双柏县农业现状与发展对策

发展好农业是建设小康社会的物质基础,双柏县农业还处于低层次发展阶段,如果这种局面不能根本改变,很难有力支撑全县经济社会又好又快发展,笔者从自己从事的工作出发,对加快农业可持续发展提出了建议。     

龙眼TIR1和ABP1基因的克隆及其在体胚发生过程中的表达分析

AIM: To investigate the expression and the role of histone deacetylase 8 (HDAC8) in cardiac hypertrophy and the effects of valproic acid sodium (VPA, a histone deacetylase inhibitor) on the expression of HDAC8 and cardiac hypertrophy in two-kidney two-clip (2K2C) renovascular hypertensive rats. METHODS: Male SD rats were randomly divided into sham operation group, 2K2C group, high dose VPA (400 mg穔g^-1穌^-1) treatment group, low dose VPA (200 mg穔g^-1穌^-1) treatment group and candesartan (10 mg穔g^-1穌^-1) treatment group.Four weeks after surgery,the rats were intraperitoneally injected with VPA for 4 weeks. Sham operation and 2K2C rats were given vehicle for 4 weeks. All animals were sacrificed 8 weeks after surgery. The ratio of left ventricular weight to body weight (LVW/BW) was calculated and pathological changes of the myocardium were observed with HE staining. The mRNA expression of atrial natriuretic factor (ANF) and HDAC8 was examined by RT-PCR. The protein level of HDAC8 was also measured by Western blotting analysis. RESULTS: Compared with the control rats, the mRNA and protein expression of HDAC8 significantly increased in the myocardium in 2K2C rats while the mRNA and protein expression of HDAC8 was significantly decreased by VPA treatment in 2K2C rats. The mass index (as measured by LVW/BW) and cardiomyocyte cross areas were markedly increased and myocardial fibers were disordered in 2K2C rats, but these parameters were markedly reversed after treated with VPA for 4 weeks, indicating that VPA attenuated cardiac hypertrophy. Moreover, VPA also decreased the mRNA expression of ANF. CONCLUSION: HDAC8 may play an important role in cardiac hypertrophy in 2K2C renovascular hypertensive rats. VPA inhibits the expression of HDAC8 and prevents the development of cardiac hypertrophy.     

龙眼胚性愈伤组织ACC合成酶基因（DL-ACS1）的克隆及表达分析

以龙眼(Dimocarpus longan Lour.)胚性愈伤组织为材料,采用同源克隆和RACE方法分离得到龙眼胚性愈伤组织乙烯合成限速酶DL-ACS1(1-aminocyclopropane-1-carboxylate synthase)基因的cDNA全长序列(GenBank,FJ617537),共1 817 bp,包含了5’非编码区(73 bp),开放阅读框(1 437 bp),3’非编码区(307 bp)。该cDNA开放阅读框的氨基酸序列(含478个氨基酸)与其它植物ACS具有77%～63%同源性,属于ACC Synthase家族。实时荧光定量PCR分析表明,该基因在龙眼体细胞胚胎(以下简称龙眼体胚)各阶段的表达量存在较大的时期差异,其中,球形胚(Stage 5)的表达量最高,鱼雷形胚(Stage 7)的表达量最低。     

不同栽培条件对蝴蝶兰童期生长的影响

研究不同的肥料、光照、温度和湿度栽培条件对蝴蝶兰童期生长的影响.结果表明:(1)在蝴蝶兰的童期生长过程中,应以喷施含N高的"花宝5号(N∶P∶K=30∶10∶10)"600倍液、"花多多(N∶P∶K∶20∶20∶20)"2000倍液为宜;(2)室外双层遮荫网早晚打开,每天遮荫10h,可有效调整蝴蝶兰生长需要的透光度;(3)当温度控制在20～28℃时,蝴蝶兰苗几乎全年都处于生长状态,生长迅速,长势良好;(4)空气相对湿度为70%～80%,最适宜蝴蝶兰的生长发育.     

虎尾兰吸收甲醛及其保护酶活性的初步研究

通过对不同甲醛浓度处理的密闭容器中甲醛含量、虎尾兰植株体内甲醛含量及其保护酶活性的测定,探讨虎尾兰吸收空气中甲醛的效果,并分析不同甲醛浓度处理后植株体内甲醛含量与保护酶活性的差异性.结果表明:虎尾兰吸收甲醛量随着甲醛处理浓度的提高而增加,当甲醛处理浓度为0.347 mg·m-3、0.431 mg·m-3、0.543 mg·m-3、0.672 mg·m-3、0.884 mg·m-3,虎尾兰吸收甲醛量分别占容器中甲醛总量的14.98%、20.64%、30.76%、39.43%、45.14%;不同处理虎尾兰植株体内甲醛含量差异不显著,且其含量随着处理时间呈波动性变化态势;各处理的虎尾兰植株体内保护酶(POD、SOD和CAT)活性差异不显著.由此推断,虎尾兰吸收的甲醛没有在植株体内积累,甲醛处理对虎尾兰植株影响不显著.     

枇杷细胞悬浮培养生产熊果酸的调控

以枇杷悬浮培养细胞的生长量及熊果酸（UA）的含量为考察指标,从蔗糖浓度、装液量、pH值、接种量、温度、光照强度、摇床转速、激素等开展培养参数的筛选。结果表明：接种量8 g/100 mL,pH6.0,蔗糖30 g/L,25 ℃,白炽灯400 lx,转速130 r/min,装液量130 mL/250 mL,MS+NAA 0.5 mg/L,15 d收获,细胞鲜重是起始的3.577倍,UA含量为34.993 mg/g DW,是自然界枇杷叶的约3.5倍。     

‘紫岩素’大花蕙兰与墨兰3个品种杂交坐果的研究

为筛选出易栽培种植开花、耐高温且抗性强的大花蕙兰新种质,开展了‘紫岩素’大花蕙兰与墨兰品种‘金咀’、‘企剑白墨’和‘企剑黑墨’的杂交研究。结果表明：正交和反交的坐果率差异较大;以‘紫岩素’为母本、墨兰作父本杂交的坐果率高,授粉后第210天,与 ‘金咀’、‘企剑白墨’和‘企剑黑墨’的杂交坐果率分别为81.5％、89.8％和78.0％;以墨兰为母本、‘紫岩素’作父本杂交时落果严重,授粉后第210天,‘金咀’、‘企剑白墨’和‘企剑黑墨’的坐果率分别只有0.0％、0.0％和10.3％;‘紫岩素’与墨兰3个品种正交和反交的果荚,其果长及果宽的生长发育曲线均呈“S”型;以‘紫岩素’为母本的各杂交组合果长于授粉后100～210 d达到最大值（≈8.70 cm）,果宽于授粉后180～210 d达到最大值（≈18.65 mm）;以‘紫岩素’为父本的各杂交组合果长于授粉后120～210 d时达到最大值(≈4.20 cm),果宽于授粉后150～210 d时达到最大值（≈8.60 mm）。     

留叶配合IBA处理对三角梅插穗生根及生理生化指标的影响（摘要）

[目的]探讨留叶配合IBA浸穗处理对三角梅插穗生根及生理生化指标的影响。[方法]在种苗智能化快繁系统调控下,设置三角梅插穗留叶与去叶类型各配合IBA、清水浸穗的4种处理,研究留叶配合IBA处理对三角梅插穗生根及生理生化指标的影响。[结果]留叶类型插穗的可溶性糖含量较高,不定根分化前期吲哚乙酸氧化酶活性较低,插穗生根数、根长度、根生长量及成活率均明显高于去叶类型;与清水浸穗相比,500×10^-6IBA浸穗提高了插穗的可溶性糖含量,促进了插穗生根。[结论]在三角梅种苗智能化快繁中,留叶插穗配合插前500×10^-6IBA浸穗处理可明显促进插穗生根,提高成活率。     

植物开花的激素调节（一）

高等植物花的发端,标志着其营养生长向生殖发育的重要转变,这是植物生命周期中最重要的活动之一.许多研究者对植物开花生理学作了大量的研究.正如Lang(1961、1965)、Searle(1965)、Evans(1971)和Zeevart(1976)的评述论文,以及由Evans(1969b)、Beraier(1970)和CNRS(1979)主编的专著,都对此作出了有价值的贡献