Studies on the antibody response and side effects after intramuscular and intraperitoneal injection of Atlantic lumpfish (Cyclopterus lumpus L.) with different oil‐based vaccines

Atlantic lumpfish (Cyclopterus lumpus L.) is used as a biological delousing agent for sea lice (Lepeophtheirus salmonis K.) infestations in Norwegian aquaculture. Here, we present a study on the antibody response and vaccine side effects after intramuscular and intraperitoneal injection of lumpfish with two vaccines. Both vaccines contained bacterial antigens from atypical Aeromonas salmonicida A‐layer types V and VI, Vibrio anguillarum serotype O1 and Moritella viscosa sp., but one vaccine contained a vegetable oil‐based adjuvant, while the other contained a mineral oil‐based adjuvant. Intramuscular injection of the mineral oil‐based vaccine caused a high acute mortality of fish within 48 hr after immunization. Intraperitoneal injection of the mineral oil‐based vaccine resulted in a lower severity of intra‐abdominal side effects than the vegetable oil‐based vaccine. Intramuscular injection of the mineral oil‐based vaccine resulted in a significantly higher antibody response against A. salmonicida when compared to controls and the vegetable oil‐based vaccine group. The antibody response was poor against V. anguillarum and M. viscosa for all groups. Our results indicate that intramuscular injection of oil‐based vaccines might be feasible for providing immunological protection for Atlantic lumpfish against bacterial diseases, especially atypical A. salmonicida, but more work is required to identity optimal adjuvants.     

Efficacy of a polyvalent injectable vaccine against Flavobacterium psychrophilum administered to rainbow trout (Oncorhynchus mykiss L.)

Flavobacterium psychrophilum is one of the most important pathogens affecting cultured rainbow trout (Oncorhynchus mykiss). Recent information from UK salmonid farms showed country‐wide distribution of genetically and serologically divergent clones, which has hampered the development of a vaccine for rainbow trout fry syndrome. The current study assessed the efficacy of an injectable polyvalent vaccine containing formalin‐inactivated F. psychrophilum in rainbow trout. The vaccine was formulated with an oil adjuvant (Montanide ISA 760VG) or formalin‐killed cells alone. Duplicate groups of trout (60 ± 13 g) were given phosphate‐buffered saline or vaccine formulated with Montanide by intra‐peritoneal (i.p.) injection and challenged by intra‐muscular (i.m.) injection with a homologous and a heterologous isolate of F. psychrophilum at 525 degree days post‐vaccination (dd pv). Significant protection was achieved in vaccinated fish (p = 0.0001, RPS 76% homologous, 88% heterologous). Efficacy of the adjuvanted vaccine was also demonstrated by heterologous challenge at 1155 dd pv resulting in 100% protection, whereas survival in the un‐adjuvanted group was not significantly different from control fish. Levels of specific antibody at 1155 dd pv, as measured by ELISA, were significantly higher in the fish vaccinated with adjuvant when compared with unvaccinated fish.     

中药有效成分对动物疫苗的免疫佐剂效果及相关机理研究进展

佐剂作为疫苗的重要组成部分,其自身的安全性及对疫苗的免疫增强效果受到广泛重视。动物疫苗佐剂种类较多,包括铝佐剂、油乳佐剂和脂质体佐剂等。但由于可应用的佐剂有限,疫苗的需求量增大,因此寻求更加安全有效的疫苗佐剂成为新的研究热潮。中药具有易获取、毒性低和免疫活性强等特点,有潜力成为新型疫苗佐剂。笔者对中药有效成分的佐剂活性、联合疫苗后的免疫调节效果及作用机制进行综述,阐明中药有效成分作为动物疫苗佐剂的可能性及研究进展,为新型佐剂的研制提供理论依据。     

Effects of Methylated Soybean Oil Adjuvant on Fomesafen Efficacy to Weeds

Tank-mix adjuvant has the potential to improve the weed control efficacy of post-emergence herbicides. In order to study the synergistic effect of adjuvant, the effects of different rates of fomesafen alone or applied methylated soybean oil adjuvant（MSO） were sprayed on redroot pigweed, abutilon and black nightshade under greenhouse condition. The results showed that fomesafen had different performance on the three weeds, and MSO adjuvant could effectively increase the control. The nightshade control was lower than other two weeds with all the fomesafen doses from 131.25 to 506.25 ga.i. · hm-2 with or without adjuvant. The control of abutilon was between the black nightshade and the redroot pigweed, and had better control at 375 ga.i. · hm-2 with adjuvant or 506.25 ga.i. · hm-2 alone or with adjuvant respectively. The results indicated that mixing adjuvant with fomesafen improved the control on weeds, especially at the low rate. Black nightshade was more difficult to control. The redroot pigweed had the most susceptibility to fomesafen alone or with adjuvant.     

3种新型助剂与30％多菌灵·戊唑醇悬浮肃混用对苹果枝干轮纹病的防效评价

本文通过田间试验，确定了30％多菌灵·戊唑醇SC与施伴、百渗及百湿露3种新型助剂混用后对苹果枝干轮纹病的田间防治效果。结果表明30％多菌灵·戊唑醇SC 800倍与施伴1500倍、百渗3000倍、百湿露3000倍混用防效分别为78．23％、88．71％、71．77％。不添加助剂的处理30％多菌灵·戊唑醇SC 800倍的防效仅为63．71％。     

风洞环境下喷头及助剂对植保无人飞机喷雾飘移性的影响

为探究和减少植保无人飞机喷雾施药过程中的雾滴飘移,采用由单个旋翼与喷头组成的喷雾单元,在可控风洞环境条件下进行了模拟飞行喷雾试验,控制风洞条件为风速5 m/s、喷雾压力0.3 MPa及旋翼转速2300 r/min不变,对比研究了11种喷头、4种代表性助剂以及不同温度/相对湿度条件对雾滴飘移的影响,采用飘移潜在指数(DIX)及相对减飘率(DPRP)两项指标进行对比评估。结果表明:在温度/相对湿度为20℃/RH 80%条件下,不同类型喷头喷雾药液在空中垂直面和水平距离上的飘移沉积量分布均呈现显著的规律性变化趋势,与对照喷头F110-03相比,喷头飘移潜在性从大到小依次为:TR80-0067>ST110-0067>XR110-01>ST110-015>TR80-01>ST110-02>XR110-03>对照F110-03>IDK系列,其中IDK120-01与IDK120-015喷头的减飘移效果相近并为最好;在30℃/RH 40%条件下,采用XR110-01喷头,分别添加助剂0.5%Silwet DRS-60、1.0%"迈飞"(MF)和1.0%Y-20079后,与不添加助剂的对照相比,平均减飘率分别为43.3%、15.6%和5.2%,表明不同助剂对飘移的影响不同,需考虑助剂类型及其减飘效果合理选用;在20℃/RH 40%、20℃/RH 80%、30℃/RH 40%和30℃/RH 60%条件下,XR110-01喷头与添加1.0%MF助剂组合有利于空中飘移的减少,尤其是高温/低湿条件下,添加助剂的减飘移效果较好。该研究结果可为植保无人飞机的喷头选择、喷雾助剂筛选和实际应用提供参考和指导,并为进一步研究喷头及助剂的减飘技术提供数据基础。     

鸽新城疫(ND)蜂胶佐剂灭活疫苗免疫效果对比试验

采用鸽新城疫ND-GS 01株毒为种毒,分别研制出了尿囊液毒蜂胶佐剂灭活疫苗和四元材料(鸡胚尿囊液、羊水、尿囊膜、羊膜)混合病毒蜂胶佐剂灭活苗。对研制的两种灭活疫苗经安全性检验、急性毒性试验,免疫抗体消长水平与免疫持续期对比试验,结果表明,四元材料的疫苗与尿囊液疫苗性能一致。     

加拿大一枝黄花化学防除配方优化研究

采用L9(34)正交试验,探讨草甘膦与盖灌能混用对加拿大一枝黄花防除效果的影响,结果表明:使用配方为41%草甘膦3 L·hm-2+48%盖灌能2.4 L·hm-2+100%有机硅助剂300 g·hm-2兑水450 L,对加拿大一枝黄花防除效果最好,且成本最低。     

预防犊牛腹泻病W／O佐剂的研究及试验

犊牛腹泻病是由致病性大肠杆菌，沙门氏杆菌，轮状病毒，粘膜病病毒等７种病原引起，由于病原复杂，制造疫苗困难，目前尚无有效的免疫制剂，本试验给出生后５－７天内的犊牛腹膜腔内注射自制的稳定油包水乳剂，称为“佐剂”不加特异性抗原，谓之“替代性接种”，试验结果表明，１０１４头犊牛注射核佐剂后，仅有１１９头发病，保护率为８８％，对照组发病率９５．３％，证明此佐剂可推广应用。