Sex‐dependent responses of perch to changes in water clarity and temperature

Rising temperatures and decreasing water transparency of lakes have strong wide ranging effects on fish. Fish responses to various changes in the environment are usually species‐dependent, but responses may also vary within species. In general, large individuals are considered to be more sensitive to environmental variation due to higher energy demand, than smaller individuals. Similarly, large individuals require more food to maintain bodily functions and are thus more sensitive to resource and food scarcity. These size‐specific responses to environmental gradients are also sex‐dependent in species that exhibit sexual size dimorphism (SSD). We studied in enclosures with short‐term experiments how rising temperatures and decreasing water transparency regulate the feeding rates of female and male European perch (Perca fluviatilis L.). To explore experimental results, we calculated perch SSD in nine lakes with varying environmental conditions using previously collected field data. The results of the experiments revealed that the combined effect of water transparency and temperature on the feeding rate of fish is gender‐dependent: feeding rate of females decreased more than that of males. The experimental results were also supported by field data that revealed a negative relation between water transparency and the magnitude of SSD in perch. Our results suggest that rising temperatures and decreasing water transparency may potentially decrease fish size in a sex‐dependent manner. As female size is one of the main demographic traits determining the reproductive success of a fish population, changing environments may have unexpected and far‐reaching consequences on fish population dynamics.     

Comparison of Chemical Composition and Biological Properties of Essential Oils Obtained by Hydrodistillation and Steam Distillation of Laurus nobilis L.

Plant Foods for Human Nutrition - The purpose of this study was to compare the yield, chemical composition, antimicrobial and antioxidant properties of essential oils isolated from leaves of Laurus...     

Effect of fish meal replacement with Carinata Brassica carinata in low animal protein diets of rainbow trout Oncorhynchus mykiss (Walbaum) on trypsin activity,protein and amino acid digestibility and bioavailability

Replacement of >25% of fish meal (FM) with aerobically converted carinata meal (ACCM) in low (200g/kg) animal protein (reference = 200g/kg FM) diets of rainbow trout Oncorhynchus mykiss resulted in reduced growth, partly due to reduced feed consumption and protein utilization. In this study, we determined the effect of FM replacement with ACCM on trypsin activity, apparent digestibility coefficients (ADCs) of protein, and essential amino acid (EAA) or conditionally essential amino acid (CEAA) ADCs and bioavailability. Replacement of FM did not alter protein ADCs or trypsin activity. Replacement of >25% FM reduced all EAA and CEAA ADCs except for arginine and leucine which were only reduced by 75% FM replacement. Only serum free lysine and muscle free histidine were reduced by >25% FM replacement. Muscle free lysine was only reduced by 75% FM replacement. Replacement of FM reduced EAA peak concentrations and resulted in slower release of EAAs in serum. Cumulative total EAAs in serum and muscle decreased with FM replacement. Ratios of EAAs to lysine showed that tryptophan was the most limiting EAA. However, isoleucine, leucine, methionine and phenylalanine were also inadequate for muscle synthesis for the first 9–12 hr following force‐feeding. Optimal time for protein synthesis was ≥36 hr. Although any level of FM replacement did not reduce protein ADCs and trypsin activity, replacement of ≥25% FM reduced EAA ADCs and bioavailability of lysine and histidine, which partly contributed to the observed differences in growth.     

Climate effects and bottom‐up controls on growth and size‐at‐age of Pacific halibut (Hippoglossus stenolepis) in Alaska (USA)

Pacific halibut (Hippoglossus stenolepis) are an ecologically, commercially, and culturally important Alaskan groundfish species. Commercial harvest of halibut dates back to the late 19th century and has been managed by the International Pacific Halibut Commission (IPHC) since 1921. IPHC surveys have revealed declining trends in survey biomass in multiple regions and region‐specific declines in mean size‐at‐age (size‐at‐age) over the past two decades (>50% in some areas). Changes in size‐at‐age can arise from a variety of physical, ecological, sampling, and fishery effects, including size‐dependent fishery or predation mortality, alteration in growth from variability in prey quality or quantity, and changes in temperature‐dependent metabolic demands. Here, we develop and apply a bioenergetics model for halibut using survey‐based diet and temperature data for Alaska to evaluate potential environmental drivers of size‐at‐age. In general, juvenile (<40 cm fork length) foraging rates were highest in the Gulf of Alaska concomitant with higher potential growth and elevated basal metabolic demands during warm summer conditions. In contrast, adult (40–120 cm FL) potential growth was highest in the Eastern Bering Sea, potentially reflecting lower metabolic costs and higher rates of prey consumption in that region. We additionally find evidence for interannual variation in potential growth, with a higher frequency of reduced growth potential in the last decade, particularly in the Eastern Bering Sea in 2015 and 2016 for both juvenile and adult halibut. These results suggest the potential for patterns in size‐at‐age to arise from trophic and environmental constraints that collectively limit growth in some regions and years.     

Comparative ecologies of domestic and naturalised rainbow trout in northern Lake Huron

Northern Lake Huron (Ontario, Canada) supports the largest concentration of freshwater salmonid cage culture farms in Canada and receives inputs of domestic‐strain rainbow trout (Oncorhynchus mykiss) through escapement. We assessed the potential effects of these domestic fish on the naturalised rainbow trout of this ecosystem by comparing their respective ecologies. Mature adults were sampled from spawning tributaries, primarily in spring, and to a lesser extent in fall. Fish of domestic origin comprised ~80% of rainbow trout sampled from sites near cage culture facilities but <20% of rainbow trout sampled from more distant sites. Domestic adults in spawning condition (gametes free‐flowing) were present in the spawning tributaries in both spring and fall sampling seasons, whereas naturalised fish in spawning condition were only observed in the spring. Domestic adults were younger and smaller (in length), appeared to have shorter lifespan and had a higher male to female ratio compared with their naturalised counterparts. Growth rates (change in length with age) of naturalised and domestic females in the wild were similar, but domestic males grew more slowly than naturalised males. Domestic females also produced smaller eggs than naturalised females. Food web positions (inferred from δ¹³C and δ¹⁵N) of domestic and naturalised fish were very similar but varied more strongly with body size in the former. Domestic‐strain rainbow trout of cage culture origin can survive, grow and attempt to spawn in northern Lake Huron and have the potential to compete for food, mates and spawning habitat with naturalised rainbow trout.     

Remote sensing of biotic and abiotic stress for irrigation management of cotton

The applicability of commercially available remote sensing instrumentation was evaluated for site-specific management of abiotic and biotic stress on cotton (Gossypium hirsutum L.) grown under a center pivot low energy precision application (LEPA) irrigation system. This study was conducted in a field where three irrigation regimes (100%, 75%, and 50% ET_c) were imposed on areas of Phymatotrichum (root rot) with the specific objectives to (1) examine commercial remote sensing instrumentation for locating areas showing biotic and abiotic stress symptomology in a cotton field, (2) compare data obtained from commercial aerial infrared photography to that collected by infrared transducers (IRTs) mounted on a center pivot, (3) evaluate canopy temperature changes between irrigation regimes and their relationship to lint yield with IRTs and/or IR photography, and (4) explore the use of deficit irrigation and the use of crop coefficients for irrigation scheduling. Pivot-mounted IRTs and an IR camera were able to differentiate water stress among irrigation regimes. The IR camera distinguished between biotic (root rot) and abiotic (drought) stress with the assistance of groundtruthing. The 50% ET_c regime had significantly higher canopy temperatures than the other two regimes, which was reflected in significantly lower lint yields when compared to the 75% and 100% ET_c regimes. Deficit irrigation down to 75% ET_c had no impact on lint yield, indicating that water savings were possible without reducing yield.     

Soil variability through spatial scales in a permanently disturbed natural spruce-fir-beech forest

Soil variability was assessed in a 74.2-ha area within the Žofínsky prales natural forest. Parameters evaluated for 1765 soil profiles inside 353 graticule plots were as follows: (1) thickness of organic horizons, (2) thickness and form of mineral horizons, (3) humus form (HF), (4) soil taxonomic unit (STU) and (5) anomalies. In addition, soil reaction (pH_KCl) and oxidizable carbon content (C_ox) were measured in the laboratory for 734 samples from the upper mineral (A) and lower mineral (B) horizons. The most frequently occurring humus form was mor followed by moder, hydromor and peaty T-horizon. Entic Podzols, Dystric Cambisols, Haplic Cambisols, Albic Podzols, Histic (or Haplic) Gleysols, Endogleyic Stagnosols, Fibric or Hemic or Sapric Histosols and Stagnic Gleysols were all present at the site despite its homogeneous geological bedrock. Overall coefficient of variance (CV) was lower in terrestrial soils compared with (semi-)hydromorphic soils. Overall variance decreased in both soil groups with increasing depth, as did CV differences between the fine (up to 10 m) and the locality scales. The lowest CV values occurred for C_ox and pH_KCl. The CV values differed between STUs as well. Compared to lower horizons, variograms of upper horizons showed greater autocorrelation at the intermediate spatial scale (10–320 m)—ranging from 50 to 150 m. Semivariance values, however, reached 70–80% of sill already at a distance of 10 m. The most significant factor of variability at all studied spatial scales is presumably the soil disturbance regime, followed by terrain micro-topography and the effect of tree species.     

Screening sorghum genotypes for salinity tolerant biomass production

Genetic improvement of salt tolerance is of high importance due to the extent and the constant increase in salt affected areas. Sorghum [Sorghum bicolor (L.) Moench] has been considered relatively more salt tolerant than maize and has the potential as a grain and fodder crop for salt affected areas. One hundred sorghum genotypes were screened for salinity tolerance in pots containing Alfisol and initially irrigated with a 250-mM NaCl solution in a randomized block design with three replications. Subsequently 46 selected genotypes were assessed in a second trial to confirm their responses to salinity. Substantial variation in shoot biomass ratio was identified among the genotypes. The performance of genotypes was consistent across experiments. Seven salinity tolerant and ten salinity sensitive genotypes are reported. Relative shoot lengths of seedlings were genetically correlated to the shoot biomass ratios at all stages of sampling though the relationships were not close enough to use the trait as a selection criterion. In general, the whole-plant tolerance to salinity resulted in reduced shoot Na⁺ concentration. The K⁺/Na⁺ and Ca²⁺/Na⁺ ratios were also positively related to tolerance but with a lesser r ². Therefore, it is concluded that genotypic diversity exists for salt tolerance biomass production and that Na⁺ exclusion from the shoot may be a major mechanism involved in that tolerance.     

Characterization of nematode colonization and succession in disturbed soil using the Maturity Index

Nematode colonization and succession were studied over 60 weeks in field plots which had been disturbed by combinations of fumigation and manuring. It was expected that the Maturity Index, which is based on the composition of the nematode fauna, would decrease after disturbance, and subsequently increase with recovery and succession. The results showed that in all treatments, the initial colonization and growth were due to taxa scaled in colonizer-persister (c-p) group 1, resulting in a low Maturity Index. An increase in this index followed when these taxa were gradually replaced by c-p group 2 which thereafter remained the dominant group. C-p groups 3–5 developed only in the non-fumigated treatment. Manuring caused characteristic changes in the nematode fauna which could be described as a cycle, starting with an increase in enrichment opportunists (c-p 1), which were succeeded by general opportunists (c-p 2) which, in turn, were supplemented with persisters (c-p 3–5). It is expected that the same cycle will be observed as a result of other disturbances that cause temporary increase in food supply.     

Safety evaluation of an oak-flavored milk powder containing ellagitannins upon oral administration in the rat

Ellagitannins are phenolic compounds that occur in a number of dietary sources such as walnuts, raspberries, pomegranate, strawberries, and oak-aged wines. Ellagitannins have been acknowledged to have antioxidant and cancer chemopreventive activities. However, ellagitannins have also been considered to be "antinutrients" and have been reported to provoke adverse effects in animals. In this context, risk assessment studies are demanded to ensure that chemicals present in food do not compromise food safety. Oak-flavored milk can be obtained by thermal treatment of milk in the presence of oak shavings. The oak-flavored milk can be used to impart wood-like flavor to different recipes. The present study evaluated, under a subchronic approach, the safety of an oak-flavored milk powder containing ellagitannins in rats upon repeated oral administration for 96 days. The effect of 30% oak-flavored milk powder in the diet was evaluated. This amount involved a margin of safety equivalent to the daily intake of 134 yogurts by a 70 kg person for 96 days. Growth rate, food utility index, 9 hematological, and 13 serobiochemical parameters as well as the metabolism and tissue distribution of ellagitannins and derived metabolites were studied. Overall, no significant differences were found in oak-milk-fed rats in any blood parameter analyzed. No effect was found on growth rate and food utility index. Urolithin A was identified in feces of rats fed oak-flavored milk and the glucuronide derivative in urine. No metabolites were found in the liver, kidney, or uterus. Histopathological analysis of liver and kidney corroborated the absence of toxicity. Therefore, these results suggest that the intake of the oak-flavored milk powder assayed in this study is safe and does not involve potential hazard to human health.