Molecular marker-based genetic linkage map of a diploid banana population (Musa acuminata Colla)

A well-saturated genetic linkage map is valuable for fundamental and applied genetic research. Genetic linkage maps of two half-sib diploid banana populations were constructed using allele-specific-polymerase chain reactions (AS-PCRs), diversity array technology (DArT), and simple sequence repeat (SSR) markers. Molecular maps were produced for each parent using the pseudo-testcross mapping strategy. The first maternal map (6142-1, 81 individuals) consisted of 231 markers divided as followed: 121 DArT, 106 SSR and 4 AS-PCR markers in 15 linkage groups (LGs) covering 670?cM. The second maternal map (6142-1-S, 58 individuals) contained a total of 152 markers including 71 DArTs, 79 SSRs, and 2 AS-PCRs mapped to 16 LGs that spanned 698?cM. The combined paternal map (139 individuals) comprised 316 markers (196 DArTs, 117 SSRs and 3 AS-PCRs) distributed over 15 LGs with a total map length of 1,004?cM. While distorted segregation of some markers was observed in all maps, this was much more frequent for the male parent. Homology between maps was assessed using common markers. While there was generally good congruity with regard to marker order across maps, incongruity in other cases may reflect chromosomal rearrangement events such as inversions, translocations, or deletions. The new banana map can provide a better understanding of the Musa genome and could be used for the identification of economically important traits and improvement of breeding strategies.     

The multielectron ionization dynamics underlying attosecond strong-field spectroscopies

Subcycle strong-field ionization (SFI) underlies many emerging spectroscopic probes of atomic or molecular attosecond electronic dynamics. Extending methods such as attosecond high harmonic generation spectroscopy to complex polyatomic molecules requires an understanding of multielectronic excitations, already hinted at by theoretical modeling of experiments on atoms, diatomics, and triatomics. Here, we present a direct method which, independent of theory, experimentally probes the participation of multiple electronic continua in the SFI dynamics of polyatomic molecules. We use saturated (n-butane) and unsaturated (1,3-butadiene) linear hydrocarbons to show how subcycle SFI of polyatomics can be directly resolved into its distinct electronic-continuum channels by above-threshold ionization photoelectron spectroscopy. Our approach makes use of photoelectron-photofragment coincidences, suiting broad classes of polyatomic molecules.     

Feline idiopathic inflammatory bowel disease: what we know and what remains to be unraveled

PRACTICAL RELEVANCE: Feline idiopathic inflammatory bowel disease (IBD) denotes one form of chronic enteropathy that is immunologically mediated and characterized by persistent or recurrent gastrointestinal (GI) signs and histologic inflammation. Signs of vomiting, diarrhea and weight loss generally predominate, and mucosal inflammation may occur in any portion of the GI tract (especially the small intestine). Affected cats may also have concurrent inflammation in other organs, such as the pancreas and liver, which may impact clinical disease severity. CLINICAL CHALLENGES: The exact etiologies of this heterogeneous group of disorders have yet to be determined, though results from basic science and clinical studies suggest that interplay between genetic factors and enteric bacteria is crucial for disease development. The diagnosis is one of exclusion and requires intestinal mucosal biopsy to characterize the type and severity of the inflammatory infiltrate, and to differentiate IBD from other disorders, including alimentary lymphoma. Controversy exists concerning the relative diagnostic accuracy of endoscopic versus full-thickness specimens for the diagnosis of IBD and its differentiation from alimentary lymphoma. AUDIENCE: This article is intended to provide veterinary practitioners with a comprehensive clinical update on idiopathic IBD in cats. It reviews the current evidence-based data, the diagnostic approach, the evolving histologic criteria, and treatment options and outcome for feline patients with this syndrome.     

Increased broiler muscle carnosine and anserine following histidine supplementation of commercial broiler feed concentrate

Abstract

A feeding experiment involving histidine supplementation to broiler feed resulted in increased concentration of the histidine containing dipeptides anserine and carnosine in broiler breast muscle. Supplementation with 1 g histidine per kg feed gave a 64% increase in carnosine, and about 10% increase in anserine in the muscle. The standard broiler feed concentrate now in use in Norway seems to contain less histidine than what may be needed for optimal synthesis of carnosine and anserine. These dipeptides have important roles as antioxidants, pH buffering agents and anti-glycation agents. They may have important roles in meat for increasing its stability, shelf life and antioxidant capacity, and it might be speculated that broiler meat rich in anserine and carnosine in the future will be considered a type of functional food, having possible health-beneficial effects. Histidine supplementation of standard Norwegian broiler feed concentrate should be considered.     

近自然森林经营在德国的应用成效分析

[目的]分析德国推行近自然森林经营20年来的经营效果,总结德国推行近自然森林经营的经验。[方法]在明晰近自然森林经营原则的基础上,基于两期(间隔期10年)资源清查数据对比,对德国大面积推行近自然森林经营20年的经营效果进行分析。[结果]两期资源清查数据表明,德国采用近自然经营20年后,针叶林如挪威云杉和欧洲赤松的面积显著减少,阔叶林和针阔混交林面积持续增加;阔叶树种蓄积平均增长量约为15 m~3·hm~(-2),针叶树种蓄积平均增长4 m~3·hm~(-2),挪威云杉是唯一蓄积下降的树种,下降了5 m~3·hm~(-2);近自然度等级变化表明,人工林面积在减少,而近自然森林的面积在增加,甚至符合原始林等级的森林面积也在增加。[结论]实现近自然森林经营的目标是一个长期的过程,联邦政府促进和保护森林的政策以及林场主所采用的先进的森林经营技术也是德国森林每公顷蓄积达到336 m~3的重要原因;在近自然森林经营的原则下,德国森林中针阔混交林比例显著增加;将近自然程度不高的人工林经营转化为近自然的森林生态系统是德国森林经营所面临的主要问题。     

The mobility of the trace metals copper,zinc, lead,cobalt, and nickel in tropical estuarine sediments,Ebrie Lagoon,Côte d’Ivoire

Journal of Soils and Sediments - Trace metals accumulate in the food web and can pose high risks to human health and aquatic biota. We investigated seasonal and spatial variations of Cu, Zn, Pb,...     

Shortening the generation cycle in faba bean (Vicia faba) by application of cytokinin and cold stress to assist speed breeding

The aim of this study was to reduce the length of the breeding cycle for faba bean by accelerating seed setting. We examined the mode and time of exogenous 6-benzylaminopurine (BAP) (cytokinin) application, and cold treatments and their combinations in two faba bean genotypes. Acropetal node number of pod and seed set and pollen viability were recorded during the experiments. Application of BAP improved pollen germination. The application of 10^–5 M BAP 4 days after flowering increased seed set at the lower nodes. Cold treatment (8/4°C day/night for 2 days) after the onset of flowering induced the formation of more pods and faster pod set compared to the non-cold treatment. The time to first seed was significantly reduced, and pollen viability was increased in plants exposed to cold treatment. Increased pollen viability also showed a significant positive correlation with seed setting. The combinations of 10^–5 BAP and cold treatment together had similar and independent effects. These results will accelerate plant breeding in faba bean by providing additional tools for reducing generation time.