贵州喀斯特地区牧草引种试验评价

对国内外引进的6个禾本科牧草品种,9个豆科牧草品种以及喀斯特地区的4个对照品种,在贵州独山地区进行引种试验。结果表明：各参试品种生长良好,能安全越冬、越夏,一年可刈割5～6次,鲜草产量达36558．2～66450．0kg／hm2,干草产量10771．0～19869．0kg／hm2。综合性状表现较好的依次为鸭茅Totter,Wana,Tuoa;多年生黑麦草Nui,Bronsys;白三叶Alice,C6151,Prop,Pitua,Sustain;百脉根Mareb,Lel。     

对退耕还林还草有关问题的进一步思考

阐述了“一退双还”政策措施提出的意义和实施情况,提出应进一步明确“一退双还”的目的,从思想和政策上解决重林木轻草灌的倾向,力戒形式主义和表面文章、讲求实绩实效,“以林为主”的绿化方针值得进一步深思和商榷,“一退双还”的责任制和利益机制需进一步完善等一系列问题,值得进一步深入思考和认真解决,以保证“一退双还”和国土绿化工作的持续和健康发展。     

高寒地区多年生禾草混播草地种间竞争效应分析

从高寒地区3组分禾草混播草地群落的种问竞争机制出发,建立了适合高寒地区3组分禾草混播草地群落种间竞争机制的Lotka—Volterra竞争效应模型,并对该模型所描述的竞争进行了计算机模拟试验。模拟结果表明,试验所选的3种高寒地区3组分禾草混播草群的种间相容性分为两种类型,即不稳定的群落：多叶老芒麦 无芒雀麦 垂穗披碱草和垂穗披碱草 多叶老芒麦 扁穗冰草,其竞争结局是：群落内多叶老芒麦和垂穗披碱草二者共同表现为优势种群,无芒雀麦或扁穗冰草与优势植物的竞争结果将导致其从群落中消失,多叶老芒麦和垂穗披碱草种群将能稳定共存;不稳定-稳定过渡群落：无芒雀麦 多叶老芒麦 扁穗冰草,在没有多叶老芒麦种群的时候,无芒雀麦和扁穗冰草种群可以共存,但是如果多叶老芒麦种群加入无芒雀麦和扁穗冰草所占的区域,它将导致无芒雀麦和扁穗冰草种群趋于灭绝。通过对3个混播试验组计算机模拟竞争模型的竞争效应分析,其结果与方法对科学建植同类型的多年生混播人工草地具有一定的指导意义。     

不同秋眠类型苜蓿品种生产性能与持久性分析

通过对58份不同秋眠类型苜蓿品种再生性、持久性和产草量在呼和浩特的测定分析,结果表明:1)在全部参试材料与秋眠类型组中,苜蓿秋眠级和再生性正相关并达到极显著、显著水平;而在半秋眠类型组、非秋眠类型组中,苜蓿的再生性和秋眠级相关性很小。2)在全部参试材料、半秋眠类型组、非秋眠类型组中,首蓿的持久性与秋眠级负相关,但是在秋眠类型组中,首蓿秋眠级和持久性相关性很小。3)在全部参试材料、半秋眠类型组、非秋眠类型组中,苜蓿的产草量和秋眠级负相关;在秋眠类型组中,苜蓿的产草量和秋眠级正相关但不显著。     

陕西省耕地面积变化趋势及其驱动因子研究

以实地调查和统计资料,分析了陕西省1949-2003年55 a间耕地面积变化的总体趋势.运用主成分分析方法定量分析了耕地面积变化的驱动因子,结果表明:社会-经济-科技综合作用、退耕还林等2大类因素是影响耕地面积变化的2个主成分.通过建立多元回归模型,对陕西省2010年和2015年的耕地面积进行了预测.研究结果将为合理利用和保护耕地,为干旱地区农业可持续发展提供科学依据.     

色素万寿菊品比试验与相关分析

在露地栽培了9个品种的色素万寿菊,调查其株高、茎粗、株幅、花径、产量等指标,并对这些性状进行相关分析,结果表明:Ⅲ号品种的产量最高,各项形态指标优良,适合露地种植;Ⅰ和Ⅵ号产量虽次于Ⅲ号品种,其它形态指标与Ⅲ号品种相差不多;Ⅸ和Ⅷ号品种产量最低,其余品种表现介于Ⅵ和Ⅸ号之间;小区产量与株高、花径、平均花瓣层数呈极显著正相关,与株幅、单株花数呈显著相关.     

应用基因芯片检测动物性食品中主要致病菌

依据16SrDNA、23SrDNA上的恒定区和可变区设计7种致病菌的通用引物和特异性寡核苷酸探针，并对探针5’端进行氨基化修饰进行基因芯片共价结合，然后优化杂交反应液、芯片点样及后处理程序，研制出一种可同时检测动物性食品中7种主要致病菌的基因芯片。结果表明，7种致病菌的基因芯片杂交检测灵敏度可达10^2cfu／g，与经典传统检测方法及分子生物学、免疫学方法相比该方法特异性强，准确率高，可同时检测动物性食品中7种致病菌。     

Molecular characterization of Miraflores peach variety and relatives using SSRs

Some traditional peach varieties, originated from the region of Aragón (Spain), were analysed by SSRs (simple sequence repeats). The aim of this research was to characterize 19 clones related to Miraflores variety, with unknown pedigrees, to assess their genetic diversity and to elucidate their possible relationships with 10 traditional peach varieties. Twenty SSR primer pairs with high levels of polymorphism, which have been previously developed for peach, were used in this study. A total of 46 alleles were obtained for all the microsatellites studied, ranging from one to six alleles per locus, with a mean value of 2.3 alleles per locus. Fourteen SSRs were polymorphic in the set of varieties studied and permitted to distinguish 16 different genotypes out of the 30 initially studied, although fourteen ‘Miraflores’ clones showed identical gel profiles. The genetic distance matrix was used to construct neighbor joining cluster and to perform principal coordinate analysis which allowed the arrangement of all the genotypes according to their genetic relationships. The genetic relationships among these traditional peach varieties, and in particular among ‘Miraflores’ clones are discussed. The obtained results confirm that microsatellite markers are very useful for these purpose.     

Total antioxidant activities,phenolics, anthocyanins,polyphenoloxidase activities of selected red grape cultivars and their correlations

Sixteen red grape cultivars (Md. Jean Matthias, Öküzgözü, Muscat Hamburg, Cabarnet Sauvignon, Tekirda? Çekirdeksizi, Gewürztraminer, 2B/56, Kalecik karas?, Carignan, Kokulu Siyah, Alfonse Lavallée, Bo?azkere, Adakaras?, Papazkaras?, Mourvedre and Cinsaut) which are grown in Tekirda? Viticulture Research Institute were analyzed for determination of antioxidant activities, total phenolics, anthocyanins, external colour, polyphenoloxidase activities, sugar and acidity. The lowest antioxidant activity (percentage of inhibition on peroxidation in linoleic acid system; AA) was determined in Tekirda? çekirdeksiz extracts as 87.58% and the highest AA was in Mourvedre (93.78%). Total phenolic content (TP) varied from 817 to 3062 μg/ml GAE in the same varieties, respectively. The total anthocyanin (TA) content ranged from 40.3 to 990.8 mg/l fresh weight. The lowest PPO activity was found in Bo?azkere and the highest was in Kalecik karas?. The total sugar content in the analyzed varieties varied between 13.29 and 24.46%. There was stronger correlation between AA and TP than AA and TA.     

农牧交错区耕地净第一性生产力动态变化研究——以陕西榆林市榆阳区为例

以榆林市榆阳区1984～2003年各乡镇的粮食产量统计数据为依据,选用了适合农用地的净第一性生产力估算模型,对该区耕地净第一性生产力单位面积量及总量进行分区动态测算,以揭示生态环境对耕地净第一性生产力的影响.研究表明:(1) 1984～2003年间,榆阳区耕地净第一性生产力总量在波动中上升,20 a内总物质量从21.94×104 t/a增加到57.98×104 t/a,年度递增率为5.24%.(2) 在1984～2001年间,榆阳区北部耕地净第一性生产力远高于南部,且呈较快上升趋势,而南部黄土丘陵沟壑区的耕地净第一性生产力呈较平缓的波动变化,且略有下降.(3) 在1984～2001年间,榆阳区城区、近郊、远郊的差异性在单位耕地面积净第一性生产力上体现为城区＞近郊＞远郊;在耕地NPP总量方面,城区、近郊和远郊都表现为在波动中上升,三区变化以远郊波动幅度最大.(4) 榆阳区耕地净第一性生产力总量的大幅增长有农业投入增加的原因,但主要得益于该区的生态环境的改善.