全文获取类型
收费全文 | 4746篇 |
免费 | 229篇 |
国内免费 | 410篇 |
专业分类
林业 | 225篇 |
农学 | 348篇 |
基础科学 | 117篇 |
657篇 | |
综合类 | 1781篇 |
农作物 | 293篇 |
水产渔业 | 184篇 |
畜牧兽医 | 1090篇 |
园艺 | 403篇 |
植物保护 | 287篇 |
出版年
2024年 | 10篇 |
2023年 | 81篇 |
2022年 | 133篇 |
2021年 | 181篇 |
2020年 | 160篇 |
2019年 | 168篇 |
2018年 | 138篇 |
2017年 | 217篇 |
2016年 | 201篇 |
2015年 | 197篇 |
2014年 | 248篇 |
2013年 | 323篇 |
2012年 | 329篇 |
2011年 | 368篇 |
2010年 | 302篇 |
2009年 | 301篇 |
2008年 | 287篇 |
2007年 | 304篇 |
2006年 | 268篇 |
2005年 | 186篇 |
2004年 | 167篇 |
2003年 | 104篇 |
2002年 | 101篇 |
2001年 | 79篇 |
2000年 | 85篇 |
1999年 | 83篇 |
1998年 | 47篇 |
1997年 | 46篇 |
1996年 | 41篇 |
1995年 | 34篇 |
1994年 | 21篇 |
1993年 | 30篇 |
1992年 | 36篇 |
1991年 | 22篇 |
1990年 | 15篇 |
1989年 | 18篇 |
1988年 | 13篇 |
1987年 | 11篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1977年 | 1篇 |
1974年 | 1篇 |
1956年 | 4篇 |
1955年 | 2篇 |
排序方式: 共有5385条查询结果,搜索用时 46 毫秒
11.
12.
利用4-二乙氧基膦酰基-2-酮丁酸乙酯与 L-赤式-(+)-1,乙-二苯基-2-氨基乙醇的缩合,将产物还原、氢解及水解得光活性产物,ee 值67%。 相似文献
13.
本文在文献[1]基础上,运用金相,电化学方法研究了50Mn18Cr4电机护环钢依照QHJ-79试验标准在硝酸盐介质中应力腐蚀开裂(即SCC)的性质,结果指出,此体系中SCC的控制过程为阳极溶解。 相似文献
14.
Selective muscarinic receptor antagonists were used to identify muscarinic receptor subtypes in equine trachealis strips. The M1 receptor antagonist pirenzepine (10–7 mol/L to 3 × 10–5 mol/L) and the M3 receptor antagonist 4-diphenylacetoxy-N-methylpiperidine (4-DAMP, 10–9 mol/L to 3 × 10–7 mol/L3) dose dependently inhibited the contractile responses to electrical field stimulation (EFS) and exogenous acetylcholine (ACh). Schild plots yielded a pA2 value for pirenzepine vs ACh of 6.75 ± 0.09, which is consistent with the affinity for M2 or M3 receptors, and a pA2 value for 4-DAMP vs ACh of 8.47 ± 0.09, which is in agreement with the affinity for M3 receptors. The M2 receptor antagonist gallamine (10–5 mol/L and 10–4 mol/L) did not affect the response of trachealis to exogenous ACh and low-frequency EFS (0.1–2 Hz) but decreased the responses to high-frequency EFS (4–16 Hz). These results suggest that the muscarinic receptors mediating contractions induced by ACh in equine tracheal smooth muscle are of the M3 subtype. The lack of an increase in the response to EFS following gallamine suggests that functional prejunctional inhibitory M2 receptors are not present on the cholinergic nerves innervating equine tracheal smooth muscle. 相似文献
15.
维生素K3的合成及应用 总被引:5,自引:0,他引:5
本文概述了维生素K3的各种合成技术路线,对维生素K3生产技术的发展进行了展望,介绍了其应用和市场供求现状。 相似文献
16.
试验通过不同浓度的Na2B4O7在改变PDA培养基及棉子壳一木屑培养基的试验,测定了其对毛木耳菌丝生长的影响。结果表明:在200mg/kg浓度下促进作用最为显著,而其浓度超过400 mg/kg时对菌丝有强烈的抑制作用。 相似文献
17.
杨衡 《农业图书情报学刊》2005,17(3):154-157
本文从语言特点、编排体例及内容特点出发,对《中图法》(第四版)类目注释进行了系统的认识,并就此提出了自己的一些看法。 相似文献
18.
The effects of root-applied chalcone at 0.15 mmol L−1 on the growth and lignin biosynthesis in maize were investigated. The contents of 4-coumarate: CoA ligase (4CL, EC 6.2.1.12) substrates in maize shoots were increased more rapidly in the samples with chalcone application than in the control and the increase occurred at ≤ 3 h after the application (HAA). The lignin content was reduced by chalcone at ≤ 6 HAA. The shoot growth was suppressed by chalcone at ≤ 9 HAA. Consequently, the results suggest that chalcone suppressed maize growth by inhibiting monolignol biosynthesis. 相似文献
19.
AIM:To manufacture recombinant protein of the highly conserved domain in human bone morphogenetic protein-1(BMP-1) using gene engineering methods as antigen for making wide spectrum antibody to BMP-1.METHODS:We analyzed the gene sequences and protein structures of BMP-1 and its related proteins, and chose a highly conserved fragment as target gene. Total RNA was prepared from human osteosarcoma cell line Saos-2, then the target gene was amplified with RT-PCR. The PCR product was cloned into prokaryotic expression vector pMAL c2 to get recombinant vector BMP-1(322-588aa)-pMAL c2. After transforming the recombinant plasmid into DH5-alpha and screening, several prositive clones were got for sequencing. Finally the transformed cells was induced with IPTG to get fusion protein.RESULTS:The BMP-1 gene fragment was successfully cloned into vector pMAL c2, and was able to express efficiently with IPTG inducement. The amount of expressed fusion protein is about 66%-72% in total volume of bacterial proteins.CONCLUSIONS:The recombinant protein contains several key domains(2 CUB domains and 1 EGF domain), which are shared by BMP-1 and its related proteins. Specific wide spectrum antibody to human BMP-1 and its related proteins may be generated with this recombinant protein antigen. 相似文献
20.
AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P<0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps. 相似文献