全文获取类型
收费全文 | 1688篇 |
免费 | 196篇 |
国内免费 | 167篇 |
专业分类
林业 | 7篇 |
农学 | 36篇 |
基础科学 | 2篇 |
14篇 | |
综合类 | 428篇 |
农作物 | 29篇 |
水产渔业 | 456篇 |
畜牧兽医 | 1019篇 |
园艺 | 12篇 |
植物保护 | 48篇 |
出版年
2024年 | 5篇 |
2023年 | 30篇 |
2022年 | 65篇 |
2021年 | 87篇 |
2020年 | 97篇 |
2019年 | 132篇 |
2018年 | 53篇 |
2017年 | 111篇 |
2016年 | 87篇 |
2015年 | 81篇 |
2014年 | 95篇 |
2013年 | 79篇 |
2012年 | 120篇 |
2011年 | 139篇 |
2010年 | 98篇 |
2009年 | 107篇 |
2008年 | 91篇 |
2007年 | 90篇 |
2006年 | 116篇 |
2005年 | 65篇 |
2004年 | 55篇 |
2003年 | 36篇 |
2002年 | 43篇 |
2001年 | 21篇 |
2000年 | 19篇 |
1999年 | 14篇 |
1998年 | 13篇 |
1997年 | 13篇 |
1996年 | 16篇 |
1995年 | 9篇 |
1994年 | 10篇 |
1993年 | 4篇 |
1992年 | 7篇 |
1991年 | 1篇 |
1990年 | 7篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1980年 | 9篇 |
1979年 | 6篇 |
1978年 | 1篇 |
1975年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有2051条查询结果,搜索用时 15 毫秒
141.
142.
B. C. Njau 《Veterinary research communications》1990,14(2):123-128
The secondary antibody response to sheep red blood cells and bovine serum albumin (BSA) was measured in rabbits infested with adult Rhipicephalus evertsi evertsi. The response was reduced (particularly for BSA) but still displayed anamnestic characteristics. Resistance against ixodid ticks associated with antibodies detected by gel diffusion and enzyme linked immunosorbent assay techniques early in the primary challenge was acquired by the immunized hosts only. This suggests that a non-specifically activated immune system enables hosts to develop rapid resistance against tick parasitism. 相似文献
143.
144.
K.P. Flaming R.C. Thaler F. Blecha J.L. Nelssen 《Comparative immunology, microbiology and infectious diseases》1988,11(3-4):181-187
Mitogen-stimulated lymphocyte proliferation, delayed-type hypersensitivity (DTH) reactions, interleukin-2 (IL-2) production, and growth performance were evaluated in 3-week-old pigs treated with imuthiol. Lymphocyte proliferative responses to Con A and PWM were reduced (P < 0.05) in pigs treated with imuthiol at 25 mg/kg; PHA proliferative responses were not influenced by imuthiol treatment. Imuthiol at 2.5 mg/kg and 25 mg/kg lowered IL-2 production when compared to saline-treated controls. Delayed-type hypersensitivity responses to PHA were higher in 25 mg/kg imuthiol-treated pigs; however, 2.5 mg/kg imuthiol-treated pigs had lower DHT reactions. Imuthiol at 2.5 mg/kg and 25 mg/kg reduced (P < 0.05) average daily feed intake. These data suggest that in vivo imuthiol treatment in pigs lowers lymphocyte proliferative responses, IL-2 production, and growth performance. 相似文献
145.
Stanley G Rabinowitz Jayashree Huprikar Caroline Whitacre 《Comparative immunology, microbiology and infectious diseases》1979,1(4):295-303
C57B16 mice were immunized with either live, attenuated TC-83 strain VEE virus vaccine or formalin-inactivated VEE vaccine combined with Bordetella pertussis. The kinetics of specific donor and adoptively-immunized recipient anti-VEE neutralizing antibody responses were studied. Donor mice immunized with either live or inactivated VEE virus vaccine combined with potent adjuvants develop specific anti-VEE IgM and IgG responses as early as 7 days post-immunization. Anti-VEE IgM antibody responses comprise the majority of anti-VEE neutralizing antibody at this early time period. By 14 to 21 days post-immunization, anti-VEE IgG responses predominated. When adoptively-immunized recipients were studied, the anti-VEE IgM to IgG predominance seen in donors early after administration was reversed, and for each time-period studied, recipients' serum anti-VEE antibody class responses consisted principally of IgG rather than IgM antibody. Since T-cells cooperation with B-cells is critical in the IgM-IgG antibody shift, these studies support the critical role T-cells exert in adoptive transfer in a murine model of experimental VEE infection. Furthermore, immunization with either live or inactivated VEE vaccine coupled to a potent adjuvant induce comparable donor and adoptively-immunized recipient anti-VEE antibody class responses. 相似文献
146.
G.S Elissalde Harland W Renshaw James A Walberg 《Comparative immunology, microbiology and infectious diseases》1980,3(4):433-445
A review of the recent and/or significant literature concerning Corynebacterium equi, including its morphologic, biochemical, immunological, and pathological characteristics in the foal, humans and other animals is presented. The similarity in the tissue responses of mammalian hosts to C. equi and Mycobacterium spp. is discussed.
The antigenic structure and virulence factors associated with C. equi. other corynebacteria and mycobacteria are compared.
The immunological aspects of resistance to C. equi are considered. The evidence suggests that the major immune response elicited in the foal by C. equi is cell-mediated. However, the immunopathogenic mechanism needs clarification. Areas of future research are suggested. 相似文献
147.
Fat globule membrane of sow milk as a target for adhesion of K88-positive Escherichia coli 总被引:1,自引:0,他引:1
F Atroshi T Alaviuhkola R Schildt M Sandholm 《Comparative immunology, microbiology and infectious diseases》1983,6(3):235-245
Membrane adhesion of K88-positive Escherichia coli was studied on intestinal brush-border membranes on 237 Finnish Landrace pigs. Forty-one per cent of the brush-border membrane preparations aggregated E. coli (positive adhesion). Similar dualism of adherence/nonadherence was observed on sow milk fat globule membranes. Washed milk fat globules (washed cream) can be used as a convenient source of material for adhesion studies. Bacterial adherence on to milk fat globules is evident as agglutination of the globules (dark-field microscopy). By this procedure the sows can be typed according to their receptor phenotype. This simple principle of fat globule agglutination due to receptors for K88-positive E. coli might be complicated by SigA-mediated bacterial adherence. Fat globule membranes were shown to contain SigA, which may act as a mediator of bacterial adherence onto fat globules. The significance of this adhesive property of milk fat globule might be to provide alternative receptors for E. coli thus preventing bacterial adhesion on to gastro-intestinal epithelium of the offspring. Sow milk fat globules can be used for typing E. coli for membrane adhesiveness. The adhesiveness of the strains showed a good correlation with the presence of the K88 antigen, as well as the hydrophobicity of the bacterial strain as determined by an association on Phenyl-Sepharose beads. 相似文献
148.
中华硬蜱唾液腺抗原成分的分析研究 总被引:8,自引:0,他引:8
中华硬蜱唾液腺提取物(SGE)经SDS聚丙烯酰胺凝胶电泳显示出24条蛋白带,其中主带有6条,分子量分别为142、105、94、66、64和56KD。用中华硬蜱叮刺家兔后血清做免疫印渍,特异性抗原带为105和94KD。说明中华硬蜱105和94KD的蜱唾液腺蛋白原能够有效地诱慢宿主产生抗体。 相似文献
149.
接种泰氏锥虫及其抗原使家兔和小鼠产生抗伊氏锥虫的交叉免疫力 总被引:2,自引:0,他引:2
用培养的泰氏锥虫制备的死、活两种虫体抗原,接种于家兔和小鼠,一定时间后用伊氏锥虫强毒株攻击,观察其血清抗体的变化及交叉免疫保护能力。试验表明,泰氏锥虫虫体抗原可刺激机体产生较高的体液抗体;免疫动物有一定的抵御伊氏锥虫攻击的能力,与对照组相比,免疫动物血液中虫体出现的时间较迟,最初的数量少,临床症状轻。死虫抗原组的交叉免疫保护能力较活虫抗原组强。 相似文献
150.
Swine fever. Immunisation of piglets 总被引:4,自引:0,他引:4
P Precausta F Kato A Brun 《Comparative immunology, microbiology and infectious diseases》1983,6(4):281-289
Vaccination against Swine Fever using the CL Chinese strain can be done in 7-day-old piglets if they are born of non-immune sows. The simultaneous weaning and vaccination emphasises the safety of this strain. The excellent immunity observed confirms the immunocompetence of 7-day-old piglets. In piglets born of immune sows and also weaned at 7 days, passive protection can extend beyond the age of 2 months if the sow is vaccinated several months prior to gestation. The immune level of the piglets would seem to depend on the interval between vaccination of the sow and farrowing and can be attributed to the quality of the antibodies transmitted by the colostrum. Piglets born of sows vaccinated 10 months prior to farrowing can be vaccinated as early as 5 weeks; the protection percentage observed at the age of about 6 months is over 80%. A booster injection at this age then confers immunity to future breeders throughout their economic life, i.e. 4 years in the reported experiment. 相似文献