Cellulase Supplementation Does Not Improve the Digestibility of a High-Forage Diet in Horses

Six mature Arabian geldings were used in a two-period crossover study to investigate the effects of cellulase supplementation on fiber digestion. Horses were randomly assigned to either a control (CO; n = 3) or a cellulase (CE; n = 3) treatment for the first period and then treatments were switched for period 2. Each period consisted of a 10-day diet adaptation followed by a 3-day total fecal collection. The enzyme mixture contained 40,000 cellulase units/g and was fed at a rate of 3 g/day split evenly between two feedings. During the diet adaptation period, horses had ad libitum access to timothy hay and were also fed 165 g whole oats as a carrier for the supplement. When eating the CO treatment, horses consumed 16% more hay than when on the CE treatment (P = .004). Fecal output also tended to be greater when horses consumed the CO treatment as compared with CE treatment (P = .07). No differences were found between treatments for fecal percent dry matter (DM%), fecal neutral detergent fiber (NDF), fecal acid detergent fiber (ADF), fecal nitrogen (N), or fecal gross energy (GE). There was a trend for horses consuming the CO treatment to digest more NDF than when consuming the CE treatment (34.6% ± 1.5 vs 31% ± 1.5; P = .07). Horses also digested a greater %ADF, %N, and Mcal of energy when consuming the CO treatment than when consuming the CE treatment (P < .05). Cellulase addition to a hay-based horse diet decreased digestion of fiber components.     

Effect of feeding fermentable fiber on synthesis of total and mucosal protein in the intestine of the growing pig

In a previous study, a reduced efficiency of ileal digestible threonine (THR) use for body protein deposition was observed in growing pigs when pectin was included in the diet. This response was not due to increased physical endogenous ileal THR loss. Our aim was to explore the contribution of diet-induced increases in protein synthesis in the colon, especially mucins, to dietary THR requirements. Twelve barrows (21 kg mean BW) were fed either a cornstarch–soybean meal-based diet (Control) or Control with 12% pectin (Pectin). Pigs were given intravenously 1.5 mmol/kg BW of L-1-¹³C valine (40 mol%) to measure fractional and absolute synthesis rates (FSR, ASR, respectively) of mucosal and whole intestinal protein in the jejunum and colon. Dietary pectin inclusion increased plasma levels of glucose, isoleucine and glutamine (P < 0.05) but had no effect on insulin or urea nitrogen (P > 0.10). There were no differences in FSR and ASR of whole intestinal protein in jejunum and colon (P > 0.10). The FSR of mucosal proteins in colon, not in jejunum, was increased with dietary pectin supplementation (P < 0.05). Assuming mucosal protein mass is constant, these results imply that the higher protein synthesis in colon mucosa contributes to the reduced THR efficiency observed in pectin-supplemented diet.     

棉花WOX转录因子家族基因的全基因组鉴定与分析

为了挖掘可用于棉花花器官或纤维发育研究的候选基因,基于已发布的陆地棉全基因组数据,利用生物信息学的分析方法鉴定了陆地棉WOX(WUSCHEL-related homeobox,WOX)转录因子基因家族,并从染色体分布、基因及蛋白理化性质、蛋白结构、多重序列比对、系统进化树和基因表达模式等方面对该基因家族的特征进行了比较分析。结果表明,鉴定到37个陆地棉WOX转录因子家族基因,命名为GhWOX1~GhWOX37。亚基因组定位结果显示,37个陆地棉WOX转录因子家族基因分布在除了A04、A06、A09、D04、D06和D09号亚基因组以外的20个亚基因组,A亚组比D亚组多1个GhWOX转录因子家族基因。多重序列比对分析棉花WOX转录因子家族成员均具有高度保守的同源异型结构域;系统进化树分析发现棉花WOX转录因子家族可以分为3个亚家族(Ⅰ类、Ⅱ类和Ⅲ类),各亚类分别含有23,7,7个GhWOX转录因子家族基因成员。对NCBI中陆地棉的EST数据库比对分析,有11个GhWOX转录因子家族成员没有可匹配的EST序列,其他26个GhWOX转录因子家族成员在棉花的根、茎、叶、花、胚珠、纤维和种子等组织和器官中广泛表达。为深入研究棉花和其他植物中WOX家族的鉴定和功能研究奠定了基础。     

豌豆双重标记新突变基因fcu的染色体定位

对豌豆隐性突变体带化茎伞状花序(Fasciatedculm and umbel) 的遗传特性进行了研究。扫描电镜研究表明, 突变体的花蕾密生于茎顶端, 花序呈伞状。遗传试验表明, F1 代植株皆具正常的茎和花序, F2 代突变性状和正常标记性状呈3∶1 分离, 表明该突变性状由单隐性基因支配, 用基因符号fcu 表示。fcu 基因具有使茎带化和产生伞状花序的双重遗传效应。以标志基因系L1238 和w2 为工具材料, 对豌豆fcu 突变体进行了染色体定位。结果表明, 突变基因fcu 与矮茎基因le 表现连锁, 其交换值为31 .92% ±3 .62% 。已知le 位于第IVB 连锁群,因而推定fcu 也在该连锁群上。     

河北省转基因抗虫棉花品种纤维品质分析

从2004年开始河北省棉花品种审定进入了除短季棉品种之外全部为转基因抗虫棉品种的新阶段,5年间共审定转基因抗虫棉花品种65个,本研究对其纤维品质性状进行了分析。结果表明:上半部平均长度集中在27.1~32.7 mm,86.2%品种属于Ⅱ型;比强度集中在24.6~32.0 cN/tex,53.85%品种属于Ⅲ型,没有Ⅰ型品种,20%品种低于Ⅲ型;马克隆值集中在3.6~5.3,13.84%品种属于Ⅰ型,83.08%品种属于Ⅱ型。2004-2007年,纤维品质逐年变好,但由于2007年吐絮期长时间降雨,造成2008年纤维品质出现了下滑。总的说来,我省转基因抗虫棉品种没有上半部平均长度≥31 mm,比强度≥34 cN/tex,马克隆值在3.7~4.2的综合表现Ⅰ型的品种,主要表现在比强度没有Ⅰ型品种,因而,我们的应该加强培育高比强的棉花新品种。     

利用微米木纤维定向重组技术形成超高强度纤维板的细胞裂解理论研究

采用人造板微观力学和木材细胞学理论，提出了一种利用定向重组微米木纤维技术形成超高强度人造板的细胞裂解理论和强度参数预测方法。应用提出的理论，可以根据纤维、木质素、细胞直径和排列的程度，建立理想状态下微米重组高强度定向纤维板木纤维细胞在定向重组微米技术形成超高强度人造板的细胞裂解理论，为人造板力学运用数学手段深入到细胞结构研究的深度提供了一种新的理论和方法。     

鹅掌楸种源材性遗传变异与选择

在鹅掌楸两个种及其杂种多地点７年生种源试验林中,随机抽取３块试验林共２８２株样本,分别遗行木材基本密度（ＢＤ）和纤维长度（ＦＬ）的测定分析,在分布型拟合与精度分析的基础上,进行一般线性方差模型分析,结果为：中国鹅掌楸种源间ＢＤ和ＦＬ均差异显著（a=0.05),５个北美鹅掌楸种源间ＢＤ和ＦＬ未达到显著差异,地点间差异及种源与地点的交互作用不显著,平均值Ｔ检测表明中国掌楸与北美鹅掌楸两种间ＢＤ和ＦＬ均不存在显著差异,鹅掌楸的ＢＤ总均值为0.397g/cm^3,ＦＬ总均值达１.603mm,属于中小密度,长纤维或近长纤维树种,差异分析揭示了种内差异是掌释属的主要变异来源,种内种源间存在选择的遗传基础,性状遗传相关分析认为,鹅掌楸ＢＤ和ＦＬ之间相互不密切（r=0.157),两本材料状与各生长因子间相关亦不密切,说明他们之间基因连锁不紧密,可独立选择。BD种源广义遗传力为H^2BD=0.65,受遗传控制较强,ＦＬ为Ｈ^FL=0.29,受遗传控制中偏弱,两性状年－年间遗传相关密切,在上述分析结果的基础上方３种标准进行了鹅掌楸属材料种源选择,其中,在“强度选择”条件下,选出ＢＤ优良的种源为庐山和桑植,ＦＬ优良的种源为浏阳和赣武夷,均为中国掌楸种源,北美种源虽然与中国种源在总体上不存在明显差异,但多数处于中等水平上,对照杂种夫性系ＢＤ和ＦＬ的均超过鹅掌楸性状总均值,但未超过最好的种源。     

闽南山地桉树纤维材优良无性系的选择研究

对福建漳州岩溪、天马 2个试验点 1 3个桉树纤维材无性系的生长性状、木材纤维形态和化学组分等性能进行测定与分析 ,并经多性状综合评判 ,选择出具有较高的生长量、生物量和纤维产量 ,适合闽南山地发展的C9、C1 0、EC1、EC3、C6共 5个纤维材优良无性系 ,其中尤以C9、C1 0、EC1三个无性系的各性状为最优。这 5个无性系 ,3a生年平均树高和胸径生长量、每株干材重量和纤维产量分别可达 3 93～ 6 60m、3 83～ 4 97cm ,9 71～ 2 0 48kg·a- 1 和 4 3 6～ 9 5 2kg·a- 1 ,而且木材纤维形态和化学组成等特性良好 ,可进一步扩大繁殖、推广应用。     

纤维性质对成纸性能的影响

对纤维的三个基本性质－长度、强度和粗度对纸性的影响进行了综述，着重讨论了对强度性质的影响。     

国内外竹材造纸及其经济效益评价

本文根据国内外(包括中国、印度、日本、孟加拉国、缅甸、菲律宾、澳大利亚、苏联等)报道的竹材制浆造纸方面资料综合而成。文章内容有引言、竹材纤维形态与化学成分、世界竹材造纸工业简况和造纸竹材利用的经济效益。文章列举大量事实说明造纸工业用竹材为原料比用针叶材或阔叶材经济效益高。