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51.
YAN Xiao-cheng MU Wei-na QIANG Ye ZHAO Hui-chen SUN Qi YAO Xiao-min ZHANG Yu-chao LIU Yuan-tao 《园艺学报》2000,36(9):1661-1666
AIM To investigate the effects of cytochrome P450 (CYP450) epoxygenase/epoxyeicosatrienoic acid (EET) pathway on insulin resistance in obese mice, and to explore the possible mechanisms. METHODS High-fat diet-induced obesity model was established in C57BL/6Cnc mice, and the obese mice were randomly divided into 3 groups, including obesity group (treated with saline; n =10), EET group (treated with 11,12-EET; n =10) and EET inhibitor 14,15-epoxyeicosa-5(Z)-enoic acid (EEZE) group (n =10). Normal C57BL/6Cnc mice (n =10) treated with saline served as control. Protein expression of CYP2J2 (one of CYP450 epoxygenases) and hypoxia-inducible factor-1α (HIF-1α) was measured by Western blot. Vessel-like structure was detected by immunofluorescence staining. The serum levels of insulin, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and monocyte chemoattractant protein-1 (MCP-1) were measured by ELISA. RESULTS In obese mice, homeostasis model assessment of insulin resistance (HOMA-IR) values were increased, the protein level of CYP2J2 was reduced, and the protein level of HIF-1α was increased in adipose tissues as compared with the controls (P <0.05). The serum levels of MCP-1, IL-1β, IL-6 and TNF-α were also significantly increased in obese mice (P <0.05). After treatment with 11, 12-EET, the HOMA-IR values were decreased compared with vehicle-treated obese mice, HIF-1α expression levels were decreased in the adipose tissue, and the serum levels of MCP-1, IL-1β, IL-6 and TNF-α were reduced (P <0.05). Immunohistochemical results of adipose tissue from vehicle-treated obese mice showed a marked decrease in vessel-like structures (CD31-positive) compared with normal control mice (P <0.05). EET treatment significantly increased the newly formed vessel-like structures in the visceral adipose tissues of obese mice as compared with vehicle-treated obese mice (P <0.05). CONCLUSION High-fat diet-induced obesity and insulin resistance are closely related to the CYP450 pathway. Exogenous EETs effectively decrease obesity-induced insulin resistance possibly through pro-angiogenesis and attenuation of hypoxia and inflammation. 相似文献
52.
GUAN Cang-hai ZHAO Yu-qiao Guo Liang CHEN Yu-Zhu WANG Wei-Na JIANG Xing-ming 《园艺学报》2000,36(10):1908-1912
MNX1 antisense RNA 1 (MNX1-AS1) is a newly discovered long non-coding RNA (lncRNA), which is highly dysregulated in various carcinomas and its expression level is closely related to the overall survival and prognosis of patients. MNX1-AS1 regulates the occurrence and development of carcinomas by endogenous competitive adsorption of miRNA, regulating cell cycle, inducing epithelial mesenchymal transformation and activating multiple signaling pathways. The in-depth study of the carcinogenesis of MNX1-AS1 is useful for the early diagnosis, targeted therapy and prognostic assessment of relevant carcinomas. This article reviews the roles of MNX1-AS1 in malignant tumor. 相似文献
53.
LUO Ming-hao HU Shu-peng WANG Rui-yu Li Chang Lü Ding-yi YANG Xi-yang LUO Su-xin 《园艺学报》2000,36(10):1739-1744
AIM To investigate whether interleukin-1β (IL-1β) regulates endothelial nitric oxide synthase (eNOS) phosphorylation at Ser1177 site in human umbilical vein endothelial cells (HUVECs), and to explore its possible mechanism. METHODS The HUVECs were randomly divided into normal control group, tumor necrosis factor-α (TNF-α) group, IL-1β group, IL-6 group, SC79 [protein kinase B (PKB/AKT) specific agonist] group and SC79+IL-1β group. Western blot was used to determine the protein levels of eNOS, p-eNOS-Ser1177, AKT and p-AKT-Ser473 in the HUVECs. Chemical colorimetry was used to detect the nitric oxide (NO) content in the culture medium of HUVECs. RESULTS No statistically significant difference of p-eNOS-Ser1177 level in HUVECs treated with TNF-α and IL-6 was observed as compared with normal control group (P >0.05), while the protein level of p-eNOS-Ser1177 in the HUVECs and the content of NO in the culture medium of HUVECs decreased significantly in IL-1β group (P <0.05), and the protein level of p-AKT-Ser473 in the HUVECs was decreased as compared with normal control group (P <0.05). The AKT agonist SC79 blocked the down-regulation effect of IL-1β on p-eNOS-Ser1177 level in the HUVECs and NO content in the culture medium of HUVECs (P< 0.05). CONCLUSION IL-1β down-regulates the protein level of p-eNOS-Ser1177 in HUVECs and affects the activity of eNOS, which may be involved in AKT/eNOS signaling pathway. 相似文献
54.
辣椒新品种辣优4号的选育 总被引:2,自引:0,他引:2
辣优 4号是以湖南地方品种分离株Ⅱ经多代自交筛选纯化的优良自交系 33号椒为母本、以材料h7 1经多代单株自交筛选的优良自交系 30号椒为父本配制而成的辣味型一代杂种 ,熟性早 ,前期产量比对照品种湘研 1号增产 16 .5%~ 4 9.1%。一般产量 16 0 0~ 2 80 0kg·(6 6 7m2 ) - 1。田间表现抗疫病、青枯病和病毒病。结果性好 ,单果质量 35~ 50g ,果实长牛角形 ,果表面光滑 ,色绿 ,商品率高 ,品质优良。现已在广东、广西、江西、海南、湖南等地推广种植 ,面积达80 0hm2 。 相似文献
55.
辣椒新品种平椒3号的选育 总被引:3,自引:0,他引:3
平椒3号是以自交系99101为母本,自交系9949为父本配制的早熟一代杂种。果实羊角形,青熟果深绿色,果纵径17.7cm,果肩径3.0cm,果肉厚0.25cm,平均单果质量29.5g,肉质脆嫩,辣味浓且有香味,商品性好。田间对疫病、炭疽病、病毒病的抗性明显优于猪大肠。露地栽培每667m2青椒产量为3700kg左右,适宜西北地区及四川、山西、湖南等嗜辣地区露地栽培。 相似文献
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59.
平阳霉素对盆栽小菊意大利红的诱变效应 总被引:3,自引:0,他引:3
利用1、2、4、8 mg·L-1平阳霉素(PYM)对小菊品种意大利红离体培养的叶片和茎段进行诱变处理.结果表明,PYM对叶片和茎段愈伤组织的诱导和分化具有明显的抑制作用,随着PYM质量浓度的增加,愈伤组织的诱导和分化均呈明显的下降趋势.对茎段外植体经不同质量浓度PYM处理后的M1代,进行田间主要观赏性状观察和统计分析,发现诱变后代与对照相比,平均株高、冠幅、节间长度和叶片长/宽均减小,花径增大,管状花数目和舌状花数目增加.诱变后代中还出现了叶色、叶型、花色以及花型变异的植株.随着PYM质量浓度由低到高,花型的变异率分别为0、2.5%、3.3%和1.2%,花型多为舌状花向下翻卷,或舌状花前端变尖;花色的变异率分别为0、4.9%、8.2%和1.2%,花色均往浅色方向变异.PYM最适的诱变质量浓度为2~4 mg·L-1. 相似文献
60.
Umberto MOLINI Vimanuka MUTJAVIKUA Mari de VILLIERS Lourens de VILLIERS Alaster SAMKANGE Lauren M. COETZEE Siegfried KHAISEB Giovanni CATTOLI William G. DUNDON 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(5):707
Samples from eleven birds (chicken, dove and peacock) with symptoms of fowlpox, caused by the avipoxvirus (APV), were collected in seven different areas of the Windhoek district, Namibia between April and October 2021. A fragment of the 4b core protein and the DNA polymerase gene of APV were amplified by PCR from the DNA of the samples and sequenced. Phylogenetic analysis revealed that the viruses present in the chickens all belonged to clade A1 while the viruses in the doves and peacock were from subclade A3.1. This is the first report of subclade A3.1 avipoxvirus in peacock. In addition, all of the samples obtained from chickens were shown by PCR to be positive for the integration of reticuloendotheliosis virus while those from the doves and peacocks were negative. This study is the first characterization of avipoxvirus in Namibia and provides additional information on the presence of avipoxvirus in southern Africa. 相似文献