辣木叶蛋白质提取工艺研究

以辣木叶粉为原料,采取复水、均质、浸提及等电点法,提取其中的蛋白质。经研究得出辣木叶蛋白质提取的工艺条件为:水粉比100∶1,提取温度50~60℃,提取时间50~60 min,等电点分离pH值为4.0~4.5。依此可使产品的蛋白质含量达68.86%,蛋白质提取率达70%。     

思茅松等位酶实验方法及改进

以思茅松种子的胚乳为实验材料,探索了思茅松等位酶的实验方法.采用聚丙烯酰胺垂直板凝胶电泳与水平切片淀粉凝胶电泳相结合的方法,获得所试验的13种等位酶中AAT,ADH,EST,GDH,G6PD,MDH,PGD6,PGM和SKD等9种电泳效果良好的等位酶酶谱.通过两种电泳方法的有机结合,以及对制样及上样方法的改进,大大地提高了实验效率.本研究得到的等位酶酶谱式样,为下一步思茅松种群遗传分析提供了资料.     

New steroids from Clerodendrum colebrookianum

Five new steroids, colebrin A-E (1-5) were isolated from the aerial parts of Clerodendrum colebrookianum. The structures of the new compounds were elucidated on the basis of spectral evidence.     

虫生真菌粉拟青霉生产工艺

对虫生真菌粉拟青霉的生产工艺流程,生产步骤、方法,各个生产环节中的质量检测以及产品的包装、贮存、运输进行了详尽论述,为该菌的规模化生产及其防治应用作参考。     

外来种互花米草对江苏省潮间带生物地球化学过程的影响研究

Spartina alterniflora Loisel, a species vegetating in intertidal flats along the eastern coast of the United States, was introduced in China almost 30 years ago and has become an urgent topic due to its invasiveness in the coastal zone of China. The impacts of this alien species S. alterniflora on intertidal ecosystem processes in the Jiangsu coastland were investigated by comparing the sediment nutrient availability and trace element concentration characteristics in a mud flat and those of a four-year old Spartina salt marsh that had earlier been a mudflat. At each study site, fifteen plots were sampled in different seasons to determine the sediment characteristics along the tidal flats. The results suggested that Spartina salt marsh sediments had significantly higher total N, available P, and water content, but lower pH and bulk density than mudflat sediments. Sediment salinity, water content, total N, organic C, and available P decreased along a seaward gradient in the Spartina salt marsh and increased with vegetation biomass. Furthermore, the concentrations of trace elements and some metal elements in the sediment were higher under Spartina although these increases were not significant. Also, in the Spartina marsh, some heavy metals were concentrated in the surface layer of the sediment. The Spartina salt marsh in this study was only four years old; therefore, it is suggested that further study of this allen species on a longer time frame in the Jiangsu coastland should be carried out.     

真实收益学说的形成、发展与影响

公允价值和资产负债观已成为现阶段各国会计准则和国际会计准则的显著特征,本次金融危机中各方的指责也未能动摇这一局面,这就表明公允价值与资产负债观必然具有理论上的合理性。而要明确公允价值与资产负债观到底具有怎样的理论合理性,并理清公允价值、资产负债观、全面收益、资本保全观念以及会计准则管制之间存在的关系,就必须对真实收益学说的发展背景、发展阶段和主要观点进行考察。通过这一考察,我们不难发现公允价值、资产负债观与全面收益实际上是一个同一体,且这个同一体与资本保全观念统一于真实收益学说,而会计准则管制的产生则在相当大程度上是受到了真实收益学说的支持。     

剑川县野生菌资源及可持续发展潜力研究

剑川县有丰富的野生菌资源。调查发现该县常见的野生菌有166种,79属,65.7%是菌根菌,其中美味牛肝菌(Boletu edulis)、铜色牛肝菌(B.aereus)、茶色牛肝菌(B.brunneissimus)、灰褐牛肝菌(B.griseus)、松苞菇(Catathe-lasma ventricosum)、松茸(Tricholoma matsutake)、变绿红菇(Russula virescens)、松乳菇(Lactarius deliciosus)、红汁乳菇(L.hatsudake)、鸡油菌(Cantharellus cibarius)、黄鸡油菌(C.aurora)、羊肚菌(Morchella sp.)是主要的贸易真菌。同时在调查中注意到剑川野生菌资源存在过度利用、不科学采集及违规贸易等问题,针对出现的问题提出了改进的方法及可持续发展建议。     

Seed dormancy and germination of Michelia yunnanensis (Magnoliaceae)

Michelia yunnanensis Franch. is a Chinese endemic ornamental shrub with potential for greater utilization as a landscape and medicinal plant if propagation was less difficult. Seed development and breaking of seed dormancy were investigated to improve propagation of M. yunnanensis. No fresh seeds germinated when tested at the time of dispersal. Newly matured seeds of M. yunnanensis contained differentiated linear underdeveloped embryos that were physiologically dormant. The embryo/seed length ratio of M. yunnanensis was 0.15. Warm stratification did not break seed dormancy. Dormancy was broken by cold stratification at 4 °C but not by flowing water or nitrate. Embryos developed grew inside seeds during cold stratification at 4 °C. In newly harvested dormant seeds, embryos were 0.94 mm long and increased in length 139% before radicle emergence (germination). GA₃ substituted for cold stratification to break dormancy in seeds of M. yunnanensis incubated at 25 °C or 20/25 °C. Mature M. yunnanensis seeds exhibited intermediate complex morphophysiological dormancy. Optimal germination of non-dormant seed in terms of both germination percentage and rate occurred at 20/25 °C.     

矿质营养对主要观赏兰科植物开花影响的研究进展

兰花是极具观赏价值的一类花卉。在人工栽培过程中,可通过调节矿质营养的种类和配比来改善开花质量,调节开花时间,进一步提高兰花的商品价值。现从矿质元素的种类、配比、用量以及施用时间等方面综述矿质元素对主要观赏兰科植物开花的影响,为兰花的栽培生产实践提供依据。     

3个优良薄壳山核桃品种在云南中南亚热带的引种表现

对云南中、南亚热带区域引种栽培的3个薄壳山核桃优良品种进行系统观测和分析.结果表明,3个优良品种在云南中、南亚热带区域表现良好,丰产性、生态适应性和抗病虫危害性较强,坚果品质优良,营养成分丰富.提出薄壳山核桃在云南省该区域内的适生环境,为该区域大力发展种植抛尼(Pawnee)、卡多(Caddo)、肖尼(Shawnee)3个薄壳山核桃优良品种提供重要依据.