转基因枳橙中rolABC基因荧光定量表达分析方法的建立

以转rolABC基因枳橙B、D、E系及野生植株为材料,制备标准品,采用SYBR Green I染料,构建rol基因和β-actin基因的双标准曲线,校正引物扩增效率后,用2-△△Ct法对rol基因在嫩茎、嫩叶、功能叶、树皮和根中的mRNA水平进行相对定量,建立适合于rolA、rolB、rolC基因实时荧光定量RT-PCR分析方法.初步认为rolC基因在嫩茎、嫩叶、功能叶和树皮中表达量最高;其次是rolA基因,主要在嫩茎中表达;rolB基因表达量最低,主要在根系中表达.     

Vesicular-arbuscular mycorrhizae screened for Troyer citrange

Summary Troyer citrange is an important citrus root-stock but a slow grower. Eighteen different vesicular-arbuscular mycorrhizal (VAM) fungi were screened for their symbiotic response with Troyer citrange in unsterilized soil. Of the 18 fungi,Glomus macrocarpum (Ruakura),G. caledonicum (Nedlands),G. velum (Nedlands),G. monosporum (Nedlands),Gigaspora margarita (Ruakura), gave the greatest improvements in growth and nutrition, resulting in larger leaf area, plant height, stem diameter, and plant biomass, with higher P, Zn and Cu contents. All the five fungi were statistically on a par with each other in improving the growth and nutrition of Troyer citrange. Inoculations with these fungi allowed the rootstock to reach a stem diameter of pencil thickness, i.e., ready for budding, in about 13–14 months in contrast to 18–19 months without mycorrhizal inoculations, thus saving about 5–6 months.