Negligible contribution from roots to soil-borne phospholipid fatty acid fungal biomarkers 18:2ω6,9 and 18:1ω9

The phospholipid fatty acid biomarkers 18:1ω9, 18:2ω6,9 and 18:3ω3,6,9 are commonly used as fungal biomarkers in soils. They have, however, also been found to occur in plant tissues, such as roots. Thus, the use of these PLFAs as fungal biomarkers in sieved soil, which may still contain small remains of roots, has been questioned. We used data from a recent beech tree girdling experiment to calculate the contribution of roots to these biomarkers and were able to demonstrate that not more than 0.61% of 18:1ω9 and 18:2ω6,9 in sieved soil samples originated from roots (but 4% of 18:3ω3,6,9). Additionally, the abundance of the biomarker 18:2ω6,9 in the soil was found to be highly correlated to ectomycorrhizal root colonization, which further corroborates its fungal origin. PLFA biomarkers were substantially reduced in vital roots from girdled trees compared to roots of control trees (by up to 76%), indicating that the major part of PLFAs measured in roots may actually originate from ectomycorrhizal fungi growing inside the roots. We calculated, that even a near to 50% reduction in fine root biomass - as observed in the girdling treatment - accounted for only 0.8% of the measured decrease of 18:2ω6,9. Our results demonstrate that both 18:1ω9 and 18:2ω6,9 are suitable biomarkers for detecting fungal dynamics in soils and that especially 18:2ω6,9 is a reliable biomarker to study mycorrhizal dynamics in beech forests.     

冷温血停搏液心肌保护效应血清CK—MB,CTn—T的变化研究

为探讨血停搏液不同灌注对心肌保护效应，２８只低温体外循环犬，按停搏液灌注方式随机分为四组：Ａ组为冷晶组，Ｂ组为冷血组，Ｃ组为温血组，Ｄ组为冷温血组。     

家蚕幼虫氟中毒和质型多角体病毒感染后血淋巴中几种维生素含量的变化

运用高效液相色谱(HPLC)技术测定了家蚕五龄幼虫氟中毒和质型多角体病毒(CPV)感染后血淋巴中几种水溶性维生素含量的变化.为研究家蚕氟中毒和CPV感染的机理提供了依据.     

采用血液遗传标记对马亲仔关系案例的检测与分析

采用16个血液遗传住点对北京华骏育马场的275组及中国马术协会所提供的2组马匹进行了亲仔关系的鉴定，并对其中典型的8组马匹进行了分析。得出结果如下：6匹幼驹与其指定父母双方均不符而被排除亲仔关系，经验证确认为属于幼驹被串换；在所检的遗传住点中，以D血型、运铁蛋白(Tf)、蛋白酶抑制剂(Pi)和6-磷酸葡萄糖脱氢酶(6-PGD)检测亲仔关系错误的效果最好；在根据自身外貌无法断定为非纯血马的情况下，采用从非纯血马亲本来鉴定是行之有效的。     

家蚕血液酯酶缺失型的遗传学研究

家蚕血液酯酶电泳图谱中A区酯酶缺失型东34,是共显性等位血液酯酶A区酶带不表达基因BesAo的纯合子,基因符号Bes Ao/Ao。东34是其亲本交配后代的分离,选择,固定后的产物。东34缺失的A区酶带属胆碱酯酶类。     

牛早孕因子的分离纯化及其活性和含量测定

牛早孕因子的分离纯化及其活性和含量测定向际尧，李鹏程，孙文东，努尔波拉提（中国科学院新疆化学研究所生化研究室，乌鲁木齐８３００１１）早期妊娠因子（ＥａｒｌｙＰｒｅｇｎａｎｃｙＦａｃｔｏｒ，ＥＰＦ），由澳大利亚学者Ｈ。Ｍｏｒｔｏｎ等人在１９７４年应用玫...     

克伦特罗对绵羊肝脏血流量和氮代谢的影响

在4头门静脉、肝静脉、肠系膜静脉和股动脉上安装血管导管的绵羊中研究了克伦特罗（CL,0.8 mg/kgBW,肠系膜静脉给药,每天2次,连续5 d）对其肝脏生长代谢的影响。结果表明:CL对肝脏血流量影响较小。而在CL作用下绵羊血浆中尿素氮水平明显下降,肝静脉和门静脉血液尿素氮流量分别减少16.86％（P<0.01）和15.51％（P<0.05）。肝静脉多肽流量在CL处理期也较对照期下降38.71％（P<0.01）,门静脉处多肽水平处理期则与对照期相当。克伦特罗还增加绵羊肝脏IGF-I的合成和分泌,门静脉和肝静脉中的增加幅度分别为38.84％（P<0.01）和33.18％（P<0.01）。提示CL可通过增强对绵羊肝脏氮的储留及肝脏IGF-I的合成和分泌从而促进机体的生长代谢。     

岩羊血液生化遗传多态性的研究

采用聚丙烯酰胺凝胶电泳和火焰光度法对青海省19只岩羊13个血液生化遗传基因座的多态性特征进行了研究。结果发现：①ES2、ES3、ES4、S－LDH、S－ALP和TF6个基因座存在多态性，而KE、HB、AMY1、AMY2、AMY3、ES1、RBC－LDH和RBC－ALP7个基因座呈单态；②被检岩羊的多态性基因座比例为46．2％，表明岩羊的血液生化遗传基因座有颇大的遗传变异性。     

家蚕血液酯酶同功酶的研究

采用不连续聚丙烯酰胺凝胶电泳法.测定了24个家蚕品种及杂交种从三龄到后蛹不同发育时期的血液酯酶同功酶酶谱.结果表明:家蚕血液中存在着丰富的酯酶同功酶酶带类型,整个试验可观察到22条酶带,根据其迁移率及染色特点可分为A、B、C、D、E五大染色区.在不同发育阶段中共有13条酶带经常显现,后蛹期主要同功酶酶带活性降低,但非主带A-1区酶带却清楚地显示出来.在幼虫阶段,酶带的数目及活性防着食下量的增多而增人.并观察到A-2-b带与B-2带的表现最为突出,推测A-2-b带可能是家蚕血液部酶同功酶的基本酶带.进一步分析表明,A-2-b带活性与大多数经济性状间呈高度的正相关性.