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71.
对黏虫具有杀虫活性的Bt蛋白筛选及分析 总被引:1,自引:0,他引:1
为筛选对黏虫[Mythimna separata(Walker)]具有毒杀作用的苏云金芽胞杆菌杀虫蛋白,本研究提取Cry1类、Cry2类、Cry9类及Vip3A类等11种蛋白,通过人工饲料喂毒方法对黏虫进行生物活性测定。结果表明,Cry1Ac、Cry1Ab、Cry2Ab、Cry1Be及Cry1Bb蛋白对黏虫具有杀虫活性,其致死中浓度依次为5.09、17.71、26.75、27.42及43.93μg/g;Cry9Aa、Cry9Eb、Cry9Ee蛋白对黏虫生长具有抑制作用,其浓度为10μg/g时的体重抑制率分别为78.4%、79.0%、86.9%,浓度为100μg/g时的体重抑制率分别为92.8%、95.7%、96.7%;Cry1Ba、Cry1Ca和Vip3Aa蛋白对黏虫无显著活性。本研究为黏虫的生物防治奠定了基础,并为抗虫转基因作物的研究提供优良的候选基因。 相似文献
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为提高机械式自动变速器(AMT)换挡品质并缩短换挡过程动力中断时间,提出了一种电磁直线执行器直接驱动的AMT换挡机构。换挡机构运动总质量为1.37 kg,较传统全电式AMT换挡机构总质量(约1.78 kg)降低23%。对换挡过程进行了分段研究,并在换挡过程数学模型和AMT试验台架基础上,验证了设计方案的可行性。换挡机构换挡过程中无选挡操作,可实现挡位切换时退、进挡同时进行的功能,转速差为500 r/min、转动惯量为0.03 kg·m2,换挡时3挡至4挡的换挡时间约为120 ms,提升了AMT的换挡品质,且仍有进一步提高的余地。研究表明,电磁直线执行器直接驱动的AMT换挡机构可实现自动换挡功能。 相似文献
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从数字图书馆与知识产权的概念、数字图书馆的建设和数字图书馆的合理使用等 几个方面对数字图书馆建设中的知识产权保护问题进行了探讨与分析。
相似文献76.
以SCIE数据库为资料来源,统计1998-2005年蛋白质组学研究 论文的高产国家、机构、发表论文期刊及文献主题等相关指标,揭示蛋白质组学的研究现状 ,并对论文数量和影响力进行比较,以衡量我国在蛋白质组领域的研究实力。
相似文献77.
Taking the extended Mindlin solution of a vertical point load acting in the interior of a multi layered elastic medium as the basic solution, an indirect boundary element method (BEM) was adopted to analyze pile foundations embedded in a multi layered elastic soil. The numerical analysis was carried out by the compiled program for a pile foundation in an homogeneous soil and a pile foundation in a multi layered soil. Reasonably good agreement is obtained between the present and existing solutions. Compared with the FEM software ABAQUS, the computational speed of BEM is much faster than that of FEM. Applying BEM in practice is convenient. It is pointed out that the exact measurement of elastic modulus is the key factor for applying BEM in geotechnical engineering. 相似文献
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To solve the key problems of the electric drive system, a controller area network(CAN) based electric drive system for electric vehicles was designed. The system was composed of two parts: a CAN communication module and a motor control module. The newest transceiver (TJA1040) and a stand alone CAN controller were used during the design of the CAN communication module, and anti jamming measures were employed to guarantee communication stability. The fuzzy vector control method and direct speed control were applied to realize highly robust control functions. This system has high compatibility. It can be installed in electric vehicles as an independent module and be extended through CAN Bus. 相似文献
79.
Methods of IF difference detection combined with rapid acquisition synchronization and tracking principle by parallel processing methods were proposed in order to quickly achieve synchronous digital capture and tracking in π/4 DQPSK demodulation.Field program grid array (FPGA) technology to achieve rapid detection of synchronous capture and symbol tracking was adopted by using the improved algorithm in parallel with signal acquisition and frequency offset acquisition. This adoption took into account the characteristics of π/4 DQPSK and was in accordance with the detection of rapid acquisition synchronization and the tracking principle. The experimental results show that clock synchronization signals can be restored stably in eight symbol bit, accurately realize bit timing, and possess strong anti interference capability without affecting frequency differences. This proposed algorithm is suited for frequency hopping communication systems with high transmission rates. 相似文献
80.
Three typical commercial buildings in Chongqing, P.R. China were selected to study the actual summertime operation and energy situations of heating ventilating and air conditioning (HVAC) systems in commercial buildings. Based on tests of energy consumption of HVAC systems in these three buildings, we analyzed the electricity consumption of the main components of the air conditioning systems. The energy efficiency of the chillers and air conditioning water distribution systems were studied. The indoor thermal environment in these three buildings were also tested and discussed. It is concluded that problems exist in the air conditioning systems of commercial buildings. Among the problems are over sized system design, low equipment operating efficiencies, poor operation management, and lack of indoor temperature controls. To reduce energy consumption of HVAC systems in commercial buildings, we propose energy efficient strategies such as sizing systems based on accurate load calculation and automatic control under partial load conditions. 相似文献