Effects of Momordica grosvenori Saponins on Type 2 Diabetes Rats Induced by High-sucrose/High-fat Diet and Streptozotocin

The purpose of the experiment was to study the effect of Momordica grosvenori saponins on improving the effect of high-sucrose/high-fat diet combined with streptozotocin induced type 2 diabetes in rats.The type 2 diabetes rat model was established with a high-sucrose and high-fat diet combined with streptozotocin.Successfully modeled rats were given the total saponins of Momordica grosvenori (100,200,400 mg/kg) or metformin (268 mg/kg) by gavage,once a day for 4 weeks.Recording the "three more and one less"index (24 h internal urine output,water intake and feed intake,body weight);Detecting of biochemical indicators,analysis of fasting blood glucose (FBG),serum total cholesterol (TC),triglycerides (TG),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C),insulin (INS),catalase (CAT),superoxide dismutase (SOD),malondialdehyde (MDA),alanine aminotransferase (AST),aspartate aminotransferase (ALT),blood urea nitrogen (BUN) and creatinine (Cr) levels.Hematoxylin-eosin staining (HE) was used to observe the pathological changes of the pancreas.Compared with the model group,the feed intake,water intake,urine output,blood indicators FBG,INS,MDA,TC,TG,BUN and Cr levels,AST and ALT activities were significantly or extremely significantly decreased in the total saponins of Momordica grosvenori each dose group and metformin group (P<0.05;P<0.01),while CAT,SOD activities and HDL-C level were significantly or extremely significantly increased (P<0.05;P<0.01).In pancreatic tissue,the number of pancreatic cells was increased significantly,and the pathological damage of the pancreas was significantly reduced. Momordica grosvenori saponins and metformin had obvious effects on improving glucose metabolism disorders,and at the same time could significantly reduce insulin resistance,enhance the body’s ability to resist oxidative stress and improve liver and kidney functions.     

猪链球菌2型江苏分离株溶血素基因的检测及序列分析

根据猪链球菌2型(strep tococcus su is type 2)溶血素基因(sly)设计和合成了一对可扩增其完整阅读框的引物,对HA 9801等6株猪链球菌2型江苏分离株、1株德国分离株SS2-D及猪链球菌C群参考株ATCC 35246的核酸进行PCR扩增,结果显示HA 9801等6株江苏分离株及德国株SS2呈阳性,ATCC 35246呈阴性。HA 9801株PCR产物纯化后测序,序列分析结果表明该DNA片段与猪链球菌2型1933株的sly基因同源性为99%。     

NO、TNF和IL-2与新型鸭肝炎病毒感染雏鸭肝脑组织损伤的关系

用新型鸭肝炎病毒人工感染9日龄健康樱桃谷雏鸭，对感染后12、24、48、96、168h和14d雏鸭血液、肝和脑组织中一氧化氮（NO）含量，血液中肿瘤坏死因子（TNF）和白细胞介素2（IL-2）含量进行了测定，同时对感染雏鸭的组织病理学变化进行了观察。结果表明．血清中NO含量在接种后48h开始升高，一直持续到接种后96h，接种后7d恢复正常；肝脏组织中NO含量仅在接种后24h与对照组比较显著升高，在其他时间未表现有差异；脑组织中NO含量在整个试验期间没有变化。血清中的TNF和IL-2含量在接种后24h均表现升高，接种后96h降低，其他时间无改变。感染雏鸭的肝组织在接种后24h表现出血性坏死性肝炎变化，接种后48～96h呈增生性病变，而接种后各时期脑组织均呈非化脓性脑炎变化。由此表明，新型鸭肝炎病毒感染可导致雏鸭体内NO、TNF和IL-2发生变化，并且与肝组织损伤、疾病的发生发展有关。     

泡椒专用品种--辛红1号的选育

辛红1号是由我所收集的国外辣椒品种“胡椒味辣椒”,采用系谱法,经过多代分离、定向选择获得的一个泡椒专用品种。该品种为半辛辣型,尾味带甜,果实硬度好,特适宜泡制。果实圆柱形,青椒深绿色,老熟时大红色,果面有少许网纹,一般667m2产红椒1400kg。经多点试验和生产示范,适宜在我国西南、长江流域和南方大部分地区种植。     

Single nucleotide polymorphism identification, linkage and radiation hybrid mapping of the porcine pituitary adenylate cyclase-activating polypeptide type I receptor gene to chromosome 18

Pituitary adenylate cyclase‐activating polypeptide (PACAP) is a neuropeptide with diverse biological actions. Type I PACAP receptors (PACAPR) are specific for PACAP, whereas type II and III PACAPRs are less restricted. To localize and analyse the variation of this gene, a 559‐bp long intronic fragment of the porcine PACAPR gene was amplified by polymerase chain reaction and sequenced in samples from five different pig breeds. One single nucleotide polymorphism was identified and its allele frequency was determined in all five breeds. Linkage analysis in a Berkshire × Yorkshire reference family placed the PACAPR gene on chromosome 18, between SW787 and S0062 (SW787– 8.1 cM –PACAPR– 3.0 cM –S0062). Radiation hybrid mapping confirmed that the PACAPR gene was linked to SW1682 on chromosome 18 (28.8 cR₃₀₀₀; LOD = 10.4).     

苹果新品种‘天汪1号’

 ‘天汪1号’是从‘红星’的芽变中选育出的苹果短枝型新品种, 在天水地区9月中旬成熟,果实生育期148 d。果实圆锥形, 五棱突起, 果形指数0.90～0.98, 平均单果质量210 g, 果实全面浓红,色相片红; 果肉细而致密, 汁多, 味香甜, 可溶性固形物含量11.9%～14.1%。树势强健, 丰产, 稳产。     

青麻叶大白菜新品种秋玉78的选育

秋玉78是由两个青麻叶类型自交不亲和系F115-93和L132配制而成的中熟大白菜一代杂种。生育期75d(天)左右,叶球高桩直筒形,株高58cm,结球紧实,球高52cm,单球质量3.5~4.0kg;株型紧凑直立,叶片浓绿油亮,核桃纹适中,中肋绿色;高抗病毒病、软腐病,抗霜霉病,耐贮性好,商品性好,粗纤维含量少;每667m2净菜产量7300~7800kg,已在天津、河北、内蒙古、山东、山西、云南等地推广种植520hm2。     

安徽省烟草黑胫病菌的交配型及其地理分布研究

 Total 69 isolates of Phytophthora nicotianae var. nicotianae were isolated and identified from tobacco black shank samples collected from different areas of Anhui province, and mating types of the oomycete were investigated by the method of partnership culture in vitro on L-tryptophan medium with β-sitosterol. The results showed that A2 mating type was the predominant in isolates of P. nicotianae var. nicotianae, next was A₀ mating type, A₁ and other mating types were not observed. It was suggested that sexual reproduction of P. nicotianae var. nicotianae might not take place frequently, and Anhui province might not be the original center of P. nicotianae var. nicotianae. The results indicated further that mating type geographical distribution of P. nicotianae var. nicotianae in Anhui province was in line with that in China. In addition, the significance of the test results and the possible reason for A₁ mating type being absent were discussed.     

广西玉米纹枯病菌培养性状及其致病力研究

从广西玉米主产区的8个县市秋玉米的纹枯病病株中分离出8个玉米纹枯病菌株,经生物性状的观察和菌丝融合群测定表明,它们都属于立枯丝核菌AGk-1-IA融合群;腐生定殖能力和致病力测定表明,菌株GX4、GX1的腐生定殖能力和致病力最强。