小麦 TaGRF4基因分析与功能鉴定

旨在研究小麦 TaGRF4基因的生物学功能，利用生物信息学技术分析小麦 TaGRF4基因结构及其与其他作物的进化关系，利用实时定量检测 TaGRF4在不同发育时期叶片和不同区段叶片中的表达丰度，同时检测外源GA处理条件下 TaGRF4的表达情况，最后利用BSMV-VIGS体系使 TaGRF4的3个同源基因同时沉默，研究 TaGRF4沉默对叶片生长发育的影响。结果表明， TaGRF4是玉米 ZmGRF10的同源基因，在幼嫩叶片和叶尖部位的表达量最高，而且对外源GA处理响应迅速； TaGRF4沉默后新生叶片明显伸长，说明 TaGRF4与 ZmGRF10有类似的生物功能，能够抑制叶片的伸长生长。     

不同药剂拌种对小麦茎基腐病的初步研究

小麦是我国的主要粮食作物之一,由于常年秸秆还田及连作,造成小麦茎基腐病病菌有加重趋势,本文选用不同的杀菌剂进行小麦拌种,通过在小麦返青期、灌浆期、成熟期的调查,表明用6%立克秀悬浮种衣剂进行拌种防治小麦茎基腐病效果最好。     

施氮量对豫北冬小麦产量及子粒主要矿质元素含量的影响

以兰考矮早8、豫麦49-198和平安8号为材料,设置5个氮素水平（0、120、180、240、360kg/hm ²）,研究不同施氮量对豫北冬小麦子粒产量及其N、P、K、Ca、Mg、Fe、Mn、Cu、Zn、B等矿质元素含量的影响。结果表明：子粒中N与B（r=0.879）、N与Mg（r=0.858）、Mg与Zn（r=0.871）、Mg与B（r=0.877）含量间相关系数较高。施氮显著提高了子粒N、Ca、Fe、Cu、Zn、B含量,K、Mg含量受施氮量影响较小,但P和Mn含量明显下降。兰考矮早8子粒中各种矿质元素（除B外）含量明显高于平安8号,豫麦49-198介于二者之间。施氮在提高小麦子粒产量的同时降低了P/Ca、P/Mg、P/Fe和P/Zn的值,增强了Ca、Mg、Fe、Zn的生物有效性。此外,研究发现施氮量达到180kg/hm ²后,继续增施氮肥小麦产量难以提升。可见,合理的氮肥管理可以提高豫北地区冬小麦产量及子粒中微量元素的含量;过量施氮不仅难以提高子粒产量,还会降低子粒P和Mn的含量。     

Soil sterilization effects on root growth and formation of rhizosheaths in wheat seedlings

Sterilized soils are frequently used in experiments related to soil biology. Soil sterilization is known to alter physicochemical characteristics of soil, plant growth and community structure of the newly developed bacterial population. However, little information exists regarding soil sterilization effects on belowground processes mediated through root–microbe–soil interactions, e.g., development of rhizosheaths which significantly promote the plant growth under stress environments. The present study was conducted to elucidate effects of soil sterilization on wheat root growth and formation of rhizosheaths in relation to chemical changes caused by soil sterilization and the proportion of expolysaccharide (EPS)-producers in bacterial population recolonizing the sterilized soils. Wheat plants were grown for two weeks under greenhouse conditions either in the unsterilized soil or in soils sterilized by autoclaving (121 °C, 1 h) or by gamma (γ)-irradiation (50 kGy). While soil sterilization had no effect on the release of macronutrients, both sterilization procedures significantly increased the electrical conductivity, water-soluble carbon and DTPA-extractable Mn. Seedlings grown in sterilized soils produced higher root biomass and the rhizosheath soil (RS) mass as compared to those grown in the unsterilized soil. Soil sterilization also increased the root length, surface area, volume and number of tips. In bulk soil, RS and on roots, the proportion of EPS-producers in the total bacterial population was higher in sterilized treatments than in the unsterilized. Amending the unsterilized soil with glucose-C increased the root biomass, whereas adding Mn II increased the RS mass. The results showed that soil sterilization by autoclaving or γ-irradiation increases the root growth and RS mass of wheat seedlings. The water-soluble C and DTPA-extractable Mn released upon sterilization, and the increased proportion of EPS-producers in the bacterial population recolonizing the sterilized soils were involved in the observed effects. The results may have implications in studies using autoclaved or γ-irradiated soils to investigate soil–plant–microbe interactions and signify the need to account for intrinsic stimulatory effects of soil sterilization.     

Diazotroph community structure and abundance in wheat–fallow and wheat–pea crop rotations

Biological input of nitrogen (N) from the atmosphere by free-living diazotrophs can help alleviate fertilizer use in agricultural systems. In this study, we investigated the effect of N fertilizer and winter pea (Pisum sativum L.) crop on the community structure and abundance of free-living diazotrophs in a two year study of dryland winter wheat (Triticum aestivum L.) no-till production system in Eastern Oregon, USA. Based on quantification of the nifH gene, diazotroph abundance was strongly influenced by plant species and the crop year in which the soil samples were collected. A greater amount of nifH copies was recovered in 2012 compared to 2011 either as copies per gram soil or normalized to the abundance of bacterial 16S rRNA genes. The quantity of genes was greater under pea than wheat in 2012 although no difference was observed in the preceding year. The nifH gene abundance was positively correlated to ammonium concentration in 2011 and bacterial abundance in 2012. Nitrogen application did not influence diazotroph abundance in the top 0–5 cm; however the abundance was reduced by application at the lower 5–10 cm depth under wheat crop. The diazotroph community structure appeared to be influenced more by N fertilization rather than plant species with the exception of wheat in 2012. Changes in the community structure over the two years were greater for fertilized than unfertilized soil. Collectively, these data suggest that year-to-year variability had a greater influence on diazotroph communities rather than specific parameters of plant species, fertilization, total N, total organic C, or soil pH. Multi-year studies are necessary to define the specific drivers of diazotroph abundance, community structure and function.     

Economics of foliar fungicides for hard red winter wheat in the USA southern Great Plains

Grain yields of winter wheat (Triticum aestivum L.) in the southern Great Plains are often reduced by the presence of foliar diseases. This study was conducted to determine whether the application of foliar fungicides is an economically optimal management strategy. The effects of fungicide treatment on commercially available hard red winter wheat varieties with differing levels of genetic resistance (i.e., resistant, intermediate, and susceptible) to foliar diseases were investigated at two locations, Apache and Lahoma, OK, USA, for the harvest years 2005–2012. Two fungicides were rotated between the two locations and applied at approximately Feekes growth stage 9–10.5. When averaged across years, plots to which fungicide was applied generated greater average net returns than plots that did not receive fungicide for susceptible varieties at Apache, and for resistant, intermediate, and susceptible varieties at Lahoma. However, foliar fungicide application was not economical in every year at either location suggesting fungicide use should be reassessed each year given that profitability depends on year specific yield potential, prices, and foliar disease conditions. At both locations high disease incidence occurred in all but one site-year when the average March through May relative humidity exceeded 65%. Additional research would be required to determine the relationship between weather, including relative humidity, and disease incidence, and to develop an economic threshold for treatment decision aid.     

Effects of five additive materials on mechanical and dimensional properties of wood cement-bonded boards

There is a growing desire to improve the properties and use of nonwood plant materials as supplements to wood materials for wood cement-bonded boards (WCBs). This study was conducted to determine the comparative properties of WCBs containing various amounts of discontinuous inorganic fiber materials, such as alkali-resistant glass fiber, normal glass fiber, mineral wool, and nonwood plant materials such as retted flax straw and wheat straw particles. Tested cement-bonded boards were made at wood/additive compositions of 100/0, 90/10, 80/20, 70/30, 60/40, and 50/50 (weight percentages). Seventy-eight laboratory-scale WCBs were produced. Various board properties, such as the modulus of rupture (MOR), internal bonding strength (IB), water absorption (WA), thickness swelling (TS), and linear expansion (LE), were studied. The test results showed that three types of discontinuous inorganic fiber used as reinforcing materials in composites significantly enhanced and modified the performance of WCBs. The mechanical properties and dimensional stability of cement-bonded board were significantly improved with increasing amounts of the additives. MOR and IB were increased; and WA, TS, and LE of boards were reduced by combination with the inorganic fiber materials. The results also indicated that combination with retted flax straw particles only slightly increased the MOR of boards, and wheat straw particles led to marked decreases in all the mechanical properties and the dimensional stability of WCBs.Part of this report was presented at the 50th Annual Meeting of the Japan Wood Research Society, Kyoto, April 2000     

麦秆表面硅含量分布的研究

利用ESCA研究了麦秆表面在不同温度下和经氩离子刻蚀后Si元素含量的变化。结果表明：在麦秆横切面上，Si元素含量中间层（接近麦秆外表面）含量较高；随着温度的升高，麦秆表面Si元素含量先降低而后增加，在选定的几个温度中，170℃和220℃时Si含量最低；麦秆表面经氩离子刻蚀后，由外及里，随着氩刻的加深，Si含量呈现升高趋势。麦秆中Si元素存在的化学环境与聚甲基硅氧烷中Si的化学环境比较接近。     

麦秸/聚氨酯复合人造板的工艺对其吸声系数的影响研究

通过变化麦秸和聚氨酯的比例、麦秸/聚氨酯复合人造板的密度、以及发泡温度,研究了麦秸/聚氨酯复合人造板在不同的声波频率下的吸声系数。试验结果表明:在不同的声波频率下,发泡温度的高低对麦秸/聚氨酯复合人造板的吸声系数有不同的影响;麦秸/聚氨酯复合人造板的密度与其吸声系数的大小关系密切;在声波频率为250～4 000Hz区域内,麦秸和聚氨酯的比例对麦秸/聚氨酯复合人造板的吸声系数影响较大。     

Biomass production of Eucalyptus boundary plantations and their effect on crop productivity on Ethiopian highland Vertisols

In recent years, Eucalyptus globulus planted along field boundaries has come to dominate the central highland landscape of Ethiopia. Although evidence is scanty, there is a perception that this practice adversely affects crop productivity. An on-farm trial was conducted on Pellic Vertisol at Ginchi to determine the production potential of eucalypt boundaries and their effect on the productivity of adjacent crops of tef (Eragrostis tef) and wheat (Triticum sp.). The experiment comprised three stand ages, four field aspects and six distances from the tree-crop interface, using a split-split plot design with three replicates. Wood production rates ranged between 168 kg ha⁻¹ y⁻¹ (four years old) and 2901 kg ha⁻¹ y⁻¹ (twelve years). Thus eucalypt boundaries planted on a hectare of land would satisfy 50 to 75% of the annual biomass energy requirement of a rural household of five persons. Significant depression of tef and wheat yields occurred over the first 12m from the tree line: the reduction was 20 to 73% for tef and 20 to 51% for wheat, equivalent to yield losses of 4.4 to 26% and 4.5 to 10% per hectare respectively. Nevertheless, in financial terms, the tree component adequately compensated for crop yield reduction and even generated additional income. Therefore, eucalypt boundaries have great potential to satisfy the rising demand for wood, without requiring a major change in land use on the highland Vertisols. The greater availability of wood will reduce the demand for dung and crop residues for fuel, and thus may contribute to improved soil management on croplands while relieving the increasing pressure on indigenous forest and woodlands.