喀斯特地区三叶草混播草地群落组分20年动态

三叶草（Trifolium）混播草地群落持久性研究对喀斯特草地建设与石漠化治理具有重要意义。在威宁灼圃示范牧场开展的由白三叶（T. repens）、红三叶（T. pratense）、无芒雀麦（Bromus inermis）、紫羊茅（Festuca rubra）,分别加上多年生黑麦草（Lolium perenne）、鸭茅（Dactylis glomerata）、黑穗画眉草（Eragrostis nigra）、鹅冠草（Elymus kamoji）组成4个5组分混播草地,在绵羊适度放牧下测定牧前、牧后草地现存量,连续进行20年持久性研究。结果表明：时间及组合×利用时间的交互作用对所有混播组合影响极显著,而组合间产量差异不显著,其动态变化与年降水量的动态变化之间无显著的对应关系,但同物种在各混播群落中的生产力和稳定性差异明显,说明各组分之间存在显著的生态位互补和竞争共存关系。各物种中生产力与稳定持久性最好的是紫羊茅和白三叶,其次是多年生黑麦草和鸭茅,最差的是无芒雀麦和鹅冠草;而在草地建植初期表现最好的是多年生黑麦草和红三叶。     

转基因水稻恢复系及其F1代Bt蛋白的时空表达分析

本研究以抗虫水稻恢复系9311(Bt)及其杂交种F1(Bt)为研究材料,以非转基因的9311为阴性对照,利用ELISA方法研究9311(Bt)及F1(Bt)各生育时期可溶性总蛋白和Bt蛋白的时空变化规律,为转Bt基因抗虫水稻的安全监管提供科学依据。结果表明：外源基因的导入没有引起水稻组织中可溶性总蛋白含量的明显变化;9311(Bt)的Bt蛋白表达量在整个生长周期的各个部位均高于相应的F1(Bt)植株;同一植株不同组织器官中Bt蛋白表达量为：叶片跃胚乳跃颖壳及茎秆跃根;同一植株不同发育期叶片Bt蛋白的测定结果整体表现为：营养生长阶段跃生殖生长阶段跃成熟衰老阶段。研究结果为转Bt基因抗虫水稻适宜检测时期的选择提供了一定的参考。     

太行山山前平原土壤有效水动态分析

利用栾城试验站1998—2002年的降水和土壤水观测资料,探讨太行山山前平原的土壤有效水量和土壤水有效性的时间变化特征。结果表明,①土壤有效水量年内变化明显,受降水影响显著;年际变化呈减少趋势,秋季变化幅度最大;作物的主要供水层下移。②作物根系层土壤水的总体有效性在全年基本为“中效”;在冬小麦生育期,深层的土壤水逐渐转为“难效”,经过雨季能基本得到缓解;自1998年至2001年土壤水分有效性等级由浅层至深层依次降低。     

Short- and long-term implications of clearcut and two-age silviculture for conservation of breeding forest birds in the central Appalachians, USA

Two-age (deferment or leave tree) harvesting is used increasingly in even-aged forest management, but long-term responses of breeding avifauna to retention of residual canopy trees have not been investigated. Breeding bird surveys completed in 1994-1996 in two-age and clearcut harvests in the central Appalachian Mountains of West Virginia, USA allowed us to document long-term changes in these stands. In 2005 and 2006, we conducted point counts in mature unharvested forest stands and in 19-26 year-old clearcut and two-age harvests from the original study and in younger clearcut and two-age stands (6-10 years old). We found differences in breeding bird metrics among these five treatments and temporal differences in the original stands. Although early-successional species are typically absent from group selection cuts, they were almost as common in young two-age stands as clearcuts, supporting two-age harvests as an alternative to clearcutting. Although older harvests had lower species richness and diversity, they were beginning to provide habitat for some species of late-successional forest songbirds that were absent or uncommon in young harvests. Overall, late-successional forest-interior species were more flexible in their use of different seral stages; several species used both age classes and harvest types in addition to mature forest, which may reflect the lack of edges in our heavily-forested landscape. Consequently, two-age management provides habitat for a diverse group of species as these stands mature and may be an ecologically sustainable alternative to clearcutting in landscapes where brown-headed cowbirds (Molothrus ater) are uncommon.     

Temporal shifts in diversity and quantity of nirS and nirK in a rice paddy field soil

Denitrification is an important part of the nitrogen cycle in the environment, and diverse bacteria, archaea, and fungi are known to have denitrifying ability. Rice paddy field soils have been known to have strong denitrifying activity, but the microbes responsible for denitrification in rice paddy field soils are not well known. Present study analyzed the diversity and quantity of the nitrite reductase genes (nirS and nirK) in a rice paddy field soil, sampled four times in one rice-growing season. Clone library analyses suggested that the denitrifier community composition varied over sampling time. Although many clones were distantly related to the known NirS or NirK, some clones were related to the NirS from Burkholderiales and Rhodocyclales bacteria, and some were related to the NirK from Rhizobiales bacteria. These denitrifiers may play an important role in denitrification in the rice paddy field soil. The quantitative PCR results showed that nirK was more abundant than nirS in all soil samples, but the nirK/nirS ratio decreased after water logging. These results suggest that both diversity and quantity changed over time in the rice paddy field soil, in response to the soil condition.     

Plasma levels of a low-dose constant-rate-infusion of ketamine and its effect on single and repeated nociceptive stimuli in conscious dogs

This study quantitatively investigated the analgesic action of a low-dose constant-rate-infusion (CRI) of racemic ketamine (as a 0.5 mg kg⁻¹ bolus and at a dose rate of 10 μg kg⁻¹ min⁻¹) in conscious dogs using a nociceptive withdrawal reflex (NWR) and with enantioselective measurement of plasma levels of ketamine and norketamine. Withdrawal reflexes evoked by transcutaneous single and repeated electrical stimulation (10 pulses, 5 Hz) of the digital plantar nerve were recorded from the biceps femoris muscle using surface electromyography.Ketamine did not affect NWR thresholds or the recruitment curves after a single nociceptive stimulation. Temporal summation (as evaluated by repeated stimuli) and the evoked behavioural response scores were however reduced compared to baseline demonstrating the antinociceptive activity of ketamine correlated with the peak plasma concentrations. Thereafter the plasma levels at pseudo-steady-state did not modulate temporal summation. Based on these experimental findings low-dose ketamine CRI cannot be recommended for use as a sole analgesic in the dog.     

麦田害虫与天敌群落动态变化研究

2008年3月15日至5月29日系统调查了冬小麦田害虫与天敌的种群数量动态,并利用相对丰富度、优势度指数、优势集中性指数、多样性指数和均匀度分析了害虫与天敌群落动态变化及时间生态位。结果表明:麦田昆虫群落组成较为丰富,由11目39科59种昆虫组成;优势种为麦长管蚜(Macrosiphum avenae)、麦圆叶爪螨(Penthaleus major)、龟纹瓢虫(Propylea japonica)和麦蚜茧蜂(Ephedrus plagiator)。群落的总个体数和丰富度随调查时间而变化;多样性指数与均匀度指数在小麦生长前期较低,中期和后期较高,两者变化规律基本一致,而优势集中性指数变化趋势与其相反。在害虫种群中以棉铃虫(Helicoverpa armigera)种内竞争最为激烈,条斑叶蝉(Psammotettix striatus)的竞争最缓和;棉铃虫与麦长管蚜种间竞争最强,其次为麦圆叶爪螨与小麦叶蜂(Dolerus tritici)。在天敌种群中以七星瓢虫(Coccinella septempunctata)种内竞争最为激烈,麦蚜茧蜂的种内竞争最为缓和;龟纹瓢虫分别与七星瓢虫和异色瓢虫(Leis axyridis)在时间维度上的竞争较为激烈,而麦蚜茧蜂分别与龟纹瓢虫、七星瓢虫和异色瓢虫的竞争比较缓和。从害虫种群与天敌种群之间的重叠值看,异色瓢虫和七星瓢虫对棉铃虫的控制作用较强,而麦蚜茧蜂和龟纹瓢虫则对麦田主要害虫麦长管蚜、条斑叶蝉和小麦潜叶蝇(Phytomyza nigra)等的跟随和控制作用均较强。     

淄博市冰雹时空分布特征

利用1977～2006年的降雹资料,对淄博地区冰雹的时空分布特征进行了分析。结果表明,淄博市冰雹集中出现在4～10月,6月份降雹次数最多;降雹时间存在明显的日变化,峰值主要出现在14:00～16:00;降雹持续时间一般为1～15 min,冰雹直径一般在40 mm以下。     

近52年我国各强度降雪的时空分布特征

利用1954～2005年我国674个气象台站的逐日降雨量和天气现象观测资料,构建了全国范围的小雪、中雪、大雪、暴雪和特大暴雪序列,分析了中国各强度降雪的时空变化规律。结果表明：①我国年平均降雪日数超过30天的地区有新疆北部、东北东部与北部、青藏高原东部,全国只有高原和高山地区的年平均降雪日数超过60天;②我国降雪主要集中在11月一次年4月,其中小雪和特大暴雪1月份最多,中雪2月份最多,大雪和暴雪3月份最多;③在51个年度里,1955—1967年度我国降雪处于少雪的负位相,1968—1994年基本为多雪的正位相。1995～2005又为少雪的负位相。     

普兰店冰雹气候统计分析及对农业生产的影响

应用1971～2007年普兰店气象站地面观测资料,统计分析了普兰店冰雹的时空分布特征、范围和强度及冰雹对农业生产的危害,并提出了防御对策。