甘薯AFLP扩增产物的PAGE胶快速银染方法

对目前6种常用的PAGE银染方法的成本、时间及染色效果等进行比较.结合笔者所在实验室的具体情况,用简易强碱法对甘薯AFLP扩增产物进行银染,分析银染过程中漂洗时间、次数,银染用水对银染效果的影响,建立了甘薯AFLP扩增产物的PAGE胶快速银染方法,可以批量生产银染AFLP扩增产物,银染效果较好.     

不同葡萄品种膜质过氧化和保护酶活性对盐胁迫的响应

研究了不同浓度NaCl处理对双丰、左山二、贝达以及金玫瑰细胞膜透性、膜质过氧化和保护酶活性的影响。结果表明:不同处理间,4个品种耐NaCl胁迫的动态响应表现出显著差异。随胁迫浓度的增大和胁迫时间的延长,MDA含量和细胞膜透性表现出增高趋势,增高幅度由高到低的顺序为双丰、左山二、贝达、金玫瑰;保护酶SOD活性整体表现出先增后降趋势,金玫瑰和贝达升高幅度相对较大;POD活性整体表现出下降趋势,但金玫瑰和贝达下降幅度相对较小。     

Salt Stress Effects on Germination,Plant Growth and Accumulation of Metabolites in Five Leguminous Plants

The investigation was conducted to determine physiological criteria of early selection for salt tolerant leguminous plants. Plants were subjected to 5 levels of salt stress at the roots （0, 50, 100,150 and 200 mM NaCI）. Results showed that sodium chloride had an underrating effect on growth of stems and seed germination of the species studied. The germination rates of seeds of Glycine max and Phaseolus vulgaris （sensitive glyeophytes） were affected from 3 g/L of NaCl, with critical thresholds at 9 and 12 g/L respectively. In contrast, critical thresholds with Mucunapoggei （facultative halophyte）, Vigna unguiculata （moderately tolerant glycophyte） and P. adenanthus （natural halophyte） was found to be above 21 g/L. The reduction of stems growth rate were not significant in P. adenanthus whereas in M. poggei and V. unguiculata this inhibition was observed just when nutritive solutions were enriched with 200 mM. The lipid contents were reduced in all the species under salt stress, whereas proteins and proline contents in the leaves were substantially increased in tolerant species （M. poggei, P. adenanthus and V. unguiculata）. In contrast, proteins and leaf proline contents were negatively affected by salt concentration to G. max and P. vulgaris. Seed germination, proteins and proline could be used as physiological criteria of early selection for salt tolerant leguminous plants.     

Antioxidant Activity of Pigment Extracted from Green-Wheat-Bran

The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity.The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation,total antioxidant activity （TAA）,superoxide anion radical scavenging activity （SARSA）,active oxygen scavenging activity （AOSA）,and DPPH （1,1-diphenyl-2 picrylhydrazyl free radical） radical scavenging activity.The results showed that the pigment had higher antioxidant activity and TAA,SARSA,AOSA and DPPH.scavenging activities at a certain concentration than Vc （antiscorbutic vitamin,vitamin C）,and the capacity increased with the increase of pigment concentration.Its TAA was 51.06 U mL-1,1.73 times of Vc,and SARSA 18 025.21 U mL-1,2.26% higher than Vc,and AOSA 3 776.31 U mL-1,1.24 times of Vc.As to the DPPH.scavenging activity of the pigment,there was a trend that higher concentration performed higher activity significantly improved with the company of Vc.The pigment showed significant antioxidant activities evaluated by different assays.Results will provide a better understanding on antioxidant activity of green wheat and allow the screening or breeding of green wheat varieties with higher antioxidant activity for food processing.     

中西结合治疗犬慢性肾功能衰竭

犬慢性肾功能衰竭(chronic renal failure,CRF)是临床常见病之一,是一种不可逆的进行性病变,尤其是老龄犬多发。西医称慢性肾功能衰竭,是指各种慢性肾脏疾病或全身性疾病累及肾脏,使肾脏受损,肾脏参与和调节的排泄和内分泌功能减退,而致体内代谢产物潴留,水、电解质及酸碱平     

科普知识

什么是转基因?转基因技术可用来改变植物的某些遗传特性,培育高产、优质、抗病毒、抗虫、抗寒、抗旱、抗涝、抗盐碱、抗除草剂等的作物新品种;可用转基因植物或离体培养的细胞来生产外源基因的表达产物,如人的生长素、胰岛素、干扰素、白介素、表皮生长因子、乙型肝炎疫苗等基因已     

机构简介 湖南农业大学茶学教育部重点实验室 湖南省天然产物工程技术研究中心 湖南农业大学天然产物研究中心

<正>湖南农业大学茶学重点实验室前身为湖南农业大学天然产物研究中心和湖南农业大学茶叶研究所。2003年7月经湖南省科技厅批准正式组建湖南省天然产物工程技术研究中心,2005年验收并获得A类工程技术研究中心称号。2003年11月被教育部批准为省部共建茶学重点实验室,2006年通过验收并授牌。2007年3月湖南省科技厅批     

微生物制剂产品在养殖业的应用及其误区

1应用的意义进入21世纪,伴随生物技术的迅猛发展,消费结构和理念的变化,使得养殖业以高效、安全、健康和环保为发展方向。但养殖业发展始终面临营养、保健、预防问题。现代畜牧业的发展,全价饲料不仅提     

猪硝酸盐与亚硝酸盐中毒防治

硝酸盐与亚硝酸盐中毒是猪摄入堆积发酵而含有硝酸盐或亚硝酸盐的青绿食物引起的,以皮肤粘膜发绀、呼吸困难为主要症状的一种中毒病。临床上以夏、秋季食欲旺盛的架子猪频发多见,致死率较高是因为发现与治疗不及时所致。1病因亚硝酸盐是硝酸盐在硝酸菌的作用下还原为氨过程的中间产物;它的产生取决于硝酸盐的数量与硝化菌的活跃程     

中草药饲料添加剂对河西肉牛血液生化指标影响的研究

为了提升河西肉牛产品特别是血液制品的质量及产业效益,利用饲料中添加中草药添加剂的方法对河西肉牛架子牛进行了育肥、屠宰试验。采取低剂量中草药组、高剂量中草药组和对照组肉牛血样,用专门仪器和方法测定了血液中5种血清矿物质离子、7种血清代谢产物及5种血清酶等生化指标的含量。结果表明:高剂量中草药组肉牛血清中的Na、Mg离子及乳酸脱氢酶含量均极显著高于低剂量组和对照组(P<0.01),Cl离子含量极显著高于对照组(P<0.01),其葡萄糖、肌酐、甘油三酯含量均显著高于对照组(P<0.05),谷丙转氨酶显著低于其他两组肉牛(P<0.05),而谷草转氨酶(AST)却显著高于其他两组肉牛(P<0.05);低剂量组和高剂量组肉牛血清中的高密度脂蛋白极显著高于对照组(P<0.01),碱性磷酸酶极显著低于对照组肉牛(P<0.01),而谷丙/谷酸则显著高于对照组肉牛(P<0.05)。结论:试验配制的中草药饲料添加剂对肉牛血液生化指标有良好的影响,对高剂量组肉牛的影响更为明显;饲喂中草药饲料添加剂的肉牛生长发育、营养状况均显著好于对照组,但高剂量组更优;利用高剂量中草药饲料添加剂饲喂的肉牛,其血液在制造保健用品方面有广阔开发前景。