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本研究以一年生裸果木幼苗为材料,采用盆栽育苗方式,设计7个不同浓度NaCl溶液(CK、0.2%、0.4%、0.6%、0.8%、1.0% 和1.2%)模拟盐分胁迫,测定分析了其叶片气体交换参数、光响应曲线及叶绿素荧光参数的变化规律。结果表明:随着盐浓度的增加,叶片净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)均呈下降趋势,NaCl浓度≥0.4%时,各处理Pn、Gs和Tr均显著低于CK;气孔限制值(Ls)和瞬时水分利用效率(WUE)随盐浓度增加呈先升后降趋势,胞间CO2浓度(Ci)呈相反趋势,NaCl浓度为0.4%时,Ls达到最大值,而Ci达到最小值,说明Pn下降以气孔限制因素为主,而当NaCl浓度≥0.6%时,以非气孔限制为主要因素。随着盐胁迫程度的增大,最大净光合速率(Pnmax)、暗呼吸速率(Rd)、光饱和点(LSP)、表观量子效率(AQY)逐渐降低,光补偿点(LCP)逐渐增加,表明盐分抑制了幼苗对光的吸收、利用和转换能力。叶片PSII潜在活性(Fv/F0)、原初光能转化效率(Fv/Fm)、实际光化学效率(ΦPSII)、电子传递速率(ETR)和光化学猝灭系数(qp)随着盐浓度的增加呈下降趋势;非光化学猝灭系数(NPQ)在≤0.4%NaCl处理下较CK显著增加,盐浓度≥0.8%时,NPQ显著下降。基质的NaCl浓度在0.2%和0.4%时,裸果木叶片Pn、Fv/F0、Fv/Fm下降不显著,WUE有所提高,PSII系统可以通过耗散过剩的光能保护光合机构,表现出一定的耐盐性;但盐浓度超过0.6%时,光合生态幅变窄,光合机构受到明显破坏,显著抑制了光合作用能力。 相似文献
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张婷袁伟涛郭肖兰王凯英徐超 《动物营养学报》2023,(4):2571-2579
This experiment was conducted to study the effects of dietary lactic acid bacteria (LAB) and fructooligosaccharides (FOS) on growth performance, nutrient digestibility and serum biochemical indices of Arctic foxes (Alopex lagopus) during early growth period. Sixty male post-litter Arctic foxes with the average body weight of (1.16±0.15) kg and at the age of (45±3) days were randomly assigned to 4 groups with 3 replicates per group and 5 foxes per replicate. The foxes were fed the following diets, respectively: basal diet (control group), basal diet+3×109 CFU / kg LAB (LAB group), basal diet+5 g / kg FOS (FOS group) and basal diet+3×109 CFU / kg LAB+5 g / kg FOS (LAB+FOS group). The experiment lasted for 30 days. The results showed as follows: 1) on the days 1 to 15, the diarrhea rate of foxes in the LAB group and LAB+FOS group was significantly lower than that in the control group and FOS group (P<0.05). On the days 16 to 30, the average daily gain of foxes in the LAB+FOS group was significantly higher than that in the control group and FOS group (P<0.05), but had no significant difference with LAB group (P>0.05); the diarrhea rate of foxes in the LAB+FOS group was significantly lower than that in the control group and FOS group (P<0.05), but had no significant difference with LAB group (P>0.05); compared with the FOS group, the feed to gain ratio of foxes in the LAB+FOS group was significantly decreased (P<0.05). 2) The dry matter digestibility of foxes in the LAB + FOS group was significantly higher than that in the control group and FOS group (P < 0.05), but had no significant difference with LAB group (P>0.05). There were no significant differences in nitrogen intake, fecal nitrogen, urine nitrogen, nitrogen deposition, net protein utilization and protein biological value among all groups (P>0.05). 3) Serum total protein (TP) content of foxes in the LAB+FOS group was significantly higher than that in the FOS group (P<0.05), but had no significant difference with control group and LAB group (P>0.05). Serum albumin (ALB) content of foxes in the LAB group was significantly higher than that in the control group and FOS group (P<0.05), but had no significant difference with LAB+ FOS group (P>0.05). Serum urea nitrogen (UN) content of foxes in the LAB+FOS group was significantly decreased compared with the control group (P < 0. 05). In conclusion, the combined supplementation of 3 × 109 CFU / kg LAB and 5 g / kg FOS in the diet is beneficial to reduce diarrhea rate, improve dry matter digestibility, improve body immune level, and promote the growth of Arctic foxes during early growth period. © 2023 Chinese Journal of Urology. All rights reserved. 相似文献
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Fat is an essential element for animal.The paper reviewed recent research progress on fat nutrition in monogastric animal at home and abroad, combining with the fat digestion and absorbing form in animal body, and summarized biological function and application of fat in production, so as to provide theoretical basis for better utilization of fat in animal production and exploration its mechanism of action. 相似文献
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<正>曾经全靠人工的农活儿,在机械、新技术的助力下,逐渐变得轻松。如今,智慧农业已不再是梦想。机械做记录农产品插上翅膀西红柿不是长在土里,而是在水里培养;稻田里,植保无人机在空中盘旋喷药;温室大棚的光线能自动调整,果蔬需要快速生长时光线直射,需要慢生长时则可斜射;扫二维码就能查到农产品的种子来源、施肥、用药情况……以上这一切不是未来,是山西已经实现了的智 相似文献
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ie0基因是家蚕核型多角体病毒(BmNPV)的一个晚期基因,该基因编码氨基酸序列的高度保守性及多种结构域提示其在病毒感染过程中的重要性。利用Red同源重组技术,分别对BmNPV ie0基因编码不同结构域的序列以及完整序列进行敲除构建ie0缺失型病毒,以野生型病毒为对照,研究ie0序列完全或部分缺失对病毒转录、复制及对宿主细胞致病能力等方面的影响。结果表明ie0基因完整序列或编码各结构域的序列缺失后均会对病毒产生不同影响:宿主细胞中完全缺失型和部分缺失型病毒滴度均有不同程度降低;完全缺失型和部分缺失型病毒基因组的拷贝数均与野生型病毒无显著差异;部分缺失型病毒的晚期和极晚期基因转录水平变化与ie0基因完全缺失型病毒相一致,均显著低于野生型病毒,并且缺失锌指结构域编码序列的影响最为明显。MTT检测结果也显示ie0基因完全缺失后病毒对宿主细胞的致病能力有所下降。亚细胞定位显示IE0蛋白在细胞质、细胞核中均有分布,但主要位于细胞质。综上所述,ie0基因为BmNPV病毒增殖、基因组复制的非必需基因,但对病毒晚期和极晚期基因的转录具有调控作用,影响最为显著的为锌指结构域的编码序列,且该基因的缺失会导致病毒对宿主细胞的致病能力有所下降。 相似文献
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