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71.
Supplemented Morus nigra extract‐based medium associated with FSH enables the survival and growth of isolated ovine secondary ovarian follicles 下载免费PDF全文
AYP Cavalcante TLBG Lins JMS Santos VRP Barros APO Monte RS Barberino JRGS Almeida MHT Matos 《Reproduction in domestic animals》2018,53(2):423-432
The effects of Morus nigra ethanolic extract, without or with addition of supplements associated or not with FSH, on in vitro culture of ovine secondary follicles were evaluated. In experiment 1, isolated secondary follicles were cultured for 12 days in α‐MEM alone (control) or in different concentrations of M. nigra extract (MN 0.025; 0.05 or 0.1 mg/ml). In experiment 2, culture media were α‐MEM supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine and ascorbic acid (α‐MEM+) or this medium associated with FSH (α‐MEM+ + FSH), or 0.1 mg/ml M. nigra without supplements (MN 0.1) or supplemented (MN 0.1+) without or with FSH (MN 0.1+ + FSH). In experiment 1, 0.1 mg/ml M. nigra showed the highest percentages (p < .05) of normal follicles and fully grown oocytes, besides a higher follicular diameter than α‐MEM and other M. nigra extract concentrations. Moreover, MN 0.1 showed lower (p < .05) glutathione (GSH) levels and similar (p > .05) mitochondrial activity compared to α‐MEM. In experiment 2, MN 0.1+ + FSH showed similar results (p > .05) to α‐MEM+ + FSH for all parameters evaluated, except for the daily growth rate, which was higher (p < .05) in MN 0.1+ + FSH. The GSH levels were higher in MEM+ than all treatments. In addition, oocytes from follicles cultured in MN 0.1+ + FSH showed ability to resume meiosis. In conclusion, M. nigra extract (0.1 mg/ml) added by supplements and FSH can be an efficient medium for ovine secondary follicle development. 相似文献
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Simulation of the economic impact of transmissible gastroenteritis on commercial pig production in Australia 总被引:1,自引:0,他引:1
A simulation model (AUSPIG) was used to predict the effect of an increase in piglet deaths, and a reduction in growth rate and an increase in feed conversion ratio of grower pigs on the profitability of two herds representative of the Australian pig industry caused by the introduction of transmissible gastroenteritis virus (TGE) into those herds. For each herd, mortality rates for piglets under 1 week of age of 50% and 95% were assumed to represent a 'moderate' and a 'severe' outbreak, respectively. A reduction in net revenue of 70% was predicted to occur in the 6 months after a 'moderate' outbreak of TGE (100% for a 'severe' outbreak). This represents a total loss of between $260 and $330 per breeding sow in the 12 months after infection with the TGE virus. The likely financial impact of an outbreak of TGE on an Australian piggery is substantial and should be considered when addressing quarantine issues. 相似文献
74.
The efficacy of diazinon impregnated ear tags against buffalo fly and resulting weight gains and diazinon residues in meat and milk 总被引:1,自引:0,他引:1
Ear tags impregnated with 20% diazinon were evaluated for their efficacy against the buffalo fly (Haematobia irritans exigua) on beef cattle in southern Queensland. Buffalo fly numbers and weight changes were recorded and diazinon residues in tissues of beef cattle and milk from lactating dairy cattle were assayed at different time intervals after tagging. In 2 efficacy trials conducted over 19 and 20 weeks, the mean numbers of buffalo fly on cattle each fitted with ear tags were 1 to 9 and 0 to 16, respectively, in trials 1 and 2, compared with 44 to 345 and 26 to 306 per head on untreated herds, respectively, despite regular spraying of the untreated herd in trial 1 with cyper-methrin to reduce fly burdens. Percentage buffalo fly control was 96.7 to 99.5% and 89.3 to 100% in the 2 trials. Cattle fitted with ear tags gained an average of 94 kg body weight after 5 months compared with 61 kg in the untreated herd, a net increase of 60% in treated animals compared with 28% in the untreated herd. Mean diazinon residue concentrations in the fat of perianal tissue biopsies were 0.02 to 0.03 mg/kg 1 to 8 weeks after tagging. Mean diazinon residue concentrations in the butterfat of milk from lactating dairy cattle were 0.01 to 0.04 mg/kg after tagging. 相似文献
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Objective To compare serum and skin surface IgA concentrations from atopic and normal dogs.
Procedure IgA concentrations in sera and skin washings of 20 clinically normal dogs that had no history of pruritus or skin disease were compared to those obtained in 20 dogs with a diagnosis of atopy determined by history, clinical examination and positive intradermal skin test.
Results There was no significant difference in the mean serum IgA concentration in normal dogs (252 ± 187 mg/L) versus atopic animals (314 ± 327). When skin washings from all sites in both groups were compared, atopic dogs had significantly greater concentrations of IgA in their skin washings than normal dogs as evaluated by an enzyme-linked immunoassay (P < 0.001). However, there was no significant difference between the individual sites of the skin washings of atopic and normal dogs.
Conclusion IgA concentrations of skin washings in atopic dogs were greater than in normal dogs. Further investigations need to determine if the greater concentrations were caused by nonspecific inflammation or by secretion of allergen-specific IgA onto the skin surface. 相似文献
Procedure IgA concentrations in sera and skin washings of 20 clinically normal dogs that had no history of pruritus or skin disease were compared to those obtained in 20 dogs with a diagnosis of atopy determined by history, clinical examination and positive intradermal skin test.
Results There was no significant difference in the mean serum IgA concentration in normal dogs (252 ± 187 mg/L) versus atopic animals (314 ± 327). When skin washings from all sites in both groups were compared, atopic dogs had significantly greater concentrations of IgA in their skin washings than normal dogs as evaluated by an enzyme-linked immunoassay (P < 0.001). However, there was no significant difference between the individual sites of the skin washings of atopic and normal dogs.
Conclusion IgA concentrations of skin washings in atopic dogs were greater than in normal dogs. Further investigations need to determine if the greater concentrations were caused by nonspecific inflammation or by secretion of allergen-specific IgA onto the skin surface. 相似文献
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基于RS和GIS的柘皋河流域景观空间格局研究 总被引:2,自引:2,他引:0
在RS和GIS技术的支持下,运用景观生态学原理,以2000年LandsatTM 7遥感数据为数据源,通过影像解译和实地调查获得柘皋河流域景观类型图,并采用景观指数分析法对流域内景观空间格局做定量分析,为流域内生态保护和区域经济的可持续发展提供有益参考。结果显示:柘皋河流域是以农业为主的发展模式。景观异质性高,分布相对均匀;耕地破碎化程度低,连通性好,利于农业发展;居民点与交通用地破碎化程度高,边缘效应强,对周围影响大。A区农业化和非农化矛盾最强烈。B区景观异质性高,景观类型分布均匀,生态结构稳定。C区景观异质性低,生态稳定性较差。 相似文献
79.
S Chen RS Ingram MJ Hostetler JJ Pietron RW Murray TG Schaaff JT Khoury MM Alvarez RL Whetten 《Science (New York, N.Y.)》1998,280(5372):2098-2101
A transition from metal-like double-layer capacitive charging to redox-like charging was observed in electrochemical ensemble Coulomb staircase experiments on solutions of gold nanoparticles of varied core size. The monodisperse gold nanoparticles are stabilized by short-chain alkanethiolate monolayers and have 8 to 38 kilodaltons core mass (1.1 to 1.9 nanometers in diameter). Larger cores display Coulomb staircase responses consistent with double-layer charging of metal-electrolyte interfaces, whereas smaller core nanoparticles exhibit redox chemical character, including a large central gap. The change in behavior is consistent with new near-infrared spectroscopic data showing an emerging gap between the highest occupied and lowest unoccupied orbitals of 0.4 to 0.9 electron volt. 相似文献