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寒地早粳花培培养基中铁的效应(英文) 总被引:1,自引:0,他引:1
In this study, through vitro culturing anthers of 7 F1 progenies of early Japonica rice in cold region on medium with different Fe2+ contents, it was found that Fe2+ content generated greater impacts on the induction rate and green plantlet differentiation. The result demonstrated that if Fe2+ increased from 32 to 40 mg/kg, the induction rate of early Japonica rice anther culture in N6 culture media was more then 1.4 times higher than that in N6 culture media containing 5.6 mg/kg Fe2+. In this concentration range, the induction rate increased with the increase of Fe2+ content, while if the concentration was over this concentration range, the induction rate decreased with the increase of Fe2+, showing single peak distribution. When the Fe2+ was 40 mg/kg in differentiation medium, the differentiation rate decreased dramatically. The green plantlet differentiations of callus which were induced on culture media containing 32-40 mg/kg Fe2+ were different, when they were cultured on MS culture media, and 85.7% materials could increase green plantlet productivity to about 7.8%. Therefore, increasing Fe2+in induction media properly could increase anther culture efficiency of early Japonica rice in cold region. 相似文献
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水稻新品系龙选948龙选则是黑龙江省农科院水稻所,于1987年以龙交82133(松前/雄基9号的F5)为母本,以N193-2为父本杂交;1988年接种其F1代花药,经离体培养而育成的早熟优质花培水稻新品系。1995年提升全省第三积温带参加区域试验。1…… 相似文献
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寒地早粳花培育种研究进展 总被引:7,自引:3,他引:7
在花药培养技术上,通过49个组合400个幼穗1200个颖花的研究,提出了依据植株和颖花形态判断花粉发育时期的具体指标;通过低温预处理和双态双层法提高花药力的方法。在花培育种遗传规律的研究上,对株高和叶瘟抗性的结果表明:F2的分布倾向于晚熟、高秆及抗病,即倾向于亲本的显性性状,而H2的分布则均匀对称,其中早熟、矮秆及感病植株的比例较F2高。在选择效率上,花培育成的审定品种的选择效率为0.93%,是同期常规育成审定品种选择效率0.28%的3.32倍。通过上述研究培育出了8个花培新品种,并且在品质、抗性、产量等方面表现突出,成为寒地稻区主栽品种,并讨论了水稻花培育种今后的发展方向。 相似文献
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双态双层培养法提高粳稻花药培养力的研究 总被引:3,自引:2,他引:1
通过对7个杂交组合F1代和1个品系的花药培养,初步明确了:①培养基琼脂含量影响粳稻花药愈伤组织诱导率;②液体培养的花药愈伤组织诱导率是固体培养的6.1倍,但绿苗分化度比固体培养低2.7倍;③双态双层培养可以提高粳稻花药培养力,使其达到了10.4%-22.7%,是固体培养的2.2倍;④使用30mm×200mm试管,以0.9-1.1ml液膜注入量对提高花药培养力效果明显,是对照的1.8-2.1倍;⑤愈 相似文献
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