全文获取类型
收费全文 | 4900篇 |
免费 | 207篇 |
国内免费 | 307篇 |
专业分类
林业 | 335篇 |
农学 | 295篇 |
基础科学 | 149篇 |
1377篇 | |
综合类 | 1669篇 |
农作物 | 227篇 |
水产渔业 | 259篇 |
畜牧兽医 | 635篇 |
园艺 | 306篇 |
植物保护 | 162篇 |
出版年
2024年 | 20篇 |
2023年 | 50篇 |
2022年 | 93篇 |
2021年 | 114篇 |
2020年 | 131篇 |
2019年 | 147篇 |
2018年 | 99篇 |
2017年 | 183篇 |
2016年 | 223篇 |
2015年 | 194篇 |
2014年 | 218篇 |
2013年 | 315篇 |
2012年 | 357篇 |
2011年 | 403篇 |
2010年 | 336篇 |
2009年 | 316篇 |
2008年 | 269篇 |
2007年 | 308篇 |
2006年 | 276篇 |
2005年 | 193篇 |
2004年 | 170篇 |
2003年 | 135篇 |
2002年 | 87篇 |
2001年 | 93篇 |
2000年 | 93篇 |
1999年 | 75篇 |
1998年 | 56篇 |
1997年 | 72篇 |
1996年 | 65篇 |
1995年 | 55篇 |
1994年 | 57篇 |
1993年 | 29篇 |
1992年 | 34篇 |
1991年 | 32篇 |
1990年 | 26篇 |
1989年 | 26篇 |
1988年 | 16篇 |
1987年 | 9篇 |
1986年 | 10篇 |
1985年 | 11篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有5414条查询结果,搜索用时 15 毫秒
101.
嗜热毛壳菌纤维素酶对小尾寒羊瘤胃代谢的影响 总被引:1,自引:0,他引:1
本试验研究不同水平嗜热毛壳菌纤维素酶对小尾寒羊瘤胃代谢的影响。试验动物为4只安装有永久性瘤胃瘘管的成年小尾寒羊公羊(平均体重为45 kg),采用4×4拉丁方试验设计,在基础日粮中分别添加0%、0.3%、0.6%和0.9%4个水平酶制剂,采集瘤胃液测pH值、氨氮浓度和挥发性脂肪酸(VFA)浓度。试验结果表明:各组试羊瘤胃液平均氨氮浓度的变化范围为10.01-12.80 mg/dL,各组之间相同时间点差异不显著(P>0.05)。瘤胃液平均pH值在6.50-6.80范围内变动,试验组pH值低于对照组(P<0.05),以0.6%水平较好。各组试羊瘤胃乙酸、丙酸及总 VFA浓度的变化规律基本相同,即喂料后逐渐上升,其中乙酸和总VFA浓度在2 h后达到最高点,丙酸浓度在4 h 达到最高点,随后平稳下降于饲喂前降至最低点,再次采食后又重复出现此规律。试验组瘤胃乙酸和总VFA浓度显著或极显著高于对照组(P<0.05或P<0.01),以0.6%水平组最高。 相似文献
102.
103.
The present study focuses on hybridization program involving two species belonging to two different vandaceous genera, viz., Ascocentrum ampullaceum (Roxb.) Schltr. var. auranticum, a narrow endemic orchid of Manipur and Vanda coerulea Griff., an endangered orchid of Appendix I of CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora), to synthesize the primary hybrid genus with intermediate and improved characters in the F1 generation. Observations on the crossability in the present bigeneric cross between V. coerulea and A. auranticum had been achieved with 60% success when V. coerulea was taken as female parent. Murashige and Skoog (MS) basal medium at half-strength was effective for the development of the hybrid seedlings of V. coerulea × A. auranticum followed by Vacin and Went (VW) and Knudson C (KC) media. The best response of seedling growth was observed on MS medium at half-strength supplemented with 2.3 μM kinetin + 0.5 μM α-naphthalene acetic acid with maximum shoot height (2.7 cm), leaf number (4.6) and root number (4.1) after 150 days of inoculation. The survival percentage and growth performance of the seedlings were found to be higher (80% survival) in potting substrate consisting of brick:charcoal in the ratio 2:1 mulched with moss (Sphagnum sp.) than in potting substrate consisting of brick:charcoal:tree fern in the ratio 2:1:1. The first flowering was observed in the hybrid seedlings of V. coerulea × A. auranticum after 2 years of transfer to the ex vitro environment. Morphologically, the flowers differed from that of the parents clearly showing the success of the hybridization experiment. Registration of the hybrid has been made with the Royal Horticultural Society with the nomenclature Ascocenda ‘Kangla’ (No. T128725). 相似文献
104.
AIM: To investigate the feasibility to inhibit the expression of MHCⅡ by special siRNA targeting class Ⅱ major histocompatibility complex (MHC Ⅱ) transactivator (CⅡTA), which might regulate MHC Ⅱexpression for suppressing immune rejection. METHODS: Five different siRNA were designed, synthesized and transfected into freshly isolated rat corneal keratocytes. At 24 hours posttransfection, the changes of MHC Ⅱexpression were detected by flowcytometry, and the mRNA abundance of CⅡTA and MHC Ⅱ was measured by FQ-PCR after inducing with recombinant rat interferon-gamma (IFN-γ). RESULTS: Different siRNA showed different reduction in MHC Ⅱ and CⅡTA expression compared with the control (P<0.01). Among the five groups, the siRNA-4 was the most efficient. The mRNA content of CⅡTA and MHC Ⅱ were reduced by 95.10%±1.25% and 82.70%±1.95% respectively and the expression of MHC Ⅱ was inhibited by over 80% in siRNA-4 group at 24 hours posttransfection. CONCLUSIONS: The special siRNA targeting to CⅡTA inhibits CⅡTA mRNA and further inhibits its regulation of MHC Ⅱ molecular expression. The blockade of MHC Ⅱ by siRNA may be useful for further studying allogeneic corneal limbal transplantation. 相似文献
105.
AIM:To investigate the effect of protein kinase C on resistin expression in 3T3-L1 adipocytes.METHODS:The differentiated 3T3-L1 adipocytes were incubated with 50 nmol/L phorbol 12-myristate 13-acetate (PMA) or 5 μmol/L Ro-31-8220 for 24 h.Expression of resistin mRNA was detected by RT-PCR and expression of resistin protein was detected by Western blotting.RESULTS:Compared with control,PMA increased the expression of resistin mRNA and protein in 3T3-L1 adipocytes significantly (P<0.01),while Ro-31-8220 decreased the expression of resistin mRNA and protein in 3T3-L1 adipocytes obviously (P<0.01).CONCLUSION:Protein kinase C signal pathway may regulate resistin expression in 3T3-L1 adipocytes. 相似文献
106.
107.
转rolA、B、C基因枳橙快繁技术 总被引:5,自引:0,他引:5
通过对转rol基因枳橙B、D、E系及对照的高接植株春梢茎段进行萌芽诱导、增殖及试管苗生根成苗的试验,摸索出适宜枳橙快繁各种培养基配方及培养条件。结果表明,以MS+6-BA1mg/L的培养基适合于芽萌发,以MS+6-BA0.5mg/L+NAA0.05mg/L的培养基适合于芽增殖,生根则以1/2MS+NAA0.5mg/L+0.2%活性炭的组合最佳,转基因各系生根率均在93.3%以上,显著高于对照生根率(66.7%)。快繁苗移栽成活率可达90%,目前生长良好。 相似文献
108.
109.
Asheesh Shanker Aarti BhargavaRicha Bajpai Stuti SinghSucharita Srivastava Vinay Sharma 《Scientia Horticulturae》2007
Characterization of microsatellites is extremely important for the development of molecular markers. Here, we present the detection and abundance of microsatellites or simple sequence repeats (SSRs) in UniGene sequences of Citrus sinensis. A total of 427 SSRs were mined in 8786 UniGene sequences downloaded from National Center for Biotechnology Information (NCBI). Depending on the repeat units, the length of SSRs ranged from 14 to 21 for mono-, 14 to 48 for di-, 18 to 48 for tri-, 24 to 40 for tetra- and 42 bp for hexa-nucleotide repeats. Average density of SSRs (1SSR/12.92 kb of 5518.71 kb sequences mined) suggests that only 4.43% of sequences contained SSRs. Di-nucleotide repeats were most frequent repeat type (49.41%) followed by tri-nucleotide repeats (41.45%). An attempt was made to design primer pairs for 427 identified SSRs but these were found only for 216 sequences. The positions of SSRs with respect to open reading frame (ORF) detected and annotation of sequences containing SSRs were also carried out to assign function to each of the sequences. 相似文献
110.
ZHANG Shui-jun ZHU Sheng-xing LI Jie MA Xiu-xian FENG Liu-shun FAN Zheng-jun 《园艺学报》2007,23(8):1480-1483
AIM:To investigate how brain-dead state affects the heart structure and function and the effect of PKC-α in BA-Ma mini pigs.METHODS:Ten Ba-Ma mini pigs were randomized into 2 groups: brain-dead group (n=5),and control group (n=5). The brain-dead model was made by increasing intracranial pressure,while the control group was maintained anesthesia for 24 h. The concentrations of cTnT,TNF-α,IL-1β and IL-6 in serum were determined at 6,12 and 24 h after brain death. At 24 h,heart tissues were observed by HE staining and electron microscope. The expression of PKC-α was detected by immunohistochemistry and RT-PCR.RESULTS:(1) Histological changes of myocardium: flaky bleeding under endocardium and dissolution of myocardium were found in optical microscope. In electron microscope dropsical mitochondria and confluent muscle fiber were found. (2) Changes of serum cTnT: serum cTnT for brain-dead group began to increase gradually since 6 h,and were significantly higher at each time point than those in control group (P<0.05). (3) Changes of inflammatory factors: IL-1β,IL-6,and TNF-α in brain-dead group began to increase gradually since 6 h,and were significantly higher at each time point than those in control group (P<0.05). (4) Changes of PKC-α expression: PKC-α mRNA and protein expressions in brain-dead group increased significantly at 24 h (P<0.05).CONCLUSION:Brain death may evoke heart structure and functional injury,and increase the levels of inflammatory factors and PKC-α. The activation of PKC-α may participate in the process of heart injury. 相似文献