缺磷胁迫下番茄侧根形成与miR164及NAC1表达的关系

为了研究番茄幼苗在缺磷胁迫下根系形态发育与生长素、生长素信号转导途径中的转录因子NAC1,以及调控NAC1 表达的上游miR164之间的关系。试验以5和500 μmol/L磷浓度作为缺磷胁迫和对照,检测了外源生长素NAA（1-naphthalene acetic acid）及生长素抑制剂NPA（N-1-naphthylphthalamic acid）对侧根形成的影响; 同时采用RT-PCR检测了NAC1和miR164在缺磷胁迫下的时序表达。结果表明,缺磷胁迫下侧根大量形成与生长素及其运输密切相关,在侧根迅速形成的24 h内,NAC1的表达在缺磷胁迫下增强; 而其上游的miR164表达降低,从而揭示了缺磷胁迫下侧根形成与miR164调节NAC1表达之间的关系。     

蔓荆短枝容器育苗试验初报

本试验探索了蔓荆育苗的新技术,利用蔓荆耐风耐沙耐盐碱的习性,在沿海沙荒、沙滩、风口地段种植,长势良好。蔓荆是沿海防风固沙的一种有效植被,引种蔓荆,是一条经济实用、本小利大的开发利用沙荒沙滩的新路子,为营造沿海防风固沙林开辟了一条行之有效的途径,为改善沿海地区的生态环境创造了条件。     

柳枝浸提液对小麦籽粒发芽的抑制作用研究

研究了柳树枝叶浸提液对小麦籽粒发芽的抑制效应，结果表明：抑制作用的大小随浸提时间和浸提液浓度的不同而异，煎煮时间对小麦种子发芽抑制强度依次为4h〉3h〉2h〉0．5h，浸提液不同浓度的抑制效果为280g／L〉200g／L〉120g／L〉40g／L。     

Quantitative trait loci for adventitious and lateral roots in rice

The mass of a root is determined by two underlying morphogenetic factors: (1) the length of the main root axis (root axis length: RAL), and (2) the amount of lateral root development generated by a unit length of the axis (branching index: BI). The mass of a rice adventitious root was partitioned into these two factors at the quantitative trait locus (QTL) level, using ‘Akihikari’ (a lowland cultivar as a recurrent parent) × ‘IRAT 109’ (an upland cultivar) backcross inbred lines. Four QTLs were detected for the dry weight of individual adventitious roots (root dry weight: RDW), which were associated with a QTL for RAL (chromosomes 1 and 9) or a QTL for BI (chromosomes 6 and 11). For BI, an additional QTL was detected on chromosome 1. The absence of co‐located QTLs suggested that RAL and BI are under separate genetic control. Despite its small population size and few replications, this study suggests that the partitioning of root mass into these underlying components could be useful for understanding the complexity involved in the genetic control of root growth.     

Effects of the Adaptable Distribution for Lateral Forces Considering modes Coupling on Structural Pushover Analysis

Two high and flexible RC frame structures are analyzed;the paper explains the rise in the structural damping ratio and the base mode's drifting,and the modes coupling is caused by the greatly rising damping ratio.The paper also proposes that the modes coupling need be considered in the pushover analysis of the high and flexible RC frame structures.A CQC adaptive distribution for lateral forces in considering the modes coupling at every load step is given,and is compared with the traditional SRSS one.Meanwhile,some practical engineering suggestions are given.     

甘蓝型油菜株高、第一分枝高和分枝数的QTL检测及候选基因筛选

株高、分枝数及第1分枝高是油菜重要的农艺性状。本研究利用甘蓝型油菜GH06和P174杂交,F2通过单粒法连续自交至F11构建重组自交系群体,利用油菜60K芯片对该群体进行基因分型,构建高密度遗传连锁图谱。结果表明,该图谱包含2795个SNP多态性标记位点,总长1832.9 c M,相邻标记间平均距离为0.66 c M。在此图谱基础上采用复合区间作图法(CIM),检测到3个农艺性状的24个QTL。其中11个株高QTL分别位于A01、A06、A07、A08、A10和C06染色体,单个QTL解释5.00%~15.26%的表型变异;7个第1分枝高QTL分别位于A06、C05和C06染色体,单个QTL解释5.04%~12.99%的表型变异;6个分枝数QTL分别位于A03、A07、C01、C04和C06染色体,单个QTL解释5.95%~8.14%的表型变异。将156个拟南芥株高相关基因、10个拟南芥第1分枝高相关基因和148个拟南芥分枝数相关基因与QTL对应置信区间序列进行同源比较分析(E1E–20),分别找出了20个株高候选基因、3个第1分枝高候选基因以及12个分枝数候选基因。2个环境中在A07染色体上重复检测到的QTL置信区间检测到与株高相关的候选基因ATGID1B/GID1B和WRI1,A08染色体上重复检测到的QTL置信区间检测到SLR/IAA14和AXR2/IAA72个与株高相关的候选基因。在具有部分置信区间重叠的q2013FBH-C05-1和q2014FBH-C05-2区间均检测到第1分枝高候选基因PHT1;8,在A03和C06染色体上的QTL置信区间内,分别检测到4个分枝数候选基因,匹配E值介于0~3E–56之间。     

DYNAMIC ANALYSIS OF ASYMMETRIC FRAME-SHEAR WALL SYSTEM BY STAGE CONTINUITY METHOD

The lateral structures of frame-shear wall system are simplified into bending beams and shear beams which stiffness can vary in different stages along the building height. The three-dimension stiffness matrix and mass matrix are deduced, and the characteristic equation is established to calculate the tortuous free vibration of the asymmetric system. The method is simple and has satisfactory precision, and it is easily fulfilled in micro-computers.     

桑枝在食用菌方面应用试验初报

在含白糖、石膏各1％,分别以桑枝粒,桑枝屑和蚕沙为基础,加与不加麦麸,或以蚕沙代替麦麸的不同8种菌种培养基上培养,除加入0.5％尿素的明显表现不良影响外,其余均能正常生长,尤以在蚕沙培养基中生长最快。表明蚕沙可以代替麦麸使用。桑枝屑袋栽毛木耳,一潮耳片的生物学效率为47.8％～60.8％。凤尾菇在含10％麦麸桑枝培养基上的生物学效率是64.2％,在100％桑枝屑是58.8％,在含桑枝屑30％、桑枝粒 70％是58.2％,在含稻草 60％与40％的桑枝屑生料袋栽时生物学效率分别是43.3％与31.7％,表明加入稻草混合反而效果不好。     

Effects of soil bulk density on seminal and lateral roots of young maize plants (Zea mays L.)

It is well established that increasing soil bulk density (SBD) above some threshold value reduces plant root growth and thus may reduce water and nutrient acquisition. However, formation and elongation of maize seminal roots and first order lateral (FOL) roots in various soil layers under the influence of SBD has not been documented. Two studies were conducted on a loamy sand soil at SBD ranging from 1.25 g cm^–3 to 1.66 g cm^–3. Rhizotrons with a soil layer 7 mm thick were used and pre‐germinated plants were grown for 15 days. Over the range of SBD tested, the shoot growth was not influenced whereas total root length was reduced by 30 % with increasing SBD. Absolute growth rate of seminal roots was highest in the top soil layer and decreased with increasing distance from the surface. Increasing SBD amplified this effect by 20 % and 50 % for the top soil layer and lower soil layers, respectively. At the end of the experiment, total seminal roots attributed to approximately 15 % of the total plant root length. Increasing SBD reduced seminal root growth in the lowest soil layer only, whereas FOL root length decreased with SBD in all but the uppermost soil layer. For FOL, there was a positive interaction of SBD with distance from the soil surface. Both, increasing SBD and soil depth reduced root length by a reduction of number of FOL roots formed while the length of individual FOL roots was not influenced. Hence, increasing SBD may reduce spatial access to nutrients and water by (i) reducing seminal root development in deeper soil layers, aggravated by (ii) the reduction of the number of FOL roots that originate from these seminal roots.     

FSY30型林木枝丫切碎机试验分析

FSY30型林木枝丫切碎机是林区林木剩余物碎化处理的专用设备,该机采用圆盘式切碎装置,结构简单实用,解决了我国现有林木剩余物碎化处理设备野外作业性能差、切削效率低和原料适应面窄等问题,为我国林区人工林迹地整理更新、抚育和采伐等作业产生的各种林木剩余物碎化处理提供了适用的设备。该机性能和生产试验结果表明：其作业效率比现有机型提高20%以上,作业成本减少15%以上,具有显著的经济效益和一定的生态效益。