安徽省知母药材质量研究

[目的]研究安徽产知母药材中知母皂苷BⅡ和芒果苷的含量.[方法]知母皂苷BⅡ采用Diamonsil C18柱（250 mm ×4.6 mm,5μm）、乙腈-水等度洗脱（25∶75）,流速1.0 ml/min、ELSD检测器漂移管温度60℃、载气流速3.0 ml/min、柱温为室温（25℃）;芒果苷采用Diamonsil C18柱（150 mm×4.6 mm,5μm）、乙腈-0.2％冰醋酸水溶液（15∶85）等度洗脱,流速1.0 ml/min.[结果]知母皂苷BⅡ在1.036～10.360 μg范围内（r=0.999 6）进样量的对数值与峰面积的对数值呈良好的线性关系,芒果苷在0.05 ～1.00 μg范围内（r=0.9994）进样量与峰面积呈良好的线性;知母皂苷BⅡ平均回收率为99.7％,RSD=1.19％;芒果苷平均回收率为100.3％,RSD=1.42％.[结论]安徽省知母药材中知母皂苷BⅡ与芒果苷含量均符合2010版药典含量标准.     

基于ARM9内核的智能灌溉控制系统的设计

介绍了基于ARM920T内核的嵌入式控制系统的设计与实施,并将控制系统引入设施栽培自动灌溉控制中。实践证明,该系统的设计达到了预期的效果。     

反刍家畜主组织相容复合物的研究进展

MHC是由紧密连锁的高度多态的基因座位所组成的染色体上的一个遗传区域 ,由于在免疫系统中发挥着重要作用并与抗病性能密切相关 ,因此近年来成为家畜抗病育种研究中最为活跃的部分。本文综述了牛、绵羊和山羊MHC的结构、特点及其与抗病性状之间的关系 ,同时讨论了MHC在家畜育种中的应用前景。     

Protective effect of adiponectin on glucose and lipid metabolism by suppressing MHCⅡ expression

AIM: To investigate whether the protective effect of adiponectin on glucose and lipid metabolism is achieved through down-regulating major histocompatibility complex class Ⅱ (MHCⅡ) in the adipose tissue. METHODS: Adiponectin knockout (KO) mice and C57BL/6(WT) mice were fed with high-fat diet and standard diet for 24 weeks, respectively. The body weight, fasting blood glucose (FBG), fasting insulin (FINS), homeostasis model assessment of insulin resistance (HOMA-IR), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), hepatic histology, and class Ⅱ trans-activator (CⅡTA), histocompatibility 2 class Ⅱ antigen E beta (H2-Eb1) and cluster of differentiation 74(CD74) mRNA and MHC Ⅱ protein levels in adipose tissue were measured at sacrifice. siRNA targeting MHC Ⅱ and overexpression vector was used in 3T3-L1 cells to explore the effect of adiponectin on the protein level of MHCⅡ. RESULTS: The levels of body weight, FBG, FINS, HOMA-IR, TC, TG, LDL-C, hepatic steatosis, CⅡTA, H2-Eb1 and CD74 mRNA expression, and MHCⅡ protein expression in the KO mice were higher than those in the WT mice that fed with high-fat diet or standard diet. In 3T3-L1 cells, inhibition of adiponectin reversed MHC Ⅱ protein level induced by specific siRNA. The expression of MHC Ⅱ in adipocytes decreased after adiponectin was overexpressed. CONCLUSION: Adiponectin improves glucose and lipid metabolism through suppressing the expression of MHCⅡ in the adipose tissue.     

玉米几个主要农艺性状的遗传研究

采用增广NCⅡ设计,研究玉米的株高、穗位高、穗长、穗粗、穗行数、粒长、百粒重和小区产量的遗传。结果表明,穗位高和百粒重符合加性-显性遗传模型,株高和穗行数除加性-显性效应外还有上位性效应,粒长和小区产量除加性效应外还有上位性效应,穗粗和穗长只有加性效应。显性性质:株高和穗位高平均为部分显性,百粒重和穗行数平均为超显性,显性方向株高、穗位高、百粒重和穗行数均为双向显性。     

玉米CMS-S恢复基因Rf3近等基因系的蛋白质分析

 【目的】Rf3是玉米CMS-S型不育系两个以上恢复基因中的标准基因，对其进行分子生物学研究意义很大。【方法】利用自主创建的Rf3近等基因系不育群体（Ps）和可育恢复系群体（Pf）为试材，采用改进的双向（二维）聚丙烯酰胺凝胶电泳（2D-PAGE）技术，对苗期、成株期叶片细胞总蛋白进行分析。【结果】分离到在Pf遗传背景下分子量为25.2 kD，等电点为5.0的特异蛋白质RRPⅡ，优化了2D-PAGE技术。【结论】该特异蛋白质点的出现与消失可能与玉米CMS-S的育性恢复有一定关系，对其纯化分离将为Rf3基因的克隆奠定基础和进一步了解玉米质核互作不育表达模式的分子机制。结合本研究，文章还讨论了采用2D-PAGE技术在玉米分子生物学研究中的优化以及基因组学与蛋白组学研究的衔接。     

质粒DNA 免疫小鼠制备IGF-Ⅱ抗体的研究

[目的]用基因免疫法制备人类胰岛素样生长因子Ⅱ(IGF-Ⅱ)的抗体,从而检验基因免疫法的可行性。[方法]通过RT-PCR法克隆到IGF-Ⅱ基因,将其连接到pcDNA3.1A载体上,用含IGF-Ⅱ基因的质粒通过皮下、肌肉注射2种途径免疫小鼠,制备抗血清;用Western blot法检测抗体特异性和抗体效价,探索基因免疫法制备抗体的方法,并进一步观察免疫次数及途径对抗体产生的影响。[结果]基因免疫不同次数的小鼠体内均有抗体产生,且皮下、肌肉不同免疫途径的血清均显示阳性结果,其中皮下注射免疫抗体产生较早;抗体效价可达到1∶500,而且特异性较强。[结论]通过基因免疫法制备IGF-Ⅱ抗体是可行的,而且效果较好。     

“防僵灵Ⅱ号”对家蚕真菌病的防治效果研究

[目的]有效控制家蚕真菌病的发生与蔓延。[方法]研究蚕药"防僵灵Ⅱ号"对家蚕真菌病的防治效果和对家蚕的生长发育、全茧量、茧层量的影响。[结果"]防僵灵Ⅱ号"在稀释500倍以上时对家蚕常见真菌病的防治效果达100%,对家蚕无明显毒副作用。[结论]"防僵灵Ⅱ号"对于家蚕真菌病具有良好的防治效果,同时是一种对家蚕安全的药剂。     

Effects of puerarin on apelin-12, angiotensin II,NO and blood pressure in renovascular hypertensive rat

AIM: To investigate the effects of puerarin on blood pressure in renovascular hypertensive rats, and to measure puerarin-induced changes of apelin-12, angiotensin II (Ang II) and nitric oxide(NO), the factors related to development of hypertension. METHODS: Sixty-five male Sprague-Dawley rats were used, of which 8 rats were randomly selected as sham operation group, and the remaining were used to make two-kidney, one-clip model. The rats that met the criterion for Goldblatt hypertensive rat model were randomly allocated into 5 groups: high-, middle- and low-dose puerarin groups, captopril group, and model group. The drugs were administered for 6 weeks. Blood pressure was measured every 2 weeks. Six weeks after treatment, all rats were sacrificed under deep anesthesia. Blood and kidney samples were collected. The level of apelin-12 in serum and kidneys was detected by ELISA. The level of Ang II in plasma and kidneys was measured by radioimmunoassay. NO level in serum was examined by nitrate reductase assay. RESULTS: Puerarin had an antihypertensive effect in a dose-dependent manner. Marked decreases in the level of serum apelin-12 in high- and middle-dose puerarin groups were observed(P<0.01). Puerarin at low dose did not cause obvious change in the content of apelin but still reached significant level (P<0.05). As the dose of puerarin went up, the level of apelin-12 in the kidneys was gradually decreased. Puerarin at high and middle doses obviously reduced the level of AngII in plasma, while purarin at low dose did not produce any significant effects. Puerarin at high and middle doses markedly increased the level of NO in serum, but puerarin at low dose did not induce any significant changes. CONCLUSION: Puerarin has an antihypertensive effect, and its mechanism may be related to inducing the changes of apelin, Ang II and NO, and regulating the balance among those factors.     

棉花早熟芽黄突变体叶绿素荧光动力学特性研究

以中棉所58及其航天诱变芽黄突变体为研究对象,利用快速叶绿素荧光诱导动力学测定和JIP-test数据分析方法,研究了晴天条件下野生型(Wild type,WT)和突变体5个叶位叶片(倒1叶至倒5叶)的原初光化学反应的变化.结果表明,突变体倒2叶最大光化学效率(Fv/Fm)最低,至倒5叶时恢复正常.突变体叶片较高的K点的...