Frequency of symptomatic trees removal in small citrus blocks on citrus huanglongbing epidemics

Among the recommended measures for citrus Huanglongbing (HLB) management, the systematic elimination of symptomatic trees is the most argued and difficult to be accepted and accomplished by citrus growers. Elimination of recently affected HLB trees represents a short term yield loss and cost increase due to the need of frequent inspections and removal operations. This work aimed to evaluate the effect of different frequencies of inoculum reduction applied at individual citrus blocks scale (or local inoculum reduction) on HLB temporal progress. Eight experiments were carried out in new planted and older citrus blocks with 504–1290 trees/plot. In all experiments, inspections to detect symptomatic trees were done in a fortnightly or monthly frequency. The treatments of frequencies of local inoculum reduction varied from fortnightly to 6 months. Annual disease progress rate was estimated by logistic model for each plot. No difference on HLB progress rates among treatments was observed, except in experiments 1 and 3 where less frequent tree removal resulted in higher disease progress rate. This ineffectiveness of local inoculum reduction on the disease progress rate was explained by the higher weight of primary spread on HLB epidemics than the secondary spread within plots associated with small size and narrow shape of treated plots (except for experiments 1 and 3), high dispersal capacity of HLB-insect vector among plots and groves, and strong control of psyllid within the plots (except for experiment 1, with poor insecticide spray program). Also, the high amount of inspections to detect symptomatic trees before the eradication treatment, which reduced the escapes (asymptomatic and non visual detectable diseased trees) contributed for these results. It is important to note that these results were obtained with only small citrus plots (0.8–2.9 ha) and they cannot be extended to larger groves and farms amenable to HLB management by the symptomatic tree removal and vector control.     

大豆油分含量SSR标记及QTL定位

大豆油分含量是数量遗传性状。为了选育高油品种,有效利用高油基因和转基因研究,并使其广泛应用于育种和生产实践,通过构建群体,利用分子标记手段对油分基因在基因图谱上的位置进行了标记,结果表明,标记Satt491和油分含量相关,定位于E连锁群上。     

小型猪特异性麻醉颉颃剂对大鼠脑NO/cGMP信号转导系统的动态影响

为探讨NO/cGMP信号转导系统对小型猪特异性麻醉颉颃剂催醒分子机理的调控,144只Wistar大鼠随机分为对照组和试验组,对照组和试验组再分为早期催醒组、中期催醒组和晚期催醒组。用分光光度法测定脑NOS活性和NO产量,ELISA测定脑cGMP含量。结果显示,小型猪特异性麻醉颉颃剂能明显地激活大鼠相关脑区的NOS活性和NO及cGMP产量,并且大鼠不同脑区NOS活性、NO产量和cGMP含量的变化趋势不仅与大鼠行为学变化相平行,还与不同时期表现出的催醒效果基本吻合。这表明小型猪特异性麻醉颉颃剂的催醒可能与激活特定脑区的NO/cGMP信号转导系统相关。     

选择与适应:兰科植物查尔酮合酶基因家族成员的分子进化和功能趋异

回顾了近十五年来国内外对各科植物查尔酮合酶基因CHS分子进化的研究成果,并结合作者近年来对兰科植物CHS基因分子进化的研究结果,对兰科植物CHS基因分子进化和功能趋异的机制展开综述,揭示了植物中普遍存在的CHS基因分子进化以及伴随的功能趋异的现象和机制,初步探讨了CHS基因分子进化与生物进化和自然选择的关系。     

高效凝胶过滤色谱法测定肽的相对分子量分布

采用高效凝胶过滤色谱法,对肽粉、酵母核苷酸和自溶酵母粉三种肽类产品的相对分子量大小及分布进行了测定。结果表明,此三种产品的相对分子量分布范围集中在2000Da以下,寡肽含量丰富,其中含有一定量的游离氨基酸和氨基酸残基。该方法简便快捷、准确性高、重复性好,适合肽类产品分子量(<1 0KD)分布的分析检测。     

RAPD技术及其在农作物遗传育种中的应用

论述了RAPD技术的原理、意义及其在基因定位、遗传图谱的构建、亲缘关系和系统进化的研究、品种纯度的鉴定、体细胞杂种鉴定和目标基因的早期鉴定等方面应用于农作物育种的情况。由于RAPD技术简捷快速、花费少,虽有一定的局限性,但仍在基因组研究中发挥着不可替代的重要作用。     

Surveys of cereal diseases and pests in the Netherlands. 2. Stem-base diseases of winter wheat

In the period 1974–1986, ca. 100 commercial winter wheat fields were surveyed annually for stembase diseases. In these years, on average 6% of the tillers was infected with eyespot in spring at the first-second, node stage. Eyespot intensity in spring was high in years with high temperature during winter. Eyespot, intensity in the milky-ripe stage in July, averaged 13% of the culms and was high in years with high temperature in April and high precipitation in March, April and May. These data may improve forecasts.In the milky-ripe stage, 2% of the culms were infected with sharp eyespot, but its intensity has gradually increased during the survey years. No significant correlation of sharp eyespot intensity with dry periods in autumn, spring or summer was found.Symptoms of take-all were found on 0.2% of the stem-bases during ripening. Its intensity was low in years with high precipitation in March, April, May and June and high temperature in May and June.Samenvatting Een honderdtal percelen wintertarwe werd in 1974–86 jaarlijks op voetziekten geïnventariseerd. In het voorjaar, tijdens het, eerste en tweede knoopstadium van het gewas, was gemiddeld 6% van de spruiten aangetast door oogvlekkenziekte. De intensiteit van de ziekte was hoog in jaren met een hoge gemiddelde wintertemperatuur. Tijdens het melkrijpe stadium, in juli was gemiddeld 13% van de halmen aangetast door oogvlekkenziekte. De aantasting in juli was hoog in jaren met een hoge temperatuur in april en veel neerslag in de maanden maart, april en mei. Met deze gegevens kunnen adviessystemen worden verbeterd.Scherpe oogvlekkenziekte was op gemiddeld 2% van de halmen in juli aanwezig. De ziekte nam geleidelijk met de jaren toe. De jaarlijkse intensiteit was niet gecorreleerd met droge perioden in de herfst, voorjaar of zomer.Symptomen van halmdoder waren op gemiddeld 0.2% van de halmen aanwezig. De intensiteit van de ziekte was hoog in jaren met weinig neerslag in maart, april, mei en juni en met een lage temperatuur in mei en juni.     

An advisory model for control of Puccinia recondita in winter wheat

Epidemics ofPuccinia recondita and resulting yield loss of winter wheat were studied in field experiments over three seasons in the Netherlands. Results are reported and used to construct an advisory model for control of brown rust, based on rust monitoring. If the fraction of leaves with rust (I) at a certain development stage is determined, the average number of rust sori per leaf (M) was estimated by: M=EXP(1.84+1.39ln(ln[1/(1-I)]). The final number of sori per leaf (Mf) at early dough was forecast by an exponential growth: Mf=M·EXP(RGR·t). The relative growth rate (RGR) averaged 0.163/day and the forecasting period, t, until early dough, was derived from published data. The forecast number of sori-days per leaf (S, AUDPC-value) was then obtained by: S=(Mf-M)/RGR. Yield loss (kg/ha) by brown rust was 1.15 times the number of sori-days per leaf at low rust intensities. The efficacy of the fungicides used was 85%. The forecast avoidable yield loss (L, kg/ha) was calculated by: L=0.85 (1.15 S). Economic thresholds for brown rust control at different development stages are given for Dutch wheat fields at a cost level of 270 kg/ha for one fungicide application.