储粮害虫磷化氢抗性分子遗传学研究进展

由于长期单一使用磷化氢,储粮害虫对其产生的抗药性日益增强,已成为威胁粮食安全储藏的重大问题。自从发现两个磷化氢抗性基因rph1和rph2,以及完成赤拟谷盗全基因测序以来,储粮害虫磷化氢抗性基因的相关研究迅速发展。近年来,在抗性基因的鉴定、表达、进化、突变等研究方面取得了一定成果,为储粮害虫磷化氢抗性治理提供了新的科学指导。本文就储粮害虫磷化氢抗性分子遗传学的研究进展进行综述。     

Effects of different NS genes of avian influenza viruses and amino acid changes on pathogenicity of recombinant A/Puerto Rico/8/34 viruses

To examine the effects of the NS1 and NEP genes of avian influenza viruses (AIVs) on pathogenicity in mice, we generated recombinant PR8 viruses containing 3 different NS genes of AIVs. In contrast to the reverse genetics-generated PR8 (rPR8) strain and other recombinant viruses, the recombinant virus rPR8-NS(0028), which contained the NS gene of A/chicken/KBNP-0028/2000 (H9N2) (0028), was non-pathogenic to mice. The novel single mutations of 0028 NS1 to corresponding amino acid of PR8 NS1, G139D and S151T increased the pathogenicity of rPR8-NS(0028). The replacement of the PL motifs (EPEV or RSEV) of pathogenic recombinant viruses with that of 0028 (GSEV) did not reduce the pathogenicity of the viruses. However, a recombinant virus with an EPEV-grafted 0028 NS gene was more pathogenic than rPR8-NS(0028) but less than rPR8. The lower pathogenicity of rPR8-NS(0028) might be associated with the lower virus titer and IFN-β level in the lungs of infected mice, and be attributed to G139, S151 and GSEV-PL motif of NS1 gene of 0028. In conclusion we defined new amino acid residues of NS1 related to mice pathogenicity and the presence of pathogenic NS genes among low pathogenic AIVs may encourage continuous monitoring of their mammalian pathogenicity.     

Genetic diversity of the pine pathogen Lecanosticta acicola in Slovenia and Croatia

Brown spot needle blight (BSNB), a disease of pine trees caused by the fungus Lecanosticta acicola, has been known in Slovenia since 2008 and in Croatia since 1975. Recent outbreaks in Slovenia prompted this study to compare L. acicola populations in these two neighbouring European countries. Sixty-nine isolates collected from three pine species (Pinus mugo, P. halepensis and P. nigra) were used to determine the phylogenetic relationships, genetic structure, and reproductive strategy of the pathogen. EF1-α sequences showed that Slovenian and Croatian isolates share a common ancestry with individuals from central and northern Europe. Population structure analysis revealed four distinct population clusters of L. acicola in these two countries, generally corresponding to their respective geographic location and host. An unequal ratio of mating types and a low overall genetic diversity in the population indicated a strong influence of asexual reproduction. Although some of the oldest recorded European occurrences of BSNB are from Croatia, this study provided no evidence that the population studied in Croatia was the source of the sampled outbreaks in Slovenia. Recent outbreaks of L. acicola in Slovenia are most likely due to introductions from other, yet to be identified, sources.     

From regionally predictable to locally complex population structure in a freshwater top predator: river systems are not always the unit of connectivity in Northern Pike Esox lucius

Contemporary genetic diversity is the product of both historical and contemporary forces, such as climatic and geological processes affecting range distribution and continuously moulded by evolutionary forces selection, gene flow and genetic drift. Predatory freshwater fishes, such as Northern Pike Esox lucius, commonly exhibit small population sizes, and several local populations are considered endangered. Pike inhabit diverse habitat types, including lakes, rivers and brackish marine waters, thus spanning from small isolated patches to large open marine systems. However, pike population structure from local to regional scales is relatively poorly described, in spite of its significance to developing conservation measures. We analysed microsatellite variation in a total of 1185 North European pike from 46 samples collected across both local and regional scales, as well as over time, to address two overarching questions: Is pike population structure associated with local and/or regional connectivity patterns, and which factors likely have the main influence on the contemporary distribution of genetic diversity? To answer this, we combined estimators of population diversity and structure to assess evidence of whether populations within (i) habitats, (ii) drainage systems and (iii) geographical regions are closer related than among these ranges, and whether patterns are temporally stable. Contrasting previous predictions that genetic drift obscures signals of postglacial colonisation history, we identified clear regional differences in population genetic signatures, suggesting a major effect of drainage divides on colonisation history and connectivity. However, several populations deviated from the general pattern, showing that local processes may be complex and need to be examined case‐by‐case.     

植物种群构件的理论概述及研究进展

综述了植物种群构件的理论概况和国内外的研究现状以及对我国种群构件的研究展望.文中指出构件理论的思想依据、形态依据、生理依据和构件理论的五项基本内容.同时,论述了近30余年来,包括植物花构件、叶构件、芽构件、枝构件、茎构件、根构件等在内的植物种群构件研究现状.我国植物种群构件研究要注意构件间的相互作用以及构件与环境间的相互作用;注意与生理生态相结合;研究对象多元化,为开发和保护我国植物资源提供科学依据,将构件研究成果转化为生产力.     

油松大蚜的种群动态、有效积温及防治

通过野外调查和室内不同温度下饲养观察,对油松大蚜(Cinara formosana)生活史、种群动态、有效积温、天敌、物候预测和化学防治进行了研究。结果表明:油松大蚜1年可发生8代,6月中旬分化出有翅孤雌蚜并扩散,10月上旬性蚜交配产卵,以卵越冬;油松大蚜种群数量1年内有2个高峰期,分别在6月上旬和9月上旬,8月上旬—8月中旬种群数量最低;油松大蚜不同虫态的发生与山桃(Prunus davidiana)、连翘(Forsythia suspense)、龙爪槐(Sophora japonica)和火炬树(Rhus typhina)的一些物候相吻合,可通过观察这些植物的物候来预测油松大蚜各虫态发生情况;油松大蚜优势天敌依次为异色瓢虫(Leis axyridis)、七星瓢虫(Coccinella septempunctata)、蚜茧蜂(未鉴定);油松大蚜4个龄期的若虫及全世代的发育起点温度分别为4.5、6.7、5.9、7.7、3.1℃,有效积温分别为48.6、49.3、67.4、62.7、291.7日·度;胶带-药膜法防治油松大蚜效果显著,14 d后处理区校正虫口减退率可达80%以上。     

新城市乡村关系建构与地域圈域化发展选择

对我国城乡关系处理的现状进行了概述,在借鉴日本与韩国地区地域圈域均衡发展的经验的基础上,研究了我国圈域化运作对城乡统筹的影响,即构筑经济循环与物质循环圈域,完善城乡统筹下的地域城市。