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61.
Natural sawdust of Calabrian pine was explored as low-cost industrial by-product for a hetero-bireactive dye removal from aqueous solution in this study. Batch experiments were carried out to investigate the effects of contact time and dye concentration on biosorption efficiency. Maximum biosorption amounts were achieved almost in the 20-30 min of contact for the studied dye range (50-200 mg l -1). An increase in the dye concentration resulted in decrease in the percent dye removal and increase in the biosorption capacity of biosorbent. Different kinetic and isotherm models were used to examine the biosorption experimental data for elucidating the dye removal mechanism. The equilibrium data were best represented by Freundlich isotherm model confirming multilayer coverage. The kinetics of dye biosorption best obeyed the pseudo-secondorder equation. The negative value of standard Gibbs free energy change (-3.61 kJ mol-1) indicated that physical forces were involved in the spontaneous dye biosorption. Thus, the sawdust as a possible non-conventional biosorbent presented an interesting option for bioremediation of contaminated environments by such unsafe dyes. 相似文献
62.
Rita Maria Alves de Moraes Tais Cristina Bastos Soares Lucinete Regina Colombo Maria Fernanda Spegiorin Salla Josie Gomes de Almeida Barros Newton Deniz Piovesan Everaldo Gonçalves de Barros Maurilio Alves Moreira 《Euphytica》2006,149(1-2):221-226
Summary The antinutritional factors found in soybean, lectin (SBL) and Kunitz trypsin inhibitor (SKTI), are usually inactivated by heat treatment. However, residual activity of these factors can be found in several types of soy-derived products. Heat treatment does not completely eliminate these factors, and in addition it may considerably decrease protein solubility. The genetic elimination of these antinutritional factors could be an alternative to the heat treatment. This study aimed to develop soybean lines devoid of SKTI and SBL in their seeds. The population under study was obtained by crossing the normal cv. Monarca with a soybean line lacking SKTI and SBL. Specific DNA primers were designed for the identification of the recessive alleles that condition the absence of SKTI and SBL. F2 seeds presenting the DNA markers that identify the recessive alleles were selected and the corresponding F2 plants were backcrossed with the recurrent parent (‘Monarca’), producing the BC1F1 generation. The F2 generation was analyzed by SDS-PAGE in order to confirm the genotypes of the F2 selected. The segregation tests confirmed that these traits are governed by two genes that segregate independently. 相似文献
63.
Lucimara Chiari Lucas Koshy Naoe Newton Deniz Piovesan Inês Chamel José Cosme Damião Cruz Maurilio Alves Moreira Everaldo Gonçalves de Barros 《Euphytica》2006,150(1-2):141-147
Summary We studied the genetic basis of isoflavone content inheritance in soybean seeds. The progenitors BARC-8 (low isoflavone content), IAC-100 (high isoflavone content), the F1 and F2 populations derived from reciprocal crosses, and backcross populations were analyzed for isoflavone content and composition. Six isoflavones were detected: daidzin (DZ), genistin (GT), glycitin (GC), malonyldaidzin (MDZ), malonylgenistin (MGT) and malonylglycitin (MGC). DZ, GT, MDZ and MGT contents were influenced by the cytoplasm and the nuclear genes of the maternal parent. For this reason, a genetic model was considered that included the cytoplasmic effect and epistasis between nuclear and cytoplasmic genes. Except for GT, the additive effect was the most important one. For GT content the cytoplasmic effect was the most important. Except for MDZ, the epistatic effects were significant for all the isoflavone forms. Our data indicate that genetic improvement for these traits should explore the additive genetic variances in superior lines or the cytoplasmic effect and the epistatic interactions between cytoplasmic and nuclear genes to obtain the largest selection gains. 相似文献
64.
Lucimara Chiari Newton Deniz Piovesan Lucas Koshy Naoe Inês Chamel José José Marcelo Soriano Viana Maurilio Alves Moreira Everaldo Gonçalves de Barros 《Euphytica》2004,138(1):55-60
Isoflavones are a class of compounds present in high amounts in soybean seeds, which can be used for prevention and treatment
of several chronic diseases. Proteins present in soybean seeds are the basis for the high nutritional value and versatility
of this leguminous species in animal and human feeding. The main goals of this work were to estimate heritabilities for isoflavone
contents in soybean seeds and the correlation between isoflavone and protein contents. Commercial variety IAC-100 (high isoflavone
and normal protein contents) and the line BARC-8 (low isoflavone and high protein contents) were crossed, and one single F1 plant derived 97 F2 seeds, which were used to obtain F3 seeds. A sample of F3 seeds from each F2 plant was used for isoflavone determination by HPLC and protein by the Kjeldahl method. Six isoflavone forms were detected:
daidzin, genistin, glycitin, malonyldaidzin, malonylgenistin and malonylglycitin. Total isoflavone contents ranged from 427.92
to 965.89 μg per gram of dry seed and the protein content ranged from 45.17 to 34.95% in BARC-8 and IAC-100, respectively.
Our results indicate that it is possible to select for high isoflavone content in early breeding generations because the broad
sense heritabilities for the contents of the various isoflavone forms were higher than 90%. In addition, high correlation
values among the contents of the individual isoflavone forms were observed (between 0.80 and 0.98). However, negative correlation
values were obtained between isoflavone and protein contents, ranging from −0.51 to −0.37 for the different isoflavone forms.
The correlation value of −0.47 between total isoflavone and protein contents confirmed the negative correlation between these
two parameters, as reported by other authors.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
65.
66.
Larissa Buedenbender Abhishek Kumar Martina Blümel Frank Kempken Deniz Tasdemir 《Marine drugs》2021,19(1)
Red yeasts of the genus Rhodotorula are of great interest to the biotechnological industry due to their ability to produce valuable natural products, such as lipids and carotenoids with potential applications as surfactants, food additives, and pharmaceuticals. Herein, we explored the biosynthetic potential of R. mucilaginosa 50-3-19/20B collected from the Mid-Atlantic Ridge using modern genomics and untargeted metabolomics tools. R. mucilaginosa 50-3-19/20B exhibited anticancer activity when grown on PDA medium, while antimicrobial activity was observed when cultured on WSP-30 medium. Applying the bioactive molecular networking approach, the anticancer activity was linked to glycolipids, namely polyol esters of fatty acid (PEFA) derivatives. We purified four PEFAs (1–4) and the known methyl-2-hydroxy-3-(1H-indol-2-yl)propanoate (5). Their structures were deduced from NMR and HR-MS/MS spectra, but 1–5 showed no anticancer activity in their pure form. Illumina-based genome sequencing, de novo assembly and standard biosynthetic gene cluster (BGC) analyses were used to illustrate key components of the PEFA biosynthetic pathway. The fatty acid producing BGC3 was identified to be capable of producing precursors of PEFAs. Some Rhodotorula strains are able to convert inulin into high-yielding PEFA and cell lipid using a native exo-inulinase enzyme. The genomic locus for an exo-inulinase enzyme (g1629.t1), which plays an instrumental role in the PEFA production via the mannitol biosynthesis pathway, was identified. This is the first untargeted metabolomics study on R. mucilaginosa providing new genomic insights into PEFA biosynthesis. 相似文献
67.
68.
Giuseppina Chianese Fernando Scala Barbara Calcinai Carlo Cerrano Henny A. Dien Marcel Kaiser Deniz Tasdemir Orazio Taglialatela-Scafati 《Marine drugs》2013,11(9):3297-3308
Chemical analysis of the Indonesian sponge Plakortis cfr. lita afforded two new analogues of the potent trypanocidal agent manadoperoxide B (1), namely 12-isomanadoperoxide B (2) and manadoperoxidic acid B (3). These compounds were isolated along with a new short chain dicarboxylate monoester (4), bearing some interesting relationships with the polyketide endoperoxides found in this sponge. Some semi-synthetic analogues of manadoperoxide B (6–8) were prepared and evaluated for antitrypanosomal activity and cytotoxicity. These studies revealed crucial structure–activity relationships that should be taken into account in the design of optimized and simplified endoperoxyketal trypanocidal agents. 相似文献
69.
Bayçin D Altiok E Ulkü S Bayraktar O 《Journal of agricultural and food chemistry》2007,55(4):1227-1236
The adsorption isotherms of oleuropein and rutin were evaluated at different temperatures, pH values, and solid/liquid ratios. The experimental data of adsorption isotherms were well fitted to a Langmuir model. The maximum adsorption capacities were determined as 108 mg of oleuropein/g of silk fibroin and 21 mg of rutin/g of silk fibroin. After adsorption of oleuropein and rutin, the antioxidant capacity of silk fibroin increased from 1.93 to 3.61 mmol of TEAC/g. Silk fibroin also gained antimicrobial activity against Staphylococcus aureus and Klebsiella pneumoniae after adsorption of olive leaf antioxidants. In a desorption process, 81% of rutin and 85% of oleuropein were removed from the adsorbent surface in 70% aqueous ethanol solution. Consequently, silk fibroin was found to be a promising biomaterial for the production of functional food or dietary supplements and for the purification of oleuropein and rutin from olive leaf extracts. 相似文献
70.