Chemical composition and anti-Candida potencial of the extracts of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae)

Phytochemical prospecting was performed by HPLC-DAD. The Inhibitory Concentration of 50% of mortality the microorganisms (IC₅₀) was determined and a cell viability curve was obtained. Minimum Fungicidal Concentration (MFC) was determined by subculture in Sabourad Dextrose Agar. The effect of the combination extract/fluconazole was verified by microdilution, with the extracts in subinhibitory concentrations (MFC/16). Caffeic acid was the major compound of both extracts, representing 6.08% in the aqueous extract and 7.62% in the ethanolic extract. The extracts showed a fungistatic effect (MFC ≥ 16,384 μg/mL). The IC₅₀ results demonstrated that the combination of the extracts with fluconazole were more significant than the products tested alone, with values from 4.9 to 34.8 μg/mL for the ethanolic extract/fluconazole and 5 to 84.7 μg/mL for the aqueous extract/fluconazole. The potentiating effect of fluconazole action was observed against C. albicans and C. tropicalis. In C. krusei the aqueous extract had an antagonistic effect.     

盐酸特比萘芬对家蚕真菌病的防治效果试验初报

以家蚕真菌病病原为受试菌,对抗真菌药物氟康唑(FCZ)和盐酸特比萘芬(TBF·HCl)进行了体外筛选,研究了盐酸特比萘芬对家蚕真菌病的防治效果及其稳定性。试验结果表明,盐酸特比萘芬的体外抑菌、杀菌效果优于氟康唑,盐酸特比萘芬对家蚕病原真菌的最小抑菌浓度范围为0.0625～0.2726μg/mL,最小杀菌浓度范围为0.125～4μg/mL。生物试验表明,盐酸特比萘芬对家蚕真菌病防治效果良好,200μg/mL时防治效果达100%,且对家蚕生长发育、茧质无不良影响。稳定性试验表明盐酸特比萘芬长期稳定性良好。     

Fluconazole decreases cyclosporine dosage in renal transplanted dogs

The effect of fluconazole (Fcz) on the cyclosporine (CsA) dosage was investigated in renal transplanted dogs receiving CsA-based immunosuppressive therapy. Initially, CsA was administered orally twice daily to raise the blood trough level between 400 and 600 ng/ml. After the addition of Fcz, the CsA dosage was adjusted to maintain its therapeutic blood concentration. Fcz significantly decreased CsA dosage in both normal and renal transplanted dogs, but a higher dosage of CsA was needed in renal transplanted dogs. In conclusion, Fcz decreases required CsA dosage and thereby reduces the cost of immunosuppressive therapy in canine renal transplantation.     

细胞培养过程中白色念珠茵污染的防控

为探讨大扶康防控细胞培养过程中白色念球菌污染的可行性,采用不同浓度的大扶康共同培养,观察其抑制白色念珠菌的效果,及对小鼠成肌细胞C2C12生长情况的影响。结果:采用300μg/ml大扶康防控效果最佳,毒性作用小,对C2C12细胞的生存率无明显影响。采用30μg/ml大扶康与20μg/ml硫酸阿米卡星 50μg/ml头孢他啶配伍的方法可有效预防细胞培养过程中的真菌污染。结论:采用大扶康可有效控制细胞培养中白色念珠菌的污染。采用大扶康与硫酸阿米卡星 头孢他啶配伍的方法可有效预防细胞培养过程中的真菌污染。     

Equine Endometrial Tissue Concentration of Fluconazole Following Oral Administration

The objective of this study was to determine the plasma and endometrial tissue concentrations of orally administered fluconazole and to determine if these tissue levels surpassed the minimum inhibitory concentration (MIC) for Candida spp. organisms in the reproductive tract of the mare. Mares from study 1 (n = 9) were administered a single oral loading dose of 14 mg/kg fluconazole. Plasma and endometrial tissue samples were collected before fluconazole administration and for 24 hours after the loading dose. Study 2 mares (n = 3), a subset of study 1, were administered the loading dose, followed by maintenance doses of 5 mg/kg every 24 hours for 6 days. Plasma and biopsy samples were collected for 48 hours after the last maintenance dose. High pressure liquid chromatography-tandem mass spectrometry was used to determine the concentration of fluconazole in all samples. The mean plasma and endometrial fluconazole levels 24 hours after the loading dose were 9.53 ± 0.824 μg/mL (mean ± standard deviation) and 11.3 ± 2.38 μg/g, respectively. Fluconazole levels in plasma and endometrial tissue 24 hours after the last maintenance dose were 7.82 ± 1.81 μg/mL and 7.23 ± 3.86 μg/g, respectively. Oral fluconazole administered as a 14-mg/kg loading dose and a 5-mg/kg maintenance dose every 24 hours will result in endometrial tissue levels near the accepted MIC values for most Candida spp. and surpass the MIC for Candida albicans in the reproductive tract of the mare. Consequently, this dosage regimen could be considered for the treatment of infectious endometritis caused by susceptible fungal organisms of Candida spp. in the mare.     

细胞培养过程中白色念珠菌污染的防控

为探讨大扶康防控细胞培养过程中白色念球菌污染的可行性,采用不同浓度的大扶康共同培养,观察其抑制白色念珠菌的效果,及对小鼠成肌细胞C2C12生长情况的影响。结果：采用300 μg/ml大扶康防控效果最佳,毒性作用小,对C2C12细胞的生存率无明显影响。采用30 μg/ml大扶康与20 μg/ml硫酸阿米卡星＋50 μg/ml头孢他啶配伍的方法可有效预防细胞培养过程中的真菌污染。结论：采用大扶康可有效控制细胞培养中白色念珠菌的污染。采用大扶康与硫酸阿米卡星＋头孢他啶配伍的方法可有效预防细胞培养过程中的真菌污染。