Organic Matter Added to Bareroot Nursery Beds Influences Soil Properties and Morphology of Fraxinus pennsylvanica and Quercus rubra Seedlings

Bareroot hardwood seedling production involves intensive soil management. To increase soil organic matter (OM), nurseries commonly grow a cover crop for 1 year after every 1–2 year of seedling production. Raising soil OM levels can also be achieved through addition of soil amendments. We studied the influence of chicken manure (CM) and composted leaf, tree, and lawn trimmings (Cp) on soil properties and morphology of green ash (Fraxinus pennsylvanica Marsh.) and northern red oak (Quercus rubra L.) seedlings. CM was applied at 725, 1450, or 2900 kg ha⁻¹ (CM725, CM1450, and CM2900, respectively) and Cp was applied at 200 m³ ha⁻¹. Addition of CM and Cp significantly raised soil OM levels and altered soil chemical properties compared to the control (Ctrl). Root-collar diameter increased with addition of CM1450, CM2900, or Cp compared to CM725 or Ctrl plots for northern red oak, but was largest in soils amended with CM2900 for green ash. Conversely, height was greatest with addition of CM725 for northern red oak, but green ash seedlings were shorter in Ctrl plots than in all amendments except for CM725. Root volume of green ash and northern red oak seedlings was positively influenced by addition of CM or Cp. Seedling responses to nursery soil amendments vary with different forms and amounts of OM. Benefits to seedling growth through application of appropriate materials in the proper balance can improve seedling morphological quality and positively influence soil chemical properties.     

断母乳日龄对犊牛营养物质消化和血清生化指标的影响

本试验旨在研究不同断母乳日龄对犊牛营养物质消化和血清生化指标影响。试验选用新生红安格斯与西门塔尔杂交肉犊牛60头,根据日龄和体重随机分成5个处理,每个处理12头犊牛,公母各占1/2,试验组分别在28、42、56和70日龄进行断母乳(分别为28A、42B、56C、70D组),随后人工哺乳代乳品至90日龄;对照组( CK组)随母哺乳至150日龄。试验时间为出生至150日龄,期间测定体重(0、70、90、150日龄)和血清生化指标(70、90、150日龄)的变化。每个处理选取6头犊牛分别在断奶前(85~90日龄)和断奶后(145~150日龄)进行2期消化代谢试验。每期6 d,其中2 d预试期,4 d正试期。结果显示：1)试验全期体重各组间无显著差异(P>0.05)。2)断奶前各组犊牛营养物质消化率差异不显著(P>0.05),断奶后28A、42B组犊牛干物质消化率显著高于56C、70D组( P<0.05),有机物消化率显著高于70D组( P<0.05),其中42B组消化率略高。3)150日龄试验结束时,28A和42B组血清葡萄糖、总蛋白、甘油三酯、尿素氮、胆固醇、结合珠蛋白、白细胞介素-2、胰岛素样生长因子-1、免疫球蛋白M含量与CK组相比差异不显著( P>0.05),血清免疫球蛋白G、免疫球蛋白A含量分别显著低于、高于CK组( P<0.05),其中42B组略高。由此可见,红安格斯与西门塔尔杂交肉犊牛的断母乳时间以42日龄较为适宜。     

Phenotypic and genotypic characterization of canine pyoderma isolates of Staphylococcus pseudintermedius for biofilm formation

Biofilm-forming ability is increasingly being recognized as an important virulence factor in several Staphylococcus species. This study evaluated the biofilm-forming ability of sixty canine derived clinical isolates of S. pseudintermedius, using three phenotypic methods, microtiter plate test (MtP), Congo red agar method (CRA) and tube adherence test, and the presence and impact of biofilm-associated genes (icaA and icaD). The results showed that icaA and icaD genes were detected concomitantly in 55 (91.7%) of 60 isolates. A majority (88.3%) of the strains screened had matching results by the tube adherence test, MtP and PCR analysis. Better agreement (95%) was found between the PCR-based analysis and the CRA. Results of the icaA and icaD gene PCRs showed good agreement with CRA results, with a kappa of 0.7. Comparing the phenotypic methods, the statistical analysis showed that the agreement among the phenotypical tests using categorical data was generally good. Considering two classes (biofilm producer and biofilm non-producer), the percentage of matching results between the CRA method and the tube adherence test and between the CRA method and the MtP was 93.3%. A concordance of 100% was revealed between the MtP and the tube adherence test. The results indicate a high prevalence of the ica genes within S. pseudintermedius isolates, and their presence is associated with in vitro formation of a biofilm. A combination of phenotypic and genotypic tests is recommended for investigating biofilm formation in S. pseudintermedius.     

Investigation of biofilm production and icaA and icaD genes in Staphylococcus aureus isolated from heifers and cows with mastitis

Biofilm formation and antimicrobial resistance of Staphylococcus aureus are important virulence factors in cases of mastitis in dairy cows. However, few studies have investigated mastitis strains isolated from heifers. Within this context, the objective of the present study was to investigate biofilm formation on Congo red agar, the presence of the icaA and icaD genes by polymerase chain reaction (PCR), and the percentage of in vitro antimicrobial resistance of 110 S. aureus isolates from mammary gland secretions of heifers and cows with mastitis. PCR detected the icaA and icaD genes in 98% and 100% of isolates, respectively. However, only 55.5% of all isolates produced a biofilm on Congo red agar. Antimicrobial susceptibility testing revealed that 47.0% of isolates from heifers and 70.4% of isolates from cows were resistant to at least one of the antimicrobial agents tested. Resistance to penicillin and/or ampicillin was the most frequent (44.5%). These results indicate the need to implement prophylactic and control measures of mastitis for heifers. Heifers and cows can carry resistant strains with the capacity of biofilm production, a fact representing a threat to public health and animal well‐being and generating losses to dairy farmers.     

外源多酚添加条件下干红葡萄酒颜色和花色苷特性研究

颜色是红葡萄酒重要的感官特征和质量评价指标,花色苷是红葡萄酒呈色、稳定和营养功能的重要物质基础。既往研究极少探讨外源添加天然色素对红葡萄酒颜色质量和花色苷的影响。选取赤霞珠干红葡萄酒为研究对象,于酒精发酵前和发酵后分别添加天然色素Ⅰ和天然色素Ⅱ2种色素,并以添加黄岑苷、绿原酸和没食子酸3种多酚为对照,探究对葡萄酒颜色特征、花色苷种类与含量的影响。结果发现：在发酵前添加天然色素Ⅰ和Ⅱ使得酒体颜色加深、红色色调加强、花色苷含量增加。在发酵后添加以上多酚对葡萄酒颜色和花色苷的影响弱于发酵前添加,但添加天然色素Ⅰ仍然有利于花色苷含量的保持,只是效果弱于发酵前添加。其余多酚物质处理对供试酒样颜色品质影响较小。研究结果表明发酵前添加多酚物质更有利于红葡萄酒颜色品质的提升和稳定、花色苷含量的保持和稳定,天然色素Ⅰ是相对最优质的辅色剂。     

不同红蓝LED光照时间对冰菜生长和品质的影响

【目的】 研究不同红蓝光照时间对冰菜生长发育的影响,分析其生长发育规律。【方法】 以冰菜为研究对象,以红蓝光为人工光源,在植物工厂及水培条件下,采用可循环营养液(EC2.8 dS/m,pH6.5),待冰菜幼苗长至4片叶后移植到水培立体培养系统中,设置红光与蓝光比例为(3∶1),光强统一设定为300 μmol/(m²·s),补光时间设定为8 h/16 h(光/暗)为A处理,10 h/14 h(光/暗)为B处理,12 h/12 h(光/暗)为C处理,14 h/10 h(光/暗)为D处理,16 h/8 h(光/暗)为E处理。【结果】 随着光照时间的增加冰菜叶片净光合速率也增大,冰菜在14 h光照处理下,生长形态最优,光合色素含量最高,在14 h光照下,净光合速率达到最大,但达到16 h时,净光合速率开始降低,且Fv/Fm值和Φ值最小;在14 h光照处理下抗氧化酶活性较高,在12 h光照处理下有利于可溶性糖以及VC的积累,随着光照时间的延长达到16 h时,冰菜叶片中硝酸盐含量最高。【结论】 冰菜在红蓝光比例为3∶1时,光照时间为14 h能更有利于冰菜的生长以及改善其营养品质。     

不同光强的LED白光与红蓝光对生菜生长及营养元素含量的影响

在人工光植物工厂中研究了不同光强的LED白光和红蓝光对营养液水培生菜生长、营养元素含量和累积量的影响。试验设置白光（WL）和红蓝光（RB）两个光质处理,光周期为16/8 h,分别设定三个光强：150（WL150、RB150）、200（WL200、RB200）和250（WL250、RB250）μmol·m^-2·s^-1,其中红蓝光光强比为4∶1,光暗周期16/8 h。结果表明,光强增加有促进生菜生长作用,随光强增加生菜地上部干、鲜重明显增加,WL200和WL250处理下生菜地上部干重比WL150分别提高了24.6%和49.7%。与红蓝光相比,白光显著提高生菜叶面积和地上部生物量。红蓝光提高了生菜叶片中大中量元素（N、P、Ca、Mg）和微量元素（Mn、Zn）的含量,白光则提高了K的含量,光质对C、Fe和Cu含量无明显影响。白光或红蓝光下不同光强对生菜叶片营养元素含量的影响并没有表现出明显的规律,只是红蓝光在200 μmol·m^-2·s^-1光强下多种营养元素（N、P、Ca、Mg、Mn、Cu和Zn）含量到达最大。在白光200 μmol·m^-2·s^-1的光强下,P、K、Ca、Mg、Fe、Mn、Cu和Zn的累积量最大,而在红蓝光250 μmol·m^-2·s^-1的光强下,10种营养元素的累积量均最大。相同光强下,红蓝光有利于生菜体内N、P、Ca、Mg、Mn和Zn含量的提高,而白光则更有利于C、N、P、K、Mg和Fe等的累积。因此白光处理更有利于促进生菜生长,并提高生菜体内N、C、P、K、Mg和Fe的累积量,其累积量的提高主要是由较高的生物量决定的。     

Effects of adrenaline on ionic equilibria in red blood cells of rainbow trout (Salmo gairdneri)

Effects of adrenaline on the equilibrium distributions of Na⁺ , K⁺ , H⁺ , Cl^– , and H₂O across the cell membrane of rainbow trout (Salmo gairdneri) erythrocytes were determinedin vitro, as a function of P CO₂ (1.76–7.77 torr). CO₂-carrying capacity of the blood was also examined. Plasma catecholamine concentrations inunanaesthetized, unrestrained trout were 3.1 nM adrenaline and 1.2 nM noradrenaline. Elevation of the plasma adrenaline concentrationin vitro to 4.6 × 10³ nM resulted in net gains of Na⁺ , Cl^– and H₂O by red cells, a net loss of H⁺ from red cells, and a pronounced red cell swelling. Adrenaline also reduced the CO₂-carrying capacity of trout bloodin vitro. The magnitudes of these effects increased with P_CO2 and, thus, were sensitive to blood HCO₃ ^– concentrations. The distribution of K⁺ between red cells and plasma was unaffected by adrenaline. Adrenergic-mediated ion movements and red cell swelling were sensitive to both propranolol and SITS. These results are consistent with the symport NaCl uptake model for adrenergic-mediated swelling of Baroinet al. (1984). The adrenergic response of fish erythrocytes may function to ameliorate the effects of blood acidoses on O₂-carrying capacity by maintaining red cell pH in the face of a decrease in plasma pH.     

赤潮对羊栖菜产量构成的影响

20 0 0年 4～ 6月 ,浙江省洞头海区发生了 2次赤潮。经测定 ,在该区栽培的羊栖菜 (Hizikiafusiforme)的鲜干比增加 ,日平均增长率、平均长度减少 ,营养生长减慢。羊栖菜个体的繁殖部分受影响较小 ,假根次之 ,主茎、次生叶 (气囊 )、分枝部分受影响最大 ,后者在羊栖菜产量中的比重最大。但在赤潮发生区的羊栖菜未出现明显病烂现象 ,生长发育正常 ,表明羊栖菜对赤潮有一定忍耐力