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本实验用试管药物稀释了四种兽用抗生素对猪常见病原菌和正常菌的最小抑菌浓度 (minimal inhibitory concentration, MIC) 和最小杀菌浓度 (minimal bactericidal concentration, MBC)。通过实验发现:各种菌对四种药物的敏感性存在很大的差异,大多数细菌对药物C和D较敏感而对药物A和B存在较严重的耐药。 相似文献
83.
Veras HN Rodrigues FF Colares AV Menezes IR Coutinho HD Botelho MA Costa JG 《Fitoterapia》2012,83(3):508-512
Lippia sidoides Cham. (Verbenaceae) is used in the folk medicine as topical antiseptic in skin and mucous membranes and its therapeutic effect is attributed to the thymol presence. The objective of this work was to verify the chemical composition and antibiotic modifying activity of the essential oil extracted from the leaves of L. sidoides and its major component thymol. The essential oil was obtained by hydrodistillation and analyzed by GC/MS. The synergistic activity was evaluated using gaseous contact method. The essential oil was obtained (yield of 1.06%) and the GC/MS analysis identified the main constituents: thymol (84.9%) and p-cymene (5.33%). The antibiotic modifying activity was verified using the minimal inhibitory dose method and gaseous contact. It verified the interference of essential oil and thymol against all tested aminoglycosides. There were no statistical differences between the activity of the essential oil and thymol against Pseudomonas aeruginosa, indicating this to be the responsible composition for such activity. However, the oil was shown more effective when compared to the thymol against Staphylococcus aureus. The essential oil of L. sidoides and its major component thymol influence the activity of aminoglycosides and may be used as adjuvant in antibiotic therapy against respiratory tract bacterial pathogens. 相似文献
84.
拮抗放线菌BS-112的鉴定及抗菌物质粗提物对家蚕主要病原菌的抑制作用 总被引:2,自引:2,他引:0
以绿僵菌(Nomuraea rileyi)和黑胸败血病菌(Bacillus sp.)为靶标,从泰山林地土壤中分离获得一株对多种家蚕病原菌具有强烈拮抗作用的放线菌BS-112。根据该菌株的形态特征、培养特征、理化性质、细胞壁组分及16S rDNA序列分析结果,将其鉴定为吸水链霉菌(Streptomyces hygroscopicus)。该菌株抗菌谱广,抗菌物质耐高温,在酸、碱环境比较稳定,抗菌物质粗提物对黑胸败血病菌和绿僵菌的最小抑菌浓度分别为19.53μg/mL和78.13μg/mL,最小杀菌浓度分别为78.13μg/mL和156.25μg/mL,表明在较低浓度下即可达到杀菌效果。 相似文献
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本文观察中西药结合治疗猕猴奇异变形杆菌性腹泻的疗效。方法:将63例先后发病猴的诊治资料分为两组,后发病组41例,给予黄连素、藿香正气水等中成药,并配合敏感抗生素治疗;先发病组22例,当时只给抗生素治疗。结果:后发病组有效率85.4%,先发病组59.1%。两组疗效差异显著(P〈0.05)。结论:中西药结合治疗疗效优于单纯抗生素治疗。 相似文献
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In order to study the occurrence and co-infection of different species of Campylobacter, enteric Helicobacter and Anaerobiospirillum in dogs and cats and define a possible association between these microrganisms and gastrointestinal disorders, 190 dogs and 84 cats, either healthy or with diarrhea, were sampled between 2002 and 2003. Thirty-three C. upsaliensis, 17 C. jejuni, 2 C. helveticus, 1 C. lari isolates from dogs and 14 C. helveticus, 7 C. jejuni, 6 C. upsaliensis isolates from cats were identified using species-specific PCR and phenotypic tests. Whole cell protein profile analysis, phenotypic tests, PCR-RFLP of gyrB and a phylogenetic study of partial groEL and 16S rRNA sequences were used to identify 37 H. bilis, 22 H. canis and 14 H. cinaedi in dogs and 12 H. canis, 5 H. bilis and 2 H. cinaedi in cats. Whole cell protein profile analysis, phenotypic tests and species-specific PCR of 16S rRNA were used to identify 14 A. succiniciproducens, 12 A. thomasii isolates and one unidentified Anaerobiospirillum sp. isolate in dogs and 3 A. thomasii isolates in cats. Fifty-two animals (19%) were positive for the isolation of more than one genus. No significant statistical correlation was found between any isolates of Campylobacter, Helicobacter or Anaerobiospirillum spp. or the various co-infection rates, and the presence of diarrhea in either dogs or cats. Campylobacter isolates were also tested for antibiotic resistance using the agar dilution method. 相似文献