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排序方式: 共有471条查询结果,搜索用时 15 毫秒
31.
新城疫病毒融合蛋白裂解位点基因的分离克隆及在原核细胞中的表达 总被引:5,自引:1,他引:4
用SPF鸡胚繁殖新城疫病毒(NDV)F46E9株(强毒)、LaSotaE4株(弱毒),对病毒进行纯化,抽提RNA。用1对均为27个碱基的引物进行RT-PCR,扩增出了2个毒株的融合蛋白裂解位点(Fc)基因。将Fc基因定向克隆入pUC18的EcoRI和SalⅠ位点之间,获得2个毒株Fc基因克隆pUCF46Fc和pUCLaFc,用EcoRI/SalⅠ双酶切法、PstⅠ单酶切法、PCR法和核酸探针法对其进行了鉴定。将鉴定好的LaSotaE4Fc基因定向导入表达载体质粒pBV221,获得重组子pBVLaFc。PCR和内切酶酶切法鉴定表明,Fc插入的位置和方向都正确无误。用E.coliDH5α通过热诱导法进行了表达。用SDS-PAGE和Western-blot法检测了表达产物。结果表明,有1条能够与NDV多抗反应的特异条带,分子量约15700,与预期大小一致,说明是Fc基因的表达产物 相似文献
32.
兽药磺胺二甲嘧啶在土壤中的生态行为 总被引:10,自引:0,他引:10
通过室内培养和土壤薄层层析等实验方法,研究了磺胺二甲嘧啶对土壤微生物的毒性及其在土壤中的降解、迁移特性。结果显示:在土壤磺胺二甲嘧啶浓度为1mg kg^-1时,土壤中细菌和真菌数量明显减少(p〈0.05),但随时间延长,该抑制作用变缓;磺胺二甲嘧啶在土壤中降解缓慢,持留时间久,在灭菌土和未灭菌土中的半衰期分别为223.9d和102.4d,并容易在土壤中迁移和渗漏,迁移系数R大于0.7,迁移和渗漏速度受介质pH的影响,在非中性环境下,移动和渗漏能力增强。表明磺胺二甲嘧啶是一种在土壤中不易被降解、容易迁移和渗漏到水体、对土壤微生物和水体产生危害的新型污染源。 相似文献
33.
《兽医药理学》实验教学是《兽医药理学》教学过程中的重要组成部分,其教学效果直接影响该专业整体教学质量的高低。总结了《兽医药理学》实验教学改革过程中的几点经验。 相似文献
34.
Wylie CE Collins SN Verheyen KL Richard Newton J 《Veterinary journal (London, England : 1997)》2011,189(3):248-256
Equine laminitis is a highly debilitating disease of the foot. Despite its perceived importance, epidemiological characteristics are poorly understood and the true frequency of the disease remains unclear. The objective of this study was to retrospectively assess previous research to identify publications which provide the best evidence of the frequency of naturally-occurring equine laminitis. A systematic review of English language publications was conducted using MEDLINE (1950–2010), CAB Direct (1910–2010) and IVIS (1997–2010). Additional publications were included by searching bibliographies. Search terms included laminitis, equine, frequency, prevalence and incidence. Studies that allowed frequency estimations to be made for naturally-occurring equine laminitis were included. Information was extracted using predefined data fields, including 13 study quality indicators.Sixty-nine publications were appraised. Ten were considered to provide the most reliable information, estimating the frequency of equine laminitis ranging from 1.5% to 34%. Previous publications estimating laminitis frequency were generally poor quality. Laminitis frequency varied across publications however the publications included in this review focussed on many of the different underlying laminitis aetiologies and comparison of the frequencies between groups would be inappropriate. High-quality evidence-based studies are needed to estimate the true disease frequency in different equine populations. 相似文献
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38.
本文介绍了一个实用的零售药店管理信息系统,针对目前药店管理信息系统的现状,把GSP理念引入到管理信息系统中.实践证明该系统具有良好的性能和较强的实用性. 相似文献
39.
SR92A系黄羽肉鸡与萧山鸡及其杂种后代的DNA指纹分析 总被引:2,自引:0,他引:2
本文以EAV(禽内源性反转录病毒片段 )为探针 ,EcoRI为限制性内切酶 ,对萧山鸡、SR92A系及其杂交后代进行了DNA指纹图谱的研究。结果表明 :EAV探针适合于鸡的DNA指纹分析 ,能产生清晰可辩的图谱。平均条带总数为11.384± 0 .84 4 ,两品种间、公母间条带总数均无显著差异。萧山鸡和SR92A系品种内相似系数分别为 0 .5 81、0 .5 11,高于品种间相似系数 (0 .2 31)。杂交后代相似系数为 0 .30 1。 相似文献
40.
Isolation of Pasteurella haemolytica and correlation with serum antibody response in clinically normal beef calves 总被引:1,自引:0,他引:1
Bacteria from the nasal cavity and trachea were cultured, and serum antibody titers determined for Pasteurella haemolytica serotype 1 in 164 beef calves obtained from a closed herd on range pasture. At the first sampling, P. haemolytica serotype 1 was cultured from 16.4% of the calves. Antibody titers were determined by a quantitative fluorimetric method and the mean titer was 9.5 +/- 5.8. Fifty-seven randomly selected calves were used to study the correlation of serum antibody response and positive culture of P. haemolytica under natural conditions. Clinical signs of respiratory disease were not observed in those calves. During the observation periods, there was a two-fold increase in the percentage of calves that were culture positive. There was no significant difference between mean serum antibody titers or frequency distribution of antibody titers from the two samplings. Comparisons between serum antibody titers, rise in titers, and P. haemolytica isolation failed to reveal any significant correlation. Of the 9 calves that had a decline in antibody titer to P. haemolytica, none was culture positive. Seroconversion to respiratory viruses did not correlate with P. haemolytica related variables. 相似文献