Clinical efficacy of diclofenac sodium and flunixin meglumine as adjuncts to antibacterial treatment of respiratory disease of calves

Objective To compare the efficacy of the non-steroidal antiinflammatory drugs, diclofenac sodium and flunixin meglumine as adjuncts to the antibiotic treatment of bovine respiratory disease (BRD). Procedure We randomly allocated 80 Holstein calves with BRD to three groups. All the calves received a dose of 2.5 mg/kg tulathromycin by single subcutaneous injection and two of the groups received, in addition, either 2.5 mg/kg diclofenac sodium as a single intramuscular injection (diclofenac group, n = 30) or 2.2 mg/kg flunixin meglumine as an intravenous injection on the first three consecutive days after tulathromycin administration (flunixin group, n = 30). All calves were given a clinical score prior to initial treatment (day 0) and after treatment (days 1, 2, 3, 7 and 14) by observing appetite, demeanour, rectal temperature, the rate and type of respiration, presence or absence of coughing, and nasal discharge. Results During the first 48 h, improvement of adverse signs of respiratory disease, such as pyrexia and elevated respiratory rate, and of a high clinical index score was significant in the two adjunct groups compared with the calves receiving antibiotic alone. The reduction in pyrexia was greatest in the diclofenac group. There were no statically significant differences between treatment groups with regard to eventual perceived recovery from respiratory disease in 14 days. Conclusion In this trial, a single intramuscular dose of diclofenac sodium was equally effective as three intravenous injections of flunixin meglumine given on consecutive days as adjunctive therapy for BRD.     

Multi‐channel visual evoked potential as an ancillary tool to diagnose intraorbital optic nerve lesions

Multi‐channel visual evoked potential (MVEP) recording method was used to assist in diagnosing a 4‐month‐old Chinese Albino rabbit with an intraorbital mass. Subcutaneous MVEP of its both eyes were recorded simultaneously using 16 electrodes (4 × 4) multi‐channel array. Analysis of the cortical potential landscapes (CPL) showed that the conduction function of right eye was remarkably impaired in terms of decreased amplitudes and prolonged latencies. Specific side‐dominant distribution asymmetry of the decreased MVEP amplitudes indicated that the temporal side of the optic nerve (ON) was severely involved. Overall prolonged latencies of the CPL without side differences suggested that the functional impairment could have been caused by the mechanical compression exerted by an intraorbital mass. Surgical removal procedures confirmed that the mass was located temporally to the ON. Pathological examination provided a final diagnosis of a giant polycystic mucocele. Beyond its significance as a standard tool to assess functional changes of the visual pathway, MVEP recordings might assist locating intraorbital lesions that involve the ON by careful analysis of abnormal CPLs.     

野生稻抗病虫基因的挖掘和利用

本文综合国内外文献,简要介绍了野生稻抗病虫基因发掘、定位、克隆及育种应用研究的进展,讨论了野生稻优异抗病虫基因在水稻育种中的应用前景。     

林木病虫害防治施药机的风机选型分析

通过对常用典型风机工作性能和结构特点的分析,结合林木病虫害风送施药防治的要求,提出针对不同高度林木风送施药防治病虫害设备在选用风机类型时的建议和原则。     

兴安盟扎赉特旗杨树病虫害的种类及其防治措施

对本地区杨树烂皮病的抗性树种进行了调查,筛选出最适合本地区栽植的抗病品种——小乘黑（P.Canadensis）和银中杨（P.alba‘Berolinensis’）。对天牛成虫进行了不同药剂不同浓度的防治对比试验,筛选出最合适的药剂——绿色葳蕾250倍液和触破800倍液。对其他地区的杨树病虫害的预防和控制能起到一定的借鉴作用。     

烟叶不同生育期病虫害防治要点分析

充分了解和掌握病虫害发生规律及传染途径,做到防重于治,科学、合理、安全使用农药,才能在病虫害防治方面取得明显效果。     

甘蓝夜蛾核型多角体病毒杀虫剂的研制及药效测定

用麦胚人工饲料饲养甘蓝夜蛾幼虫,饲料添毒接种生产甘蓝夜蛾核型多角体病毒(MbNPV,东北农学院分离的菌株)。以2×10~6PIB/ml接种8龄末期幼虫为最佳接种条件,增殖效果最好,产量最高达4.55×10~9PIB/虫。自制MbNPV可湿性粉剂,室内毒力测定的LC_(50)为4.89×10~4PIB/ml,杀虫效力明显优于液剂。     

植物内生真菌作为生防因子的研究进展

本文对内生真菌作为生防因子的研究进展进行了综述,主要包括内生真菌在植物抗病性、抗虫性、抗逆性等方面的研究情况及其在生产中应用存在的问题.     

PCR技术在农药学研究中的应用

聚合酶链式反应具有灵敏度高、特异性强和操作简便等优点,是应用最为广泛的分子生物技术之一。目前,农药学越来越多的研究涉及分子和基因,PCR技术有着广泛的应用前景。本文简要介绍PCR技术的基本原理和特点,以及其在靶标生物抗药性研究、农药环境毒理、农药作用机理、生物农药的开发等方面的应用。     

东莞莲花山自然保护区昆虫资源的初步研究

本文根据2001和2002年的调查结果，记述了广东省东莞莲花山自然保护区的昆虫种类调查结果．共计11目69科193种。