Chemical compositions and insecticidal effects of essential oils isolated from Achillea gypsicola, Satureja hortensis, Origanum acutidens and Hypericum scabrum against broadbean weevil (Bruchus dentipes)

The essential oils of aerial parts of Achillea gypsicola Hub.-Mor., Hypericum scabrum L., Satureja hortensis L., and Origanum acutidens (Hand.-Mazz.) Letswaart were analyzed in this study by GC and GC–MS and their oils were tested for toxicity against broadbean weevil (Bruchus dentipes). A. gypsicola oil contained camphor (40.17%), 1,8-cineole (22.01%), piperitone (11.29%), borneol (9.50%) and α-terpineol (1.56%) as major components. A total of 74 components were identified by GC–MS in H. scabrum oil, including α-pinene (9.26%), terpinen-4-ol (5.12%), camphor (5.94%), δ-cadinene (4.52%), pulegone (4.45%), γ-muurolene (4.12%), pinocarvone (3.97%) and β-caryophyllene (3.42%) as predominant components. The essential oils of O. acutidens and S. hortensis were characterized by high contents of carvacrol (86.99% and 55.74%), γ-terpinene (0.71% and 20.94%), p-cymene (1.95% and 12.30%), α-terpinene (0.13% and 2.04%) and β-caryophyllene (1.30% and 1.08%). All of the essential oils were toxic to adults of B. dentipes and insect mortality increased with increasing concentration of each oil. The oils (20 μl dose) brought about 100% mortality in 36 h. Although desirable insecticidal activities against the pest were achieved with the oils from all four plant species, S. hortensis and O. acutidens oils were more effective, particularly after 6 h of treatment. The current results concluded that the essential oils, in particular O. acutidens and S. hortensis oils, may be used as potential botanical insecticides against B. dentipes.     

Hemorrhagic syndrome-like disease in calves with bovine viral diarrhea and mucosal disease complex

BACKGROUND: Bovine viral diarrhea virus (BVDV) infection is one of the causes of hemorrhagic diathesis in cattle but there have been limited field studies about that condition. HYPOTHESIS: To identify the cause of hemorrhagic diathesis in calves and describe its clinical findings. ANIMALS: Five calves from a farm with 150 dairy cows. METHODS: Clinical examination of the calves was performed. After blood samples were obtained from 2 calves, whole blood, sera, and leukocyte samples were used for hematologic and hemostatic examinations, neutralization tests, virus isolation, and viral genome sequencing. RESULTS: The calves had moderate pyrexia, dullness, serous or mucous nasal discharge, and petechial and ecchymotic hemorrhages on mucosal surfaces. Severe thrombocytopenia and anemia were identified on hematologic examinations. All calves died within 10 days of the onset of clinical signs. Virologic examinations identified BVDV as the causative agent of the disease. CONCLUSIONS AND CLINICAL IMPORTANCE: This paper identifies a hemorrhagic syndrome-like disease in calves with bovine viral diarrhea and mucosal disease complex in Turkey.     

Functional characteristics of the growth factor receptor family and some ligands in the oropharyngeal cavity of the Chukar partridge (Alectoris chukar)

1. The aim of the present study is to describe, immunohistochemically, the expression and cell type localisation of growth factor receptors and some of their ligands in the oropharyngeal organs of the Chukar partridge.

2. The tissue samples from 10 healthy adult partridges were dissected under ether anaesthesia and then embedded in paraffin following routine histological procedures. The immunoreaction for receptors and ligands of the epidermal growth factor receptor (EGFR)/ligand system was localised in the cell membrane, nucleus and cytoplasm of the luminal and glandular epithelial cells, stromal and striated muscle cells, and vascular endothelial and smooth muscle cells.

3. Variations were observed in the avian oropharyngeal organs. The immunostaining for the erbB1/HER1 (human epidermal growth factor receptor 1) and the EGF (epidermal growth factor) and AREG (Amphiregulin) ligands in the luminal epithelial cells was higher than in the glandular epithelial, stromal and striated muscle cells. However, the immunostaining for erbB3/HER3 (human epidermal growth factor receptor 3) and erbB4/HER4 (human epidermal growth factor receptor 4) were similar in the luminal epithelium, stromal and striated muscle cells.

4. Growth factor receptors and some of their ligands were localised in different cell types in the oropharyngeal organs. We suggest that erbB/HERs (human epidermal growth factor receptors) and their ligands play an important role in proliferation, differentiation, growth, survival and migration of the cells.     

Genetic diversity assessments of brown rot pathogen Monilinia fructicola based on the six simple sequence repeat loci

Journal of Plant Diseases and Protection - Monilinia fructicola, causal agent of brown rot of stone fruits, is an economically important problem worldwide. Six of the sequence tagged microsatellite...     

Clonal variation in chemical wood characteristics in Hanönü (Kastamonu) Günlüburun black pine (Pinus nigra Arnold. subsp. Pallasiana (Lamb.) Holmboe) seed orchard

ABSTRACT

Scientific evidence suggests forest cover across the world is gradually decreasing while the world’s population is rapidly increasing. In line with this, the demand on raw wood is also increasing, making it impossible for the already limited natural resources to meet human needs in proportion to the increasing population size. To counter this, there is a need to increase the variety and availability of timber through genetic improvement. This has been seen in genetic improvement studies in forestry which have resulted in seed orchard establishments; however, the genetic variation in these seed orchards continues to be a topic of debate. This study aims to determine the clonal variation in chemical characteristics in a Hanönü (Kastamonu) Günlüburun black pine (Pinus nigra Arnold. subsp. pallasiana (Lamb.) Holmboe) seed orchard. The analysis shows differences between the clones were seen at the 99.9% confidence level. Ash content, NaOH, extractive substance, and hot water solubility exhibited the highest heritability scores, according to the heritability estimates of the clones in the seed orchard. However, hemicellulose content showed an extremely low H₂ (0.1).     

Antibodies to Schmallenberg virus in domestic livestock in Turkey

This, partly retrospective study, was designed to determine the seroprevalence of Schmallenberg virus (SBV), a new Orthobunyavirus first reported in Germany in late 2011, in domestic ruminants from the Middle Black Sea, West, and Southeast regions of Turkey. An indirect enzyme-linked immunosorbent assay was used to screen serum samples collected from slaughterhouse animals between 2006 and 2013. The overall seroprevalence was 335/1,362 (24.5 %) with 325/816 (39.8 %), 5/307 (1.6 %), 3/109 (2.8 %), and 2/130 (1.5 %) recorded in cattle, sheep, goats, and Anatolian water buffalo, respectively. This is the first study to demonstrate the presence of antibodies to SBV in Turkish ruminants; it indicates that cattle are more susceptible to infection than sheep, goats, or buffalo and that exposure of domestic ruminants to SBV in Turkey may have occurred up to 5 years prior to the first recorded outbreak of the disease in 2011.     

TEMPO-treated CNF Composites: Pulp and Matrix Effect

This study examined the effects of matrix (PVA and Si) on the properties of TEMPO-treated nanocomposites preparing from different pulp sources (Kraft and NaBH₄ treated Kraft). Chemical characterization and crystallization were determined via FT-IR, thermal stability via TGA and morphological alterations via SEM. UTM and DTMA were used to measure the Young’s and storage moduli. The real and imaginary parts of permittivity and electric modulus were evaluated using an impedance analyzer. After interaction, prominent vibrations and alteration of crystallinity were seen. Storage and Young’s moduli decreased after Si and PVA interaction. The Si-TOCN films showed higher permittivity properties and all of the films followed a similar trend of significantly dropping ε' and ε'' values at high frequency. The PVA-TOCN films had mechanical advantages at room temperature compared to the Si-TOCN films. However, the Si-TOCN films had better thermomechanical properties at high temperatures. NaBH₄ revealed favorable effects on mechanical properties of the films.     

Pre- and post-operative cardiac evaluation of dogs undergoing lobectomy and pneumonectomy

This study aimed to assess the influence of lobectomy and pneumonectomy on cardiac rhythm and on the dimensions and function of the right-side of the heart. Twelve dogs undergoing lobectomy and eight dogs undergoing pneumonectomy were evaluated preoperatively and one month postoperatively with electrocardiography and Doppler echocardiography at rest. Pulmonary artery systolic pressure (PASP) was estimated by the tricuspid regurgitation jet (TRJ) via the pulse wave Doppler velocity method. Systemic inflammatory response syndrome criteria (SIRS) were also evaluated based on the clinical and hematological findings in response to lobectomy and pneumonectomy. Following lobectomy and pneumonectomy, we predominantly detected atrial fibrillation and varying degrees of atrioventricular block (AVB). Dogs that died within seven days of the lobectomy (n = 2) or pneumonectomy (n = 1) had complete AVB. Preoperative right atrial, right ventricular, and pulmonary artery dimensions increased gradually during the 30 days (p<0.05) following pneumonectomy, but did not undergo significant changes during that same period after lobectomy. Mean PASP was 56.0 ± 4.5 mmHg in dogs having significant TRJ after pneumonectomy. Pneumonectomy, but not lobectomy, could lead to increases (p<0.01) in the SIRS score within the first day post-surgery. In brief, it is important to conduct pre- and postoperative cardiac evaluation of dogs undergoing lung resections because cardiac problems are a common postoperative complication after such surgeries. In particular, complete AVB should be considered a life-threatening complication after pneumonectomy and lobectomy. In addition, pneumonectomy appears to increase the likelihood of pulmonary hypertension development in dogs.     

Effects of Soil-Applied Materials on the Dry Weight and Boron Uptake of Maize Shoots (Zea Mays L.) Under High Boron Conditions

High concentrations of boron (B) in the soil, reduces plant growth, crops’ yield and quality. Regarding such problem, synergistic and antagonistic relations between the nutrients can be used to ameliorate the B toxicity. Therefore, a greenhouse experiment was conducted to evaluate effects of soil-applied zinc (Zn), nitrogen (N), calcium (Ca), lime (CaCO₃), potassium (K), humic acid (HA), and humus on the dry weight and B uptake of maize shoots (Zea mays L.) under high-B containing soil conditions. Increasing doses of B (0, 2.5, 5, and 10 mg kg^?1 B) were applied to soil as borax (Na₂B₄O₇10H₂O), and boric acid (H₃BO₃). Positive correlations were found between B doses and the uptake amounts (r = 0.934**; – 0.964**). However, the correlations between the dry weight and B doses (r = ?0.314**; – r = ?0.495**) and between the dry weight and the uptake amounts (r = ?0.294*; – r = ?0.497**) were negative. Among the materials, Zn and humus exhibited positive correlations with dry weight values (r = 0.249*; r = 0.525**), and an effective increase (p < 0.01) in the dry weight amounts of maize shoots was observed under toxic B conditions.     

Yield and quality response of drip irrigated broccoli (Brassica oleracea L. var. italica) under different irrigation regimes, nitrogen applications and cultivation periods

The experiment aimed at evaluating the yield and quality response of broccoli (Brassica oleracea L. var. italica) to applied irrigation water and nitrogen by drip irrigation method during the spring and autumn cultivation periods of 2007. Irrigation water was applied based on a ratio of Class A pan evaporation (k_cp = 0.50, 0.75, 1.00 and 1.25) with 7 days interval. Also, the effect of four nitrogen levels (0 kg ha⁻¹, 150 kg ha⁻¹, 200 kg ha⁻¹ and 250 kg ha⁻¹) was compared with each treatment. The seasonal evapotranspiration in the treatments varied from 233 mm to 328 mm during the spring period and from 276 mm to 344 mm during the autumn period. The highest broccoli yield was obtained in the spring period as 11.02 t ha⁻¹ and in the autumn period as 4.55 t ha⁻¹. In general, there were statistical differences along nitrogen does with respect to yield and yield components while there were no statistically significant differences in the yield and yield components among irrigation regimes. Both yield and yield parameters in the spring period were found to be higher than that of the autumn period due to the low temperature and high rainy days in autumn. Irrigation water use efficiency (IWUE) ranged from 3.78 kg m⁻³ to 14.61 kg m⁻³ during the spring period and from 1.89 kg m⁻³ to 5.93 kg m⁻³ during the autumn period. On the other hand, nitrogen use efficiency (NUE) changed as 37.32-73.13% and 13.08-22.46% for spring and autumn season, respectively.