太空搭载百子莲种子对其SP1代叶片形状的影响

本文对太空搭载百子莲（Agapaathus）DBH、BB、DB3个品种种子的SP1代样本单株的叶片长度、宽度和面积进行了统计分析,对实验数据进行了柯尔莫哥罗夫一斯米尔诺夫检验,计算了样本的列文统计量,并采用SPSS13．0统计分析软件检验了本假设的满足条件,进行了独立样本T检验。结果表明：DBH、BB的SP1代实验组和对照组叶片形状存在变异且叶长和叶宽均差异显著,DB差异不显著。     

航天诱变百子莲种子对其SP1代的影响

本实验研究了4个百子莲品种种子经航天诱变后其种子生命力和SPI代生长情况的变化。结果表明,航天诱变对百子莲种子的发芽率和出苗率有明显提高,除DB大花品种其发芽率与出苗率受空间条件抑制为0外;而对其SP1代生长情况的影响不很明显,其中BB的株高呈显著增高,DB早花的叶长、冠幅显著减小。     

Improvement bread characteristics of high level sunn pest (Eurygaster integriceps) damaged wheat by using transglutaminase and some additives

The purpose of this study was to improve the baking quality of high level sunn pest damaged wheat (HLSPDW; 20.6%) sample by using at varying levels transglutaminase, and fixed additive combination (diacetyl tartaric acid esters of mono and diglycerides + citric acid + L-ascorbic acid) with or without transglutaminase. It was observed that transglutaminase plays an important role in baking quality of HLSPDW. The increase in transglutaminase caused very clear increase on bread characteristics of wheat. Bread yield, height, pore structure, and crumb softness values increased sharply; weight loss and wideness of bread samples decreased accurately depending on increasing transglutaminase level. This increase did not affect obviously on bread quality at a certain proportion (0.3%). However, when the transglutaminase was more than 0.3% and depend on increase of percentage, bread characteristics showed significant increase. It was determined that, in the absence of transglutaminase; other additives could not improve the bread qualities examined. The unique application of using transglutaminase was found to be considerably to improve the bread quality of the HLSPDW. Overall results indicate that the properties of the bread from HLSPDW can be restored by the addition of transglutaminase. The highly disrupted protein structure present in the HLSPDW gluten requires higher transglutaminase concentrations.     

Ghrelin阳性细胞在食蟹猴消化系统中的分布定位

目的:研究ghrelin阳性细胞在食蟹猴消化系统中的分布定位。方法:采用免疫组化SABC法检测ghrelin免疫阳性细胞在食蟹猴消化系统中的分布部位、含量及细胞形态。结果:ghrelin免疫阳性细胞分布于食蟹猴的胃、十二指肠、空肠前段、空肠中段以及胰腺中,而在口腔黏膜、食管、空肠后端至直肠、肝脏中均无表达。结论:食蟹猴内ghrelin阳性细胞的分布与人和其他动物的分布基本相似,ghrelin在进化上具有保守性,物种间存在差异性。     

猪SEPW1基因的转录调控分析

本研究旨在对猪SEPW1基因的潜在启动子区进行克隆及转录活性分析，获得其核心启动子区域，并进一步分析转录因子SP1对SEPW1基因转录活性的影响，为探索SEPW1基因在猪肉质性状方面的功能奠定基础。利用实时荧光定量PCR检测SEPW1基因在大白猪各组织中的表达量，构建空间表达谱；通过PCR技术克隆得到6个逐级缺失的SEPW1基因启动子片段，构建6个双荧光素酶报告载体，通过检测各载体的双荧光素酶活性获得SEPW1基因的核心启动子区域；对核心启动子区进行生物信息学分析，发现潜在的SP1转录因子结合位点；通过过表达、抑制表达、定点突变及凝胶迁移试验（EMSA）确认SP1转录因子结合位点的存在及其对SEPW1基因转录活性的影响。结果显示，SEPW1基因在所检测的4月龄大白猪12个组织中均有表达，其中在腓肠肌及心脏中的表达量较高。双荧光素酶活性显示，猪SEPW1基因5'侧翼区-443~-231 bp为其核心启动子区，且-378~-306 bp存在1个潜在的SP1结合位点。过表达和抑制表达SP1基因结果显示，转录因子SP1能够促进SEPW1基因的转录；定点突变及EMSA试验确认，转录因子SP1可直接与SEPW1基因启动子区的SP1结合位点（-348~-339 bp）相结合。综合以上结果表明，转录因子SP1可直接靶向SEPW1基因的启动子区并促进SEPW1基因的转录。     

Photoshop软件在免疫组化图像分析中的应用

[目的]结合教学与科研工作实践,探讨采用图像处理软件Photoshop分析免疫组化图像的使用规范。[方法]选用合适方法对免疫组化图像进行分割与灰度转换处理,再采用软件测量与分析免疫组化图像,同时与Image-Pro Plus、ImageJ专业软件分析结果比较。[结果]该方法操作便捷,数据准确可靠。[结论]该方法值得在实验教学和科学研究中推广使用。     

雌激素受体α,β在雌性山羊肠系膜后神经节的表达

为探究雌激素是否通过交感神经调节雌性生殖系统的生理状态,试验选取雌山羊肠系膜后神经节,采用免疫组织化学SP法,检测雌激素受体α,β在肠系膜后神经节中的表达及分布。结果显示,雌激素受体α,β阳性产物在雌性山羊肠系膜后神经节广泛存在,强阳性产物存在于神经元胞膜和核中,阳性产物存在于整个神经元胞体以及核仁中。在神经节其他组成结构中也出现了阳性产物。表明在雌山羊肠系膜后神经元中存在雌激素受体,说明神经元对雌激素具有反应性;在神经节其他组成结构中出现阳性产物,说明雌激素可以经交感神经通路影响雌性生殖系统的生理机能,为研究雌性生殖系统的免疫神经调节提供了形态学证据。     

Distribution of ChAT in the Diencephalon of the One Day Chicken

The distribution of choline-acetyltransferase(ChAT)-like immunoreactive structure in the one day chicken diencephalons was examined by the method of streptavidin-Perosidase(SP)immunocytochemistry.It was found that ChAT immunoreactive neurons and fibers presented in many regions of one day chicken.Neural structure containing cholinergic neurons with varying density and immunoreactivity were the N.habexularis lateralis and N.habexularis medialis of the epithalamus,the N.rotundus,N.Ovoidalis,N.subrotundus and N.geniculatus lateralis of the thalamus,the hypothalamus,the N.decussationis supraopticae dorsalis of stratathalamus.There were also many varicose and nonvaricose fibers and terminals in the N.geniculatus lateralis and the hypothalamus.The results suggested that the diencephalons of one day chicken is richly innervated by the cholinergic neuron system.     

植酸酶产酶条件及热稳定性的研究

对黑曲霉９７０１、米曲霉９７１２ 、根霉９７０２ 三种霉菌进行固体培养法生产植酸酶的条件研究。结果表明：在３０ ℃±１℃条件下，黑曲霉９７０１ 培养７ｄ 产酶活力达１４０５２ｐｕ／ｇ；米曲霉９７１２ 培养６ｄ，产酶活力达１５３８７ｐｕ／ｇ；根霉９７０２ 培养６ｄ，产酶活力达１４０７０ｐｕ／ｇ。对黑曲霉９７０１ 用缓冲液（ｐＨ４８） 控制ｐＨ，在３０ ℃±１℃培养７ｄ，产酶水平达２１３１０ｐｕ／ｇ 。将黑曲霉９７０１ 植酸酶液在９０ ℃条件下保温１５ｍｉｎ，仍保留７６ ％ 的原酶活性。     

20%啶虫脒SL及其与三种农药混合防治杞柳瘿蚊田间药效试验

为有效控制杞柳瘿蚊为害,筛选出防治杞柳瘿蚊的理想药剂和最佳的施药剂量。试验采用茎叶喷雾法研究20%啶虫脒SL及其与3种化学农药混合对杞柳瘿蚊的防治效果。结果表明:20%啶虫脒SL单剂及其与3种农药混合对杞柳瘿蚊均有很好的防治效果,其中以每hm2用20%啶虫脒SL525ml对杞柳瘿蚊综合防治效果最好,其次是每hm2用20%啶虫脒SL375ml和每hm2用20%啶虫脒SL150ml+每hm2用40%毒死蜱EC750g。从生产成本、防治效果等因素综合考虑,推荐每hm2用20%啶虫脒SL375ml防治杞柳瘿蚊,或每hm2用20%啶虫脒SL150ml+40%毒死蜱EC750g混合防治杞柳瘿蚊。