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21.
以微生物拮抗病原菌是防治病害安全高效的可行手段。通过对果实表面微生物进行筛选鉴定,以期得到一株可以有效抑制灰霉病的生物防治菌株。对拮抗菌株H-1进行形态学特征、生理生化特性分析,结合16S rDNA序列分析和gyrB 基因序列分析构建系统发育树;采用平板对峙法结合果实伤口试验考察拮抗菌H-1在体外及梨果表面的抑菌作用。结果表明:经鉴定H-I菌株为贝莱斯芽孢杆菌(Bacillus velezensis);B. velezensis H-1在体外条件下可显著抑制灰葡萄孢霉(Botrytis cinerea)、细极链格孢(Alternaria tenuissima)和串珠镰孢菌(Fusarium verticillioides)的菌丝生长,其中对灰葡萄孢霉菌丝的抑制作用最强;果实伤口试验表明,1×1011 CFU/mL的B. velezensis H-1可完全抑制梨果表面灰霉菌生长。由此可见,B. velezensis H-1可以有效抑制梨果采后灰霉病的发生。 相似文献
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Kazuhiko Narisawa Hitoshi Kawamata Randolph S. Currah Teruyoshi Hashiba 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(2):103-109
One hundred and twenty-three fungal isolates were obtained from 225 root segments of eggplants, melon, tomato, strawberry and Chinese cabbage, grown as bait plants in a mixed soil made up of samples from different fields in Shizuoka, Japan. Isolates belonging to Mycelium radicis atrovirens (MRA), including Phialocephala fortinii, were the most prevalent in all the five bait plants. Eleven of the 123 isolates, after being inoculated onto axenically reared eggplant seedlings, almost completely suppressed the pathogenic effects of a post-inoculated, virulent strain of Verticillium dahliae. Seven of these 11 isolates had come from the roots of eggplant and included Heteroconium chaetospira,
P. fortinii, and unidentified species of Fusarium, Penicillium, Trichoderma and MRA. P. fortinii, H. chaetospira, a non-sporulating isolate with white mycelium (SWM) and MRA were easily reisolated from root segments. Hyphae of H. chaetospira, P. fortinii and SWM colonized the root tissues of eggplant without causing apparent pathogenic symptoms. The mechanisms by which these endophytes confer resistance to infection by V. dahliae are unknown but the effectiveness of these fungi in a laboratory setting indicates that they have potential as biocontrol agents and merit further investigation. 相似文献
24.
荔枝、龙眼园使用机油乳剂的安全性及其对荔枝蒂蛀虫的防治研究 总被引:8,自引:0,他引:8
利用机油乳剂对果树进行安全性和防治荔枝蒂蛀虫Conopomorpha sinensis Bradley研究试验,结果表明,喷施机油乳剂后荔枝和龙眼的落果率与对照树4无差异,喷清水的对照树其蒂蛀虫蛀果率比喷机油乳剂的处理树高,分别为150、200和250倍机油乳剂处理的2.8、3.0和2.7倍。在荔枝、龙眼园应用机油乳剂防治害虫不会导致落叶、落果、畸形叶和畸形果,也不会延缓果实的生长和成熟及降低单果重,而有利于控制介壳虫、螨类、蛀果类等害虫。机油乳剂可替代广谱性化学农药的使用,减少化学农药的污染,有利于果园天敌的生存、繁殖,提高生物多样性,改善果园生态环境。 相似文献
25.
甜菜夜蛾性信息素组分的鉴定及其田间试验 总被引:8,自引:0,他引:8
采用气相色谱仪(GC)及气质联用仪(GC-MS)等技术对我国甜菜夜蛾性信息素组分的鉴定结果表明,雌蛾性信息素腺体中含有4种组分,分别为Z9,E12-14:Ac(A)、Z9-14:OH(B)、Z9-14:Ac(C)和Z9,E12-14:OH(D);田间和室内种群各组分的比例(A:B:C:D)分别为47:18:18:17和43:18:23:16,比例及滴度在两种群间未有显著差异;雄蛾田间引诱测定表明,组分A、B显示性信息素活性。几种不同配比的硅橡胶塞诱芯在田间均显示极高的诱蛾活性,以9:1的AB二元诱芯(剂量100μg)最高,其诱蛾量与黑光灯相当,两者呈显著的正相关性,表明该诱芯可替代黑光灯用于甜菜夜蛾的种群测报。利用性诱捕器进行田间种群监测显示,1999年浙江省慈溪市的甜菜夜蛾共发生6代,以第4、5代发生量最高。 相似文献
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Pest Management in Traditional Tropical Agroecosystems: Lessons for Pest Prevention Research and Extension 总被引:1,自引:0,他引:1
Helda Morales 《Integrated Pest Management Reviews》2002,7(3):145-163
Based on current agroecological theory and IPM practices, this review explores the role of traditional practices, involving
site selection, soil management, timing of planting and harvesting, crop resistance, intercropping, weed management, harvest
residue management, post-harvest management, natural enemies management, mechanical control, repellents and traps in the natural
regulation of potential pests. In synthesis, the literature suggests that although pest management professionals focus their
efforts on pest control, the preventative approach taken by traditional farmers is more effective. Potential constraints to
the implementation of this preventive pest management approach include:(1) lack of integration of ecological theory and pest
management, (2) lack of cooperation among social and biological scientists, and (3) lack of real efforts to work with farmers
as equals and support mechanisms that protect their knowledge.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
28.
Denis J. Sonwa Ousmane Coulibaly A. Akinwumi Adesina Stephan F. Weise Mathurin Tchatat 《Integrated Pest Management Reviews》2002,7(3):191-199
Imports of pesticides have long been subsidised for use in the cocoa agroforests of the humid forest zone of southern Cameroon.
With the liberalisation of the cocoa and pesticides sectors and the devaluation of the local currency (CFA franc), farmers
are facing fluctuations in the price paid for cocoa and the high cost of farm inputs. Without the support of the extension
services, they themselves have developed traditional integrated control methods based on the use of plant extracts mixed with
conventional pesticides. From a survey of 300 cocoa farmers, the study assesses the farmers' command of these methods, the
pests controlled, the problems encountered and the institutional constraints in the definition and dissemination of integrated
control methods. The study concludes with recommendations for research and development towards the better definition and dissemination
of integrated control methods.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
29.
O. Coulibaly D. Mbila D.J. Sonwa Akin Adesina J. Bakala 《Integrated Pest Management Reviews》2002,7(3):165-172
Cocoa is a key or source of income and poverty reduction in the humid forest of Southern Cameroon. Cameroon like other African countries went through a major economic crisis in the early 1980s with a decline in international commodity prices and significant changes in macroeconomic policies. Structural adjustment reforms following the economic crisis led to removal of fertilizers and pesticides subsidies, cocoa price liberalization and the overall withdraw of Government interventions from the cocoa sub-sector. Cocoa input price increases have been compounded by the devaluation of the CFA Franc, which doubled the prices of the imported pesticides which were considered key to the control of cocoa pests. This overall economic shock led to changes in cocoa producer's production decisions as a response to minimize cost. Among the changes the use of alternatives to imported chemicals for cocoa pest control. Farmers responded to the high prices of pesticides by developing, from local botanical knowledge and pest management strategies, which include plant extracts and plant extracts mixed with pesticides at different proportions. This is a major decision given the importance of imported chemical in the cost of production of cocoa. Valuable indigenous knowledge from farmers could be used also as an effective support system for communicating and diffusing modern knowledge and technologies to farmers. The paper describes the farmer knowledge-based alternatives to chemical pesticides for pest control in cocoa fields as a response to high pest control costs. Pest management specialists are urged to take advantage of this shift in practice and assess their effectiveness for further use. Two sets of questions are posed: (1) were the conventional insecticides, with all their problems, really necessary? and (2) are the materials derived from locally grown plants effective pest management agents or are they, in some way, placebos? 相似文献
30.