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HPLC测定利咽解毒颗粒中黄芩苷的含量 总被引:1,自引:0,他引:1
[目的]建立HPLC法测定利咽解毒颗粒中黄芩苷含量的方法。[方法]色谱柱为Agilent zorbax SB-C18柱(4.6 mm×150.0 mm,5μm),柱温30℃,流动相为甲醇-水-磷酸(V∶V∶V=47∶53∶0.2),流速为1.0 ml/min,检测波长为280 nm。[结果]在0.5~100.0μg/ml范围内黄芩苷峰面积与浓度呈良好的线性关系(r=0.999 1),平均回收率为101.14%,RSD值为0.69%(n=9)。[结论]该方法快速、简便、准确,可用于该制剂的质量控制。 相似文献
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In this study, we tested the ability of Baicalin to block Chlamydia trachomatis infection and found that the Baicalin blocked infection of Hep-2 cells. Then, we looked into the expression of RFX5 and CPAF gene in Chlamydia-infected cells. We found that RFX5 and CPAF were up-regulated and down-regulated respectively by Baicalin. Since CPAF is responsible for degrading RFX5, we suggest that CPAF was a primary target of Baicalin and played an important role in regulating RFX5. Our findings demonstrate that Baicalin can inhibit C. trachomatis effectively and therefore, can be considered as potential agents for therapy of Chlamydia infectious diseases. 相似文献
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Shuang–Huang–Lian (SHL), a traditional Chinese formula containing Lonicerae japonicae flos (LJF), Scutellariae radix (SR) and Forsythiae fructus (FF), is commonly used to treat acute upper respiratory tract infection, acute bronchitis and light pneumonia. Forsythoside A is one of the main active ingredients in Forsythiae fructus, a key herb in SHL. In the present study, effects of different compositions in SHL on the in vitro metabolism in Sprague–Dawley rat liver microsomes of forsythoside A were investigated. The observations from Sprague–Dawley rat liver microsomes in the presence of β-NADPH or UDPGA that forsythoside A may be the substrates of CYP3A4, CYP2C9, CYP1A2, UGT1A6, UGT1A3, UGT1A1 and UGT1A9; Chlorogenic acid may be the substrates of CYP3A4, CYP2C9, CYP1A2, CYP2C19, UGT1A6, UGT1A3 and UGT1A1; Baicalin may be the substrates of CYP3A4, CYP2C19, CYP1A2, UGT1A9, UGT1A1 and UGT1A3; Baicalein may be the substrates of CYP3A4, CYP2E1 and UGT1A6. It was also found that the residue of forsythoside A in SHL, FF + LJF and FF + SR was greatly increased compared with that in FF in Sprague–Dawley rat liver microsomes in the presence of β-NADPH or UDPGA, which indicated that the metabolism of forsythoside A in SHL may be influenced by chlorogenic acid in LJF acting on the CYP3A4, CYP2C9, CYP1A2, UGT1A6, UGT1A3 and UGT1A1; baicalin in SR acting on the CYP3A4, CYP1A2, UGT1A9, UGT1A1 and UGT1A3; baicalein acting on the CYP3A4 and UGT1A6 respectively. 相似文献
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HUI Shuang 《园艺学报》2015,31(12):2126-2129
AIM: To investigate the effect of baicalin on the radiosensitization of HeLa cells. METHODS: The cell activity was determined by MTT assay. The radiosensitivity of HeLa cells was detected by colony formation assay. The cell cycle was analyzed by flow cytometry. The protein levels of Akt, p-Akt, Bad and p-Bad were examined by Western blot. RESULTS: The cell growth of the HeLa cells was inhibited by baicalin dose-dependently and IC50 was 43.65 mg/L. The results of colony formation assay showed that combination of 8 mg/L baicalin and radiotherapy further improved survival curve and decreased the value of D0 and Dq, as compared with radiotherapy alone (P<0.05). Furthermore, baicalin enhanced the effect of radiotherapy on cell cycle, as evidenced by the increase in cell percentage in G2/M phase (P<0.05). Additionally, after incubation with baicalin, radiotherapy-induced phosphorylation of Akt and Bad were further augmented (P<0.05). CONCLUSION: Baicalin augments radiosensitivity of HeLa cells through the inhibition of cell cycle transition and activation of PI3K/Akt signaling pathway. 相似文献
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鸡血浆中黄芩苷及绿原酸的HPLC法建立 总被引:1,自引:1,他引:0
建立了测定鸡血浆中复方慢呼抗口服液主要成分黄芩苷和绿原酸的高效液相色谱法.流动相为乙腈、1%冰醋酸,梯度洗脱30 min,黄芩苷和绿原酸的波长分别为280、327 nm,流速1 mL/min.分别采用高氯酸法、甲醇乙腈法、正丁醇法处理血浆,选取最优方法进行考察.甲醇乙腈法处理血浆,方法专属性好,回收率较高,线性关系良好,精密度及准确度较高,可用于药代动力学研究. 相似文献
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为探讨不同浓度黄芩苷对热应激条件下猪近端肾小管细胞(pig kidney proximal tubular cells,LLC-PK1)细胞凋亡率及B细胞淋巴瘤/白血病-2基因(B-cellymphoma-2,Bcl-2)和Bcl-2相关X蛋白基因(Bcl-2 associated X protein,Bax)表达的影响,将培养的LLC-PK1细胞随机分为7个组,Ⅰ组为37℃空白对照组,Ⅱ组为42℃单纯热应激1 h组,Ⅲ、Ⅳ、Ⅴ、Ⅵ和Ⅶ组分别为用不同浓度黄芩苷(0.01、0.1、1、10和100μg/m L)处理组后42℃热应激1 h组,运用实时荧光定量PCR和Western blot分别检测Bcl-2和Bax基因及蛋白的表达,流式细胞仪(Annexin V-FITC/PI双染法)检测细胞凋亡率。结果表明,与Ⅰ组相比,Ⅱ组能显著诱导LLC-PK1细胞Bcl-2 m RNA的表达(P0.05),极显著诱导细胞Bax m RNA和蛋白的表达(P0.01),能极显著降低细胞Bcl-2和Bax m RNA和蛋白的比值(P0.01),极显著增加细胞凋亡率(P0.01),但对细胞Bcl-2蛋白的表达无显著影响(P0.05)。黄芩苷处理组与Ⅱ组相比,Ⅳ、Ⅴ和Ⅵ组LLC-PK1细胞Bcl-2 m RNA的表达量均升高,其中Ⅴ组差异极显著(P0.01),Ⅳ及Ⅵ组差异显著(P0.05),Ⅲ及Ⅶ组差异不显著(P0.05),而细胞Bcl-2蛋白的表达量与Ⅱ组相比均差异不显著(P0.05);同样与Ⅱ组相比,黄芩苷处理的各组细胞Bax m RNA及蛋白的表达量均降低,其中Ⅴ及Ⅵ组差异极显著(P0.01),其余各组差异显著(P0.05);除Ⅲ组外,其他各组细胞Bcl-2和Bax m RNA及蛋白的比值与Ⅱ组相比均显著升高(P0.05),其中Ⅴ组细胞Bcl-2和Bax蛋白的比值差异极显著(P0.01);细胞凋亡率仅有Ⅲ组与Ⅱ组相比差异不显著(P0.05),而Ⅴ及Ⅵ组细胞凋亡率极显著低于Ⅱ组(P0.01),其余的Ⅳ和Ⅶ组显著低于Ⅱ组(P0.05)。一定浓度范围内的黄芩苷(0.1~100μg/m L)可能通过下调热应激条件下LLC-PK1细胞Bax的表达,从而提高Bcl-2和Bax的比值,降低细胞的凋亡率,对细胞起到保护作用。本研究从分子水平研究黄芩苷缓解热应激对猪LLC-PK1细胞的损害作用,可为明确其解热机制提供理论基础,并为其在临床上的应用提供有价值的参考资料。 相似文献
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WU Jun XIA Yuan-yu CHEN Jie XIAO Ling WANG You ZHAO Yuan-yuan YE Gang YIN Qing-qiao 《园艺学报》2018,34(9):1571-1577
AIM:To investigate the therapeutic mechanism of baicalin for diabetic nephropathy involving microRNA-141 (miR-141)/silent information regulator 1 (Sirt1) signaling pathway. METHODS:Mouse glomerular mesan-gial cell line SV40-MES-13 was treated with high glucose (HG, 25 mmol/L glucose) to establish diabetic nephropathy cell model. Baicalin at 100 μmol/L was used to treat glomerular mesangial cells. qPCR and Western blot were performed to determine the expression levels of miR-141 and Sirt1. The regulatory relationship between miR-141 and Sirt1 was detected by dual-luciferase assay. The apoptosis of glomerular mesangial cells was analyzed by flow cytometry. RESULTS:Compared with control group, the cells treated with HG showed increased levels of miR-141 and apoptosis, and Sirt1 expression was decreased (P<0.01). Baicalin and miR-141 inhibitor suppressed the HG-induced effect on the levels of miR-141, Sirt1 and apoptosis. Knockdown of Sirt1 expression reversed the effect of miR-141 inhibitor on the levels of miR-141, Sirt1 and apoptosis. Over-expression of miR-141 reversed the effect of baicalin on the glomerular mesangial cells treated with HG. Up-regulation of Sirt1 abolished the effect of miR-141 over-expression on the glomerular mesangial cells. CONCLUSION:Baicalin inhibits the apoptosis of mouse glomerular mesangial cells via miR-141/Sirt1 signaling pathway, thus attenuating diabetic nephropathy. 相似文献
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