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AIM: To investigate the preventive effects of Shengmai San (SMS) on oxidative damage in mentally stressed mice.METHODS: An oxidative stress mouse model was established by moustache-removed. Protein carbonyl and thiobarbituric acid reactive substance (TBARS) formation were determined as the oxidative stress markers.RESULTS: (1)Moustache-cut was founded to significantly enhance the behavioral movements of mice, especially large movements (movement 2 and rearing). SMS pre-administration inhibited the accelerated movements. (2) Protein carbonyl was increased in brain, heart, liver and kidney. TBARS in liver and heart increased in the moustache-cut mice, but SMS pretreatment inhibited the increased protein carbonyl and TBARS.CONCLUSION: SMS has the preventive effects on oxidative damage induced by emotional stress. 相似文献
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AIM: To explore the effect of the pretreatment of hypertonic saline (HTS) in hepatic ischemia reperfusion (I/R) injury.METHODS: The rats were divided into sham group (sham group), ischemia reperfusion group (IR group) and pretreatment of hypertonic saline group (HTS group). Partial hepatic ischemia reperfusion model was used. The rats were sacrificed at the time of 1 h, 3 h, 6 h, 12 h and 24 h after reperfusion in each group, respectively. Blood samples were obtained to examine ALT. The expression of the CD11b/CD18 (Mac-1) on the neutrophils was analyzed by flow cytometry. RT-PCR and Western blotting were used to examine the expression of intercellular adhesion molecule-1 (ICAM-1) in livers and chromatometry was performed to detect the activity of myeloperoxidase (MPO) in livers. The morphology of hepatocytes and the structure of sinusoid were observed by histological examinations. RESULTS: ① HTS pretreatment decreased the level of ALT at the time points of 3 h, 6 h and 12 h after reperfusion (P<0.05). ② Mac-1 expression in HTS group was lower at 6 h and 12 h after reperfusion compared with IR group (P<0.05). ③ MPO activity in HTS group was lower at 6 h, 12 h and 24 h compared with IR group (P<0.05). ④ RT-PCR and Western blotting analysis indicated that the pretreatment of HTS inhibited the expression of ICAM-1 in livers after reperfusion. ⑤ Moderate hepatocyte swelling and few neutrophil infiltration were observed in HTS group.CONCLUSION: Pretreatment with HTS has the effect on hepatic ischemia reperfusion injury by inhibiting the expression of Mac-1 on circulating neutrophils and the expression of ICAM-1 in the liver. 相似文献
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AIM: To explore the changes of excitatory amino acids and N-methyl-D-aspartate receptor (NMDAR) in brain undergoing ischemia-reperfusion injury following hepatic portal occlusion (HPO) in vivo.METHODS: The electrolytes and pH of portal vein blood,the content of Glu and Asp in brain,and the expression of NMDAR mRNA were studied with blood-gas analysis,HPLC and semi-quantitative RT-PCR method in two groups(HPO or sham),respectively.Three time points(6 h,12 h and 24h after reperfusion) were included.RESULTS: HPLC measure showed that the content of cortex Glu and Asp in group HPO elevated significantly after reperfusion compared with sham group, the peaks were at 6 h and 24 h [Glu:(349±145) μg·g-1wt,(456±203) μg·g-1wt vs (238±24) μg·g-1wt,(225±59) μg·g-1wt;Asp:(134±50) μg·g-1wt,(166±50) μg·g-1wt vs (99±24) μg·g-1wt,(71±20) μg·g-1wt].Moreover,semi-quantitative RT-PCR analysis discovered that the expression of NMDAR mRNA increased significantly ,the subunit NR1 mRNA was higher in reperfusion 6 h and last to 12 h(1.63±0.06 vs 1.18±0.05;1.73±0.06 vs 1.17±0.03),the peak of NR2B mRNA was in 12 h (1.75±0.04 vs 1.18±0.05) ,but they did not further increase after reperfusion for 24 h.CONCLUSION: HPO increases the content of Glu and Asp and the expression of NMDAR in brain cortex.The Glu-NMDAR pathway plays a role in the mechanism for the brain injury after HPO.The products of IR injury might be the key factor to cause EAAs acumulation in synapse space. 相似文献
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AIM:To investigate the mechanisms by which bilirubin inhibits acute lung injury (ALI). METHODS:30 female Wistar rats were divided into normal group, ALI group and bilirubin treatment group. ALI was induced by intravenous injection of LPS. The contents of OH-, H2O2 and O2· in the lung as well as the expression of caspase-3 in the lungs were investigated. RESULTS:(1) The contents of OH-, H2O2 and O2· in the lung homogenate and the expression of caspase-3 in the lungs in ALI group increased compared with those in normal group (P<0.05). (2) The contents of OH-, H2O2 and O2· in the lung homogenate and the expression of caspase-3 in the lungs in bilirubin treatment group increased compared with those in normal group, but decreased compared with those in ALI models (P<0.05). CONCLUSION:(1) Bilirubin was shown to be able to ameliorate apoptosis in ALI rats. (2) The increase in the contents of OH-, H2O2, O2· in ALI group indicated the development of oxidative lung injury, which was ameliorated by bilirubin. (3) Expression of caspase-3 confirmed that the model made by LPS was associated with apoptosis, which was reduced by bilirubin. 相似文献
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AIM: To evaluate the therapeutic efficacy of intratracheal instillation of porcine pulmonary surfactant (PPS) in rats with lipopolysaccharides (LPS)-induced early-stage ALI in this study.METHODS: SD rats weighing 200 g-300 g were randomly divided into 4 groups: LPS (1.5 mg·kg-1)+saline,LPS+PPS 100 mg·kg-1,LPS+PPS 150 mg·kg-1,LPS+PPS 200 mg·kg-1.The PaO2 and PaCO2,as well as survival rate of rats were examined for 6 h after the start of PPS-instillation.Then,rats were killed and lungs were immediately removed for lung index (LI) and histological analysis.The bronchoalveolar lavage fluid (BALF) was collected for measurement of total protein (TP) contents,TNF-α level and white blood cell(WBC) numbers.RESULTS: Significantly increased PaO2,reduced mortality rate,decreased total protein and TNF-α contents in BAL,as well as lung index and meliorated histological appearance were observed in three PPS-treated groups compared with group given saline after LPS (P<0.05).The therapeutic effect in PPS150 and PPS200 groups was better than that in PPS100 group.CONCLUSION: Intratracheal PPS instillation provides protective effect on acute lung injury in rats induced by LPS. 相似文献
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新疆棉花生长中期低温冷害指标的初步研究 总被引:8,自引:0,他引:8
分析不同棉区严重气候减产年的地膜棉花中期发育进程、气象条件以及夏季(6~8月)冷害年对新疆棉花产量的影响.结果表明:当石河子棉区6月平均气温距平≤-0.5 ℃,阿克苏、喀什棉区5~6月气温持续偏低且月气温距平均≤-0.5 ℃时,棉花的开花日期滞后,其中喀什棉区棉花开花期比常年晚5天以上、石河子棉区和阿克苏棉区晚3天以上,这就出现了一般气候减产年.或当6~8月中至少有2个月的月气温为负距平、且6~8月的平均气温距平在0~-0.4 ℃之间时,3大棉区中近70%的年份可能出现一般气候减产年;而当6~8月的温度距平均为负值,且其平均气温距平≤-0.5 ℃时,3大棉区70%以上的年份可能出现严重的气候减产年. 相似文献