芍药苷积累量。[结果]碳源中,对亳芍愈伤组织的增长量以蔗糖最高,葡糖糖最有利于芍药苷积累;组合使用植物生长调节剂6-BA、NAA和2,4-D比单独使用6-BA更有利于愈伤组织生长和芍药苷积累,但高浓度的2,4-D抑制愈伤组织生长和芍药苷积累。[结论]确定了亳芍愈伤组织生长和芍药苷积累的最佳碳源和植物生长调节剂组合,为葡萄糖30 g/L+6-BA 0.5 mg/L+NAA 0.2 mg/L+2,4-D 0.2 mg/L。  相似文献   
2.
Jiang F  Zhao Y  Wang J  Wei S  Wei Z  Li R  Zhu Y  Sun Z  Xiao X 《Fitoterapia》2012,83(2):415-421
A comparative study was designed and conducted to compare the pharmacokinetic difference of paeoniflorin and albiflorin after oral administration of Radix Paeoniae Rubra to normal rats and the acute cholestasis hepatitis rats induced by alpha-naphthylisothiocyanate (ANIT). UPLC-ESI-MS/MS method was employed to determine the level of paeoniflorin and albiflorin in rat plasma using geniposide as the internal standard (IS). Unpaired Student's t-test was used for the statistical comparison. The investigation showed that there were significant differences between the normal rats and the acute cholestasis hepatitis rat groups in calculated parameters, such as AUC(0-t), AUC(0-∞), T(max) and CLz/F. The results indicated that acute liver injury in rats could alter the pharmacokinetics of drug. Since patients are the final users of the drug, it is essential to investigate the pharmacokinetics of the drug in disease status. Therefore, we used normal rats and the acute cholestasis hepatitis rats to study pharmacokinetics of Radix Paeoniae Rubra with the purpose of examining the influence of disease on the metabolic course.  相似文献   
3.
AIM: To investigate the therapeutic and preventive effects of paeoniflorin (PF) on APP/PS1 mice, and to explore the possible mechanism. METHODS: Fifteen male 5-month-old APP/PS1 non-dominant mice were chosen as normal control group, 15 male 5-month-old APP/PS1 double transgenic mice were used as model group, and 15 male 5-month-old APP/PS1 double transgenic mice treated with 5 mg/kg PF by intraperitoneal injection were allocated in administation group. The learning and memory ability of the mice in each group was detected by Morris water maze. The apoptosis was assessed by TUNEL fluorescence staining. The protein expression of PI3K, Akt, p-PI3K, p-Akt, caspase-3, caspase-9, Bcl-2 and Bax in cerebral cortex and hippocampus was detected by Western Blot. The protein expression levels and distribution of caspase-3 and caspase-9 were detected by immunohistochemistry. RESULTS: (1) Compared with normal control group, the learning and memory ability declined in APP/PS1 model group. Compared with APP/PS1 model group, PF obviously improve the ability of learning and memory in mice. (2) Compared with normal control group, the apoptosis of nerve cells in APP/PS1 model group significantly increased and distributed in wider areas, while that in PF group was reduced (P<0.05). (3) Compared with APP/PS1 model group, PF could significantly lower pro-apoptotic factors, caspase-3, caspase-9 and Bax (P<0.05), and increase the expression of anti-apoptotic factors, p-PI3K, p-Akt and Bcl-2 (P<0.05). CONCLUSION: PF can up-regulate the expression of Bcl-2 and down-regulate the expression levels of caspase-9, caspase-3 and Bax via the activation of PI3K/Akt pathway, thereby inhibiting the nerve cell apoptosis and protecting the nerve cells, so as to treat neurodegenerative diseases.  相似文献   
4.
AIM: To investigate the mechanisms by which paeoniflorin (Pae) attenuates lipopolysaccharide(LPS)-induced acute lung injury in mice. METHODS: Male BALB/c mice were randomly divided into 4 groups: control,LPS, Pae+LPS, and Pae. Mice were administered intragastrically with double distilled water or Pae (20 mg/kg) once a day for 3 days. One hour after intragastrical treatment on the third day, LPS (20 mg/kg) or normal saline was injected intraperitoneally. Twelve hours after LPS challenge, the histological changes of the lung were observed, and histology score was also assessed. The myeloperoxidase (MPO),cytosolic phospholipase A2 (cPLA2) and phosphorylated cytosolic phospholipase A2 (phospho-cPLA2) in lung tissues were detected by Western blotting.RESULTS: LPS challenge resulted in acute lung injury, activated cPLA2 and increased MPO content in lung. Pretreatment with paeoniflorin significantly attenuated lung injury induced by intraperitoneal injection of LPS. The levels of MPO and phospho-cPLA2 in the lung tissues of the mice in Pae+LPS group were lower than those in LPS group (P<0.05).CONCLUSION: Pretreatment with paeoniflorin remarkably reduces LPS-induced acute lung injury through inhibiting phosphorylation of cPLA2 and decreasing neutrophil infiltration in the lung. These findings provide a new strategy for the prevention and treatment of LPS-induced acute lung injury.  相似文献   
5.
6.
AIM: To investigate the effects of paeoniflorin (Pae) on the functional responses induced by acute hypoxic insult in the rat cerebellar Purkinje cells (PCs).METHODS: The whole-cell patch clamp was used for the intracellular recording of PCs in the rat cerebellar slices to evaluate the changes of membrane potential, the excitability of PCs, and the parallel fibre (PF)-PC excitatory postsynaptic currents (EPSCs) upon acute hypoxic insult alone or with the pre-sence of Pae.RESULTS: PCs showed an initial hyperpolarization followed by brief depolarization and long lasting post-hypoxia hyperpolarization after hypoxia exposure. Pae completely blocked hypoxia-induced hyperpolarization and decreased the amplitude and the duration of hypoxic depolarization. Hypoxia up-regulated the excitability of rat PCs. Pae didn't show any significant effect on the hypoxia-induced hyperexcitability in PCs. Acute hypoxia induced long-term depression (LTD) in rat cerebellar PF-PC EPSCs, and Pae partially reversed hypoxia-induced depression in PF-PC EPSCs.CONCLUSION: Pae significantly suppresses hypoxia-induced responses in rat PCs and probably increases the tolerance of rat PCs to acute hypoxia.  相似文献   
7.
采用HPLC法测定醒脑抗栓胶囊中芍药甙的含量。色谱条件:YWG-C18柱,2.6 mm×250 mm,10μm;流动相:乙腈-磷酸盐缓冲液(0.05 mol/LNa H_2PO_4,用Na OH调p H6.0)(20∶80),流速1.2 ml/min;量程0.02AUFS;检测波长230 nm。测定结果,平均回收率为94.60%,RSD=2.70%。  相似文献   
8.
[目的]建立同时测定六味地黄胶囊中马钱苷、芍药苷和丹皮酚3种成分含量的反相高效液相色谱法(RP-HPLC)。[方法]以Waters ODS C18(4.6 mm×250 mm,5μm)柱为分析柱、甲醇-0.1%磷酸为流动相进行梯度洗脱,流速为1.0 ml/min,检测波长芍药苷和马钱苷为230 nm、丹皮酚为274 nm,柱温40℃。[结果]芍药苷在0.066 5~1.663 2μg、马钱苷在0.107 9~2.697 7μg、丹皮酚在0.122 4~3.060 0μg范围内均具有良好的线性关系,相关系数(r)分别为0.999 4、0.999 8、0.999 9,平均回收率为芍药苷97.63%(RSD=0.82%)、马钱苷97.66%(RSD=0.74%)、丹皮酚96.22%(RSD=1.21%)。[结论]该方法简便、准确、重复性好,可用于六味地黄胶囊的质量控制。  相似文献   
9.
牡丹种皮中芍药苷和丹皮酚的优化提取工艺   总被引:1,自引:0,他引:1  
[目的]采用乙醇匀浆法优选牡丹种皮中芍药苷和丹皮酚的最佳提取工艺。[方法]在单因素试验基础上采用L9(34)正交试验设计,考察了乙醇浓度、料液比、匀浆时间、提取次数对芍药苷和丹皮酚提取得率的影响,以确定最佳提取工艺参数条件。[结果]最佳工艺条件为乙醇浓度60%、料液比1∶20、匀浆时间4 min、提取1次。[结论]采用乙醇匀浆正交试验法优化的提取工艺可以有效提高牡丹种皮中芍药苷和丹皮酚得率。  相似文献   
10.
谭家华  罗君  贺祝英  张建玲  于佳 《安徽农业科学》2012,(12):7067-7068,7071
[目的]建立眼保Ⅰ号胶囊中葛根素和芍药苷的含量测定方法。[方法]色谱柱为Diamonsil C18(2)(4.6 mm×250 mm,5μm);流速为1 ml/min;柱温为25℃;葛根素流动相为无水甲醇-水(28∶72,V/V),检测波长为250 nm;芍药苷流动相为乙腈-浓度0.1%磷酸溶液(14∶86,V/V),检测波长为230 nm。[结果]葛根素在0.001 18~0.007 08μg/ml浓度范围内浓度与峰面积的线性关系良好(r=0.999 9),平均回收率102.4%,RSD为1.17%(n=6);芍药苷在0.044~0.261μg/ml浓度范围内浓度与峰面积的线性关系良好(r=0.999 9),平均回收率97.8%,RSD为1.71%(n=6);眼保Ⅰ号胶囊内容物,葛根素和芍药苷的平均含量分别为0.055和0.168 mg/g。[结论]该方法简单,准确,重现性好,可用于眼保Ⅰ号胶囊中葛根素和芍药苷的含量检测。  相似文献   
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