弹齿滚筒式捡拾装置弹齿的静力学分析

针对弹齿滚筒式捡拾装置在工作过程中弹齿变形现象,对弹齿滚筒式捡拾装置弹齿进行静力学分析,以寻求解决的途径。通过对捡拾装置弹齿在3个工作阶段上的载荷进行分析后,获得各工作阶段弹齿的受力简图,通过比较判断出在捡拾升运阶段弹齿受力比较复杂。以9KJ-1.4A型压捆机捡拾装置为实例,经过计算在有随机载荷和无随机载荷时弹齿齿臂末端的应力情况,得出结论:在没有随机载荷的情况下,齿根处应力远小于其许用应力,弹齿产生弹性变形,有足够的强度;当有随机载荷时,对弹齿产生冲击,若随机载荷过大,垂直于弹齿轴向的分力大于24N,则弹齿会产生塑性变形。对捡拾装置进行静力分析,可为捡拾装置整体结构优化设计供理论依据。     

我国竞技体育竞技实力格局嬗变研究

近几届奥运会，我国竞技体育竞技实力格局发生了变化。主要采用数理统计法和逻辑分析法，从近几届我国代表团的金牌分布演变、奖牌分布演变、优势与潜优势项目演变等方面进行了分析。研究结果表明：1金牌分布点逐渐增加；2奖牌分布面更广；3游泳成为金牌和奖牌的新增长点，已经成为重点优势发展项目；4我国竞技体育整体竞技水平明显提高，优势项目和潜优势项目结构发生了调整。最后，结合我国竞技体育竞技实力格局嬗变现实情况，提出了几点思考。     

高校图书馆竞争情报分析和实施过程初探

介绍了竞争情报在我国的发展,提出了高校图书馆竞争情报的实施过程,以及在此过程中要遵循的原则和建议.     

西瓜抗枯萎病基因同源序列的克隆与分析

本研究根据已克隆的抗枯萎病基因的NBS保守结构域设计了22条上游简并引物和17条下游简并引物,以西瓜抗枯萎病种质PI296341-FR和感枯萎病品种97103为材料,获得了7条来自基因组DNA的RGA序列（GenBank登录号：DQ156558-DQ156564）,均含有NBS保守区的P-环、kinase-2或kinase-3等抗病基因的特征序列结构,所编码的氨基酸序列与已知抗枯萎病基因Fom-2、I2C-1、I2C-2和I2等编码的氨基酸序列表现出11%～72%的同源性,其中来自PI296341-FR的RGA序列175R1与甜瓜抗枯萎病基因Fom-2的同源性最高,为72%。来自PI296341-FR与97103的RGA序列之间同源性较高（73%～97%）,证明了抗病基因在进化上的保守性。     

蜱免疫球蛋白结合蛋白研究进展

蜱体内的免疫球蛋白结合蛋白（immunoglobulin binding protein，IGBP）主要存在于血淋巴和唾液腺中，可以与进入蜱血淋巴中的宿主免疫球蛋白（immunoglobulin，Ig）结合，从而避免宿主免疫球蛋白对蜱内部器官的损害。免疫球蛋白结合蛋白结构和功能的进一步阐明，可为抗蜱疫苗的研发提供新的思路。本文综述了免疫球蛋白结合蛋白的发现及其研究进展。     

应用竞争PCR检测丙酸盐对体外培养牛肝细胞丙酮酸羧化酶mRNA水平的影响

根据牛肝细胞内PC(丙酮酸羧化酶)基因组与PCcDNA相比多含有一个内含子序列．在其中再插入一外源DNA序列．从而使最终构建的突变体片段的长度大于PCcDNA的长度，成功构建了PCcDNA的竞争DNA模板，然后应用竞争PCR方法研究了丙酸盐对体外培养新生牛单层肝细胞PCmRNA水平的影响。使单层肝细胞培养液中丙酸钠浓度分别为0、1．5、2．5、3．5、4．5、8．5、11．5mmol／L，处理24h，提取总RNA、逆转录，在同一体系中用相同引物扩增目的带和竞争模板带。结果表明．随着丙酸钠浓度的升高．PCmRNA水平呈上升趋势，提示肝细胞内PCmRNA的表达水平受培养液中丙酸钠浓度的影响。     

Salmonid fish viruses and cell interactions at early steps of the infective cycle

A flow cytometric virus-binding assay that directly visualizes the binding and entry of infectious pancreatic necrosis virus (IPNV), infectious haematopoietic necrosis virus (IHNV) and virus haemorrhagic septicaemia virus (VHSV) to several cell lines was established. The highest efficiency of binding was shown by the BF-2 cell line and this was used to study, at the attachment level, the interactions of these cells with salmonid fish viruses in coinfections, and to further determine if the earliest stage of the viral growth cycle could explain the previously described loss of infectivity of IHNV when IPNV is present. Our results demonstrated that IPNV binds to around 88% of cells either in single or dual infections, whereas IHNV attachment always decreased in the presence of any of the other viruses. VHSV binding was not affected by IPNV, but coinfection with IHNV reduced the percentage of virus-binding cells, which suggests competition for viral receptors or co-receptors. Internalization of the adsorbed IHNV was not decreased by coinfection with IPNV, so the hypothetical competence could be restricted to the binding step. Treatment of the cells with antiviral agents, such as amantadine or chloroquine, did not affect the binding of IPNV and VHSV, but reduced IHNV binding by more than 30%. Tributylamine affected viral binding of the three viruses to different degrees and inhibited IPNV or IHNV entry in a large percentage of cells treated for 30 min. Tributylamine also inhibited IHNV cytopathic effects in a dose-dependent manner, decreasing the virus yield by 4 log of the 50% endpoint titre, at 10 mm concentration. IPNV was also inhibited, but at a lower level. The results of this study support the hypothesis that IHNV, in contrast to VHSV or IPNV, is less efficient at completing its growth cycle in cells with a simultaneous infection with IPNV. It can be affected at several stages of viral infection and is more sensitive to the action of antiviral compounds.     

Expression of Gab2 in human osteosarcoma U2-OS cells

AIM: To investigate the expression of Grb2-associated binding protein 2 (Gab2) in human osteosarcoma cells and its relationship with the invasion and metastases of human osteosarcoma cells. METHODS: The technique of small RNA interference was used to transfect human osteosarcoma U2-OS cell lines. Western blotting and RT-PCR were used to detect the protein and mRNA expression of Gab2 in transfected U2-OS cells. After transfection, through chemotaxis and invasion assays in vitro, the cell migration and invasion abilities were detected. RESULTS: After transfection, the expression of Gab2 at mRNA and protein levels in Gab2 siRNA transfected cells (SiGab2/U2-OS) was lower than that in scrambled siRNA transfected cells (Scr/U2-OS) and U2-OS cells. After stimulation with epidermal growth factor (EGF) at concentration of 10 μg/L, the migration SiGab2/U2-OS cells was significantly less than Scr/U2-OS cells and U2-OS cells (P<0.01). The number of invasion cells of SiGab2/U2-OS group was significantly lower than the other 2 control groups (P<0.01). CONCLUSION: Inhibition of Gab2 expression obviously attenuates the migration and invasion abilities of human osteosarcoma U2-OS cell line.     

植物MYB蛋白与相应靶DNA结合位点间的相互作用

MYB转录因子家族是功能多样、数量众多的转录因子之一,参与调控植物发育、代谢和对逆境胁迫响应等多种生理过程。近些年研究人员一直致力于研究MYB转录因子的特点和功能分析,目前对转录因子和其靶DNA相互作用已有了解,但对于植物R2R3-MYB蛋白与它们的同源靶DNA相互作用的分子机制的理解并不完整。本文结合最新研究进展,综述了植物MYB转录因子家族的进化、生物学功能以及表达调控,并着重阐述了植物MYB蛋白与相应靶DNA间相互作用特性,为进一步分析功能未知的植物MYB转录因子提供了参考。     

Analysis of Competitive Ability among Clones of the Palisade Grass, Brachiaria brizantha (Hochst. Ex. A. Rich.) Stapf.

Four clones of palisade grass, Brachiaria brizantha (Hochst. Ex. A. Rich.) Stapf., were studied in a diallel competition trial to investigate their competitive relationship in clonal mixtures. Clone X associate interactions were observed for plant height, number of tillers per plant and dry matter yield per plant. Both general and specific competitive abilities were observed for tiller number and dry matter yield. Clone X associate interaction for plant height was mainly due to the specific competitive ability of individual combinations. The most competitive clone observed was CIAT 6387. Competitive abilities of local clones were low compared to the imported clones. Local clones (RU 127 and RU 139) yielded more than imported clones (CIAT 6387 and PI 292185) under low competitive stresses. General and specific competitive abilities should be taken into consideration when mixing clones of palisade grass.