Plant growth inhibitory activity and active substances with allelopathic potential of cogongrass (Imperata cylindrica) rhizome

Cogongrass (Imperata cylindrica [L.] Raeusch.) is a perennial rhizomatous grass that belongs to the Poaceae and is widely distributed in tropical, subtropical and temperate regions over the world. The grass is strongly invasive and is suggested to have allelopathic potential. However, limited information is available on allelopathic substances in cogongrass rhizomes. Therefore, the allelopathic potential and substances in cogongrass rhizomes were investigated. The extracts of cogongrass rhizomes inhibited the root and shoot growth of barnyard grass, ryegrass, timothy, cress, lettuce and alfalfa. After bioassay‐guided separations of the extracts, four growth inhibitory substances, 5‐methoxyflavone, 5,2′‐dimethoxyflavone, methyl caffeate and abscisic acid, were isolated. 5‐Methoxyflavone, 5,2′‐dimethoxyflavone and methyl caffeate significantly inhibited the root and shoot growth of cress at concentrations of ≥0.03–0.3 m mol L^?1 and their concentrations required for 50% growth inhibition were 0.079–0.24, 0.23–1.1 and 0.59–0.88 m mol L^?1, respectively. The other isolated substance, abscisic acid, has been reported to have strong growth inhibitory activity. Its concentrations required for 50% growth inhibition on cress were 0.31–0.61 μ mol L^?1. The present study suggests that cogongrass rhizomes might have allelopathic potential and that those four substances might contribute to that potential.     

Effects of row deep tillage for the growth base formed by piling up soil in damp lowlands behind coastal sand dunes to construct coastal disaster prevention forest belts on the Kujukuri coastline,Japan

ABSTRACT

To successfully afforest coastal forest belts ensuring high disaster prevention, growth bases for them have been constructed by piling up soil in the low wetlands along the Kujukuri coastline. Ground surfaces in such bases are often covered with water because of soil compaction, leaving them susceptible to stagnant water. Water stagnation in soil is problematic, potentially interfering with afforesting coastal forests. Therefore, row deep tillage was conducted for parts of growth bases to combat the poor physical properties of the existing soil. Here, we surveyed soil profiles and measured vertical soil hardness distribution in two forest stands with piling up soil to evaluate the effectiveness of tillage for man-made soil. Soil hardness measurements indicated that the vertical areas with ‘soft’ and ‘hard/consolidated’ soil alternately appeared in growth base profiles. Generally, soil of the dense and very hard layers was apt to be formed by strong compaction of the filled-up soil because of heavy machinery usage during growth base preparation. Such dense and hard soils in the untilled areas of the profiles were also observed in this study. By contrast, it was confirmed that row deep tillage drastically improved soil physical properties, i.e., decreased hardness and increased water permeability, because the sequential hardened subsoil layers were well-broken-up. Moreover, it was observed many thick and large roots penetrated deeper layers with deep tillage areas. These results suggest that row deep tillage of hardened soil is quite effective at securing the areas and providing the physical conditions for deeply penetrated roots into deeper soils, which increases healthy root development. They show the effects of soil hardness reduction and water permeability improvement have been maintained for two decades, at least, after construction. These findings will be useful for alleviating some problems of soil compaction, water stagnation, and tree-growth hindrance that have been encountered on afforestation sites with man-made soil.