东亚飞蝗FK506结合蛋白基因克隆及其免疫功能分析

为明确东亚飞蝗Locusta migratoria manilensis免疫相关FK506结合蛋白(FK506 binding protein,FKBP)的功能,了解FKBP对金龟子绿僵菌Metarhizium anisopliae侵染东亚飞蝗的影响,分离克隆获得FKBP46基因及其纯化蛋白,采用荧光定量PCR方法测定...     

绿僵菌防治草原蝗虫技术推广中存在的问题及对策

对国内外应用绿僵菌防治草原蝗虫效果进行了分析，结果表明：绿僵菌作为防治草原蝗虫的专性生物制剂，可以持续有效控制草原蝗虫种群密度在经济受害水平以下，对其它草地昆虫无害，无二次中毒，不污染环境，具有推广价值。同时，针对绿僵菌在防治草原蝗虫技术推广中存在的问题，提出了相应的对策。     

4种药剂防治桉树幼林白蚁试验

为防治土栖白蚁危害桉树幼林，选择4种药剂进行桉树容器苗浸根后上山造林试验和白蚁致死驱避试验。结果表明：容器苗经4种药剂浸根后造林，以绿僵菌复合剂的防治效果最好，幼林白蚁危害致死率仅3．4％；施用萘丸的防治效果最差，幼林白蚁危害致死率达20．5％，专项驱避试验对白蚁的驱避作用也不明显。文章还分析了几种灭除驱避白蚁药剂的成本和使用时应注意的问题。     

金龟子绿僵菌的生物学特性及毒力测定

从东北大黑鳃金龟幼虫僵尸体内分离出一株金龟子绿僵菌。系统研究了该菌株的生物学特性,并对其对东北大黑鳃金龟幼虫的毒力进行了测定,获得了LD50、LT50毒力指标及回归方程。研究结果表明在对湿度保持在90%时,无光照条件下该菌株在PPDA培养基中生长良好,最适培养条件为温度25℃、pH值6-8。毒力测定表明,其对东北大黑鳃金龟幼虫具有高毒力,具有很好的应用前景。     

Virulence and mycotoxic effects of Metarhizium anisopliae on Mahogany shoot borer, Hypsipyla robusta (Lepidoptera: Pyralidae)

Developing appropriate control measures for the Mahogany shoot borer,Hypsipyla robusta Moore has become increasingly important due to the severe damaging effect of the pest on the establishment of the saplings of Swietenia mahagoni Jacq(Sapindales:Meliaceae).Existing management methods are largely limited to silvicultural practices and spraying of chemical insecticides.To identify a potential fungal biocontrol agent,we compared the virulence of six native and two standard ARSEF isolates of Metarhizium anisopliae Metsch.against this pest.The average survival time and conidial yield of IWST-Ma7 was higher(6.2 to 7.3 days and 4.9 to 4.7 × 10 5 conidia/ml) than the standards.Significant difference in sporulation on the cadavers between isolates,doses and incubation periods were substantiated for the selection of potential strain.The mycotoxic effects of crude soluble protein extract when incorporated in the artificial diet,the ARSEF 2596 and ARSEF 3603 showed LD 50 value of 3.7% and 5.6%.However,IWST-Ma7 was highly lethal with significant lowest LD 50 value of 2.6%.The enzyme activity of IWST-Ma7 was highest for chitinase,CDA,protease and lipase viz.,1.90 U/mg,1.80 U/mg,0.98 U/mg and 0.80 U/mg respectively.However the enzyme activity of chitinase and Chitin deacetylase assay for all the isolates was significantly higher than protease and lipase activity.The ITS regions(5.8S rDNA and 28S rDNA) of seven isolates of M.anisopliae were amplified using the ITS1 and ITS4 primers which was a unique fragment of approximately 550 bp.Based on ITS regions,phylogenetic tree have been constructed and the isolates have been grouped in to 5 clades.The virulence and mycotoxic effects of different isolates could rationally be used to employ them for the management of the mahogany borer.     

杨扇舟蛾幼虫高毒力绿僵菌菌株筛选研究

测定了21株绿僵菌的平板单菌落生长和产孢量情况,证明(Ma1,Ma42,Ma788,Ma789)4菌株产孢量高,通过对这4株绿僵菌菌株的不同温度水浴处理,发现了孢子的萌发条件;并开展了4菌株对杨扇舟蛾幼虫的毒力测定,筛选出Ma789菌株的致病力较强。     

昆虫生长调节剂和绿僵菌对意大利蝗几丁质酶活力的影响

[目的]探明3种昆虫生长调节剂(苯氧威、氟虫脲、灭幼脲)和一种生物农药(绿僵菌189菌株)对意大利蝗3龄蝗蝻几丁质酶活力的影响.[方法]用药剂处理3龄意大利蝗蝗蝻,用分光光度法测定几丁质酶分解几丁质产物N-乙酰氨基葡萄糖的含量.[结果]3种昆虫生长调节剂浓度达到50mg/L时对几丁质酶的抑制作用超过200％;绿僵菌189菌株对意大利蝗几丁质酶活力也有一定程度的抑制,且抑制作用随着孢子浓度的增加而增大,当孢子浓度达到5×107时,意大利蝗几丁质酶的活力仅为3.8U.[结论]昆虫生长调节剂和绿僵菌均抑制意大利蝗几丁质酶活力.     

绿僵菌防治黄脊竹蝗试验

通过绿僵菌粉剂和绿僵菌油悬浮剂与杀虫双对黄脊竹蝗的防治对比试验，结果表明：绿僵菌能有效地控制黄脊竹蝗的虫口密度，对防治3龄前跳蝻效果最好，11d后达防治高峰，且对林间天敌和植被无毒害作用，是一种环保有效的生物农药。