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81.
气相色谱-质谱法检测鱼肉中MS-222残留 总被引:1,自引:0,他引:1
本文建立了鱼肉中MS-222残留GC-MS检测方法.鱼肉样品经乙腈提取,氮吹浓缩,盐酸溶液引导MS-222电离,Waters Oasis MCX固相萃取柱净化后,气相色谱-四极杆质谱检测.方法检出限为2.5 μg·kg-1、定量限为5.0 μg·kg-1;0.0025~1.0 μg·mL-1范围内线性关系良好(R≥0.9996);MS-222浓度范围在5.0~ 100.0 μg·kg-1的鱼肉加标样,日内和日间平均回收率为78.4%~91.2%,相对标准偏差为3.62% ~9.49%.结果表明,该检测方法适用于低浓度水平鱼肉中MS-222残留检测. 相似文献
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microRNAs(miRNAs)在各种类型细胞增殖、分化和凋亡中发挥了重要作用。miR-138参与乳腺发育周期过程中细胞增殖分化的调控。试验以小鼠为动物模型,尾静脉注射miR-138基因抑制剂,应用幼鼠体重称重法,检测miR-138抑制后乳腺泌乳量变化;应用电子显微镜等技术观测乳腺组织形态变化,抑制miR-138后,小鼠乳腺上皮细胞增加,乳汁分泌量增加;抑制miR-138后,观察小鼠乳腺组织超微结构可发现,乳腺细胞的代谢活动增强;收集尾静脉注射miR-138 抑制剂的小鼠乳汁,检测发现其乳糖、乳中酪蛋白含量均有所增高。研究认为,miR-138可刺激乳腺上皮细胞增殖,增加乳腺发育泌乳过程中乳的分泌,并且调控乳汁中重要成分含量。 相似文献
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Bo WENG Mao-liang RAN Rong CAO Fu-zhi PENG Hui LUO Hu GAO Xiang-wei TANG Anqi YANG Bin CHEN 《农业科学学报》2019,18(8):1924-1935
MicroRNAs (miRNAs) have been widely identified in porcine testicular tissues and implicated as crucial regulators of proliferation, apoptosis, and differentiation in porcine spermatogenesis related cells. However, the function roles of most of the miRNAs that have been identified in Sertoli cells are poorly understood. In the present study, six experiments were conducted to study the regulatory role of miR-10b in porcine immature Sertoli cells. In experiment 1, the results showed that the relative mRNA expression level of miR-10b in porcine testicular tissues decreased quadratically (P<0.001) with increasing age, while the relative mRNA expression level of DAZAP1 gene increased (P<0.001). In addition, the mRNA expression of miR-10b was negatively (P<0.01) correlated with DAZAP1 mRNA expression (r=?0.550). In experiment 2, the results from the bioinformatic analysis and a luciferase reporter assay demonstrated that miR-10b directly targeted the DAZAP1 gene in porcine immature Sertoli cells. DAZAP1 mRNA and protein expressions were both regulated (P<0.05) by miR-10b. In experiments 3 to 5, the over-expression of miR-10b or the siRNA-mediated knockdown of the DAZAP1 gene promoted (P<0.05) porcine immature Sertoli cell proliferation, as determined by the Cell Counting Kit-8 (CCK-8) assay and the 5-Ethynyl-2'-deoxyuridine (EdU) assay. However, an annexin V-FITC/PI staining assay and the expression of cell survival-related genes indicated that over-expression of miR-10b or knockdown of DAZAP1 had no effect (P>0.05) on porcine immature Sertoli cell apoptosis. In experiment 6, the co-transfection treatment results showed that miR-10b promoted (P<0.05) porcine immature Sertoli cell proliferation by targeting DAZAP1 gene. Overall, these experiments demonstrated that miR-10b promotes porcine immature Sertoli cell proliferation by targeting the DAZAP1 gene. 相似文献
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试验旨在构建锌指蛋白3(KLF3)基因3'-UTR区双荧光素酶基因报告载体及其突变载体,初步分析可能调控KLF3基因表达的miRNAs。首先通过PCR方法扩增KLF3基因的3'-UTR序列,将其克隆到经Xho Ⅰ、Not Ⅰ双酶切的双荧光素酶报告载体中;运用Targetscan软件预测可能与KLF3基因3'-UTR相互作用的miRNA;使用脂质体2000转染试剂将miRNAs mimics与构建好的KLF3基因3'-UTR段双荧光素酶报告载体或突变载体共转染于常规培养的293T细胞中,检测荧光素酶活性。结果表明,KLF3基因3'-UTR可能是miR-21的作用靶位点;双荧光报告显示,miR-21 mimics组(0.6900±0.0144)比突变组(1.000±0.0688)和空白对照组(1.000±0.0159)KLF3基因3'-UTR双荧光素酶基因报告载体和突变载体的活性降低了31%(P<0.01)。本试验成功构建了含有KLF3基因3'-UTR段双荧光素酶基因报告载体与突变载体,初步证实miR-21对KLF3基因有调控作用。 相似文献
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两种麻醉剂对罗非鱼的急性毒性及联合毒性研究 总被引:2,自引:0,他引:2
根据预试验结果确定最高安全浓度和最低全致死浓度,分别设置5个等对数浓度,进行MS-222和苯唑卡因及其1:1混合药物对罗非鱼(Oreochromis niloticus)的24h和96h毒性试验.结果:MS-222对罗非鱼的24h、96h半致死浓度及95%置信区间分别为88.84 mg/L(86.92~90.81 nag,/L)、76.18 mg/L(73.27~79.21 mg/L);苯唑卡因对罗非鱼的24h、96h半致死浓度及95%置信区间分别为44.49 mg/L(43.76~45.24 mg/L)、22.61 mg/L(20.29~25.20 mg/L);混合药物对罗非鱼的24h、96h半致死浓度及95%置信区间分别为63.35 mg/L(61.00~65.79 mg/L)、30.39 mg/L(29.48~31.34 mg/L).这表明:在同等剂量条件下,苯唑卡因对罗非鱼的毒性比MS-222强;将两种药物等比例混合后,在24h和96h毒性试验中,分别呈现出拮抗作用和协同作用. 相似文献
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The present study determined the effective concentrations of clove oil and MS‐222 in juvenile rohu Labeo rohita for quick induction and recovery. The immune‐biochemical responses due to 0, 1 and 24 hr exposure to those anaesthetics were also evaluated. Of four concentrations of the anaesthetics examined, the lowest effective concentration of clove oil and MS‐222 were 50 µl/L and 125 mg/L respectively. Clove oil and MS‐222 significantly increased the myeloperoxidase, total protein and alkaline phosphatase activity at some of the holding durations. However, superoxide anion production (after 0 and 1 hr) and antiprotease activity (after 24 hr) were significantly reduced in fish exposed to clove oil. Serum glucose content was significantly elevated in the MS‐222‐treated group. Furthermore, the clove oil‐treated group showed significantly higher levels of serum Na+ and K+, while the aspartate and alanine aminotransferase activities were significantly enhanced in the MS‐222 group. The use of both clove oil and MS‐222 is advised as an anaesthetic agent for rohu with a bias towards clove oil, considering its economic and operational feasibility. 相似文献