Pleuritis in slaughter pigs: Relations between lung lesions and bacteriology in 10 herds with high pleuritis

Pleuritis in slaughter pigs has increased in recent years in the Netherlands. The aim of the present study was to determine what respiratory pathogens were involved in pleuritis.In total, lungs of 968 slaughter pigs from 10 herds with high prevalence of pleuritis were morphologically examined for size, location, and type of lesions. Moreover, histology and bacteriology were performed.Examination of gross lung lesions showed 45% pleuritis, 14% pleuropneumonia and 38% catarrhal pneumonia. Peribronchiolar cuffing was found in 61 of 142 samples. Actinobacillus pleuropneumoniae was cultured from 22 lung samples from four herds. Pasteurella multocida was cultured from 55 lung samples in eight herds. No specific pattern with respect to the causal pathogens was found.In conclusion, no single infectious cause of pleuritis was found. A variety of infectious agents combined with environmental factors should be considered as a cause of pleuritis.     

我国部分地区牛支原体肺炎和关节炎的病原体诊断

重庆等4省市从外省引进的肉牛群发生以严重肺炎和关节炎为主要表现的疾病。该病的病变主要集中于肺脏,其组织学变化主要是间质增生,纤维素渗出,干酪样坏死,淋巴细胞、嗜中性粒细胞和单核细胞浸润。PCR检测病牛肺脏显示牛支原体阳性,而丝状支原体丝状亚种、牛分支杆菌和多杀性巴氏杆菌为阴性。从肺等组织中分离到牛支原体以及大肠杆菌、沙门氏菌、链球菌、葡萄球菌和烟曲霉,没有分离到巴氏杆菌。结果显示本病是以牛支原体感染为主引起的牛支原体肺炎和关节炎,长途运输等应激因素是该病突发的重要诱因,其他细菌和/或真菌继发感染加重了病情。     

山羊中绵羊肺炎支原体的分离及鉴定

从四川省乐至县发生胸膜肺炎性传染病的山羊群中采集12个鼻拭子及4个肺组织病料,进行病原分离培养和特异性PCR检测。结果从9个鼻拭子和4个肺组织中分离到支原体,经鉴定均为绵羊肺炎支原体,未发现丝状支原体簇成员及多杀性巴氏杆菌和溶血性曼氏杆菌。结果表明,绵羊肺炎支原体是引起该山羊群发生胸膜肺炎的病原,同时说明绵羊肺炎支原体也是山羊支原体性肺炎的重要病原之一。     

青海地区丝状支原体山羊亚种的流行病学调查

为了解丝状支原体山羊亚种在青海地区的流行状况,利用丝状支原体山羊亚种抗体检测正向间接血凝试剂盒对2007～2009年采自青海各地区的1 200份山羊血清进行检测。结果显示:不同地区山羊血清的阳性率从5.0%～21.0%不等,平均阳性率为16.1%。从季节上看,冬春季节发病率要高于夏秋季节。青海地区的丝状支原体山羊亚种的感染率偏高,应该加强对由丝状支原体山羊亚种的防控。     

种公牛感染温氏枝原体后的生殖健康检测研究（英文）

[目的]研究种公牛感染温氏枝原体后的生殖健康。[方法]通过血常规和生殖健康检测等方法对药物治疗前后种公牛的血液和精液品质进行试验研究。[结果]在未给予磷酸伯氨喹治疗前,病牛有轻度的贫血,血液涂片中可见大量的温氏枝原体;病牛阴囊壁肿大,睾丸变软,精液品质下降,精子活力降低,初级和次级畸形的精子比例增大;药物治疗后,温氏枝原体很快从血液中消失,临床症状逐渐缓解,顺利通过了1个月后生殖健康检测。[结论]该研究为进一步研究温氏枝原体与公牛生殖机能下降之间的关系奠定了基础。     

绵羊肺炎支原体 p113 基因的序列分析及功能预测

采用多种软件对p113基因的相似性、跨膜结构、重复序列、信号肽、抗原表位等进行预测,并与同源序列进行比较分析.结果表明,p113基因是猪肺炎支原体黏附素基因p97的直系同源基因,并具有与P97蛋白R2区类似的特征性重复序列.通过综合比较分析,推测P113蛋白极可能是绵羊肺炎支原体的黏附素和膜表面免疫原.     

复方硫氰酸红霉素可溶性粉对人工感染鸡慢性呼吸道病的疗效试验

用复方硫氰酸红霉素可溶性粉以每升水中加0.5、0.6、0.7、0.8、0.9 g 5个剂量对人工感染鸡慢性呼吸道病的鸡群混饮治疗,同时设立硫氰酸红霉素可溶性粉对照组.试验结果表明,用药5d后,复方硫氰酸红霉素可溶性粉每升水加0.6、0.7、0.8、0.9g剂量组的有效率和治愈率皆高于硫氰酸红霉素可溶性粉组.     

Outbreak of bovine clinical mastitis caused by Mycoplasma bovis in a North Italian herd

This report describes an outbreak of Mycoplasma bovis mastitis affecting 45 cows in a herd of 122 dairy cattle in Northern Italy. Clinically, the outbreak was characterized by agalactia, multiple swollen and painless quarters, high milk somatic cell count and unresponsiveness to conventional antibiotic therapy. M. bovis was isolated from the milk samples of all the 32 affected cows tested and from the mammary tissue of three affected cows that underwent necropsy. No other pathogens were isolated from these samples. Lesions in two of the necropsied cows were characterized by mild chronic suppurative mastitis and galactophoritis. The other necropsied cow showed a chronic necrosuppurative and pyogranulamaous galactophoritis, a condition not previously associated with M. bovis. M. bovis was detected immunohistochemically in the lumen of the affected mammary ducts suggesting that ascending infection via the teat canal was the likely route of transmission. No other intralesional pathogens were demonstrated microscopically.