帽儿山林区主要树种树高与胸径之间的关系分析

为了进一步探索树高与胸径的相关关系,本研究以黑龙江省帽儿山林场为样地,选取林区中杨树（Polar）、白桦（Birch）、紫椴（Tilia）、枫桦（Betula costata）、水曲柳（Fraxinus mandshurica）林等5种主要树种为研究对象,通过SPSS17.0对胸径（D）和树高（H）的数据进行处理,应用7个常见树高-胸径模型来模拟相关关系,通过决定系数R2评价出了每个树种树高-胸径最优模型形式,所有树种最优模型的相关系数都达到了0.9以上,模型拟合的精度高.结论表明：①杨树的树高-胸径最优模型形式为幂函数曲线模型,方程式为：H=4.383D0.418;②白桦树、紫椴树、枫桦树的树高-胸径最优模型形式为三次曲线模型,方程式分别为：白桦树：H=4.785 ＋0.721D-0.008D2-0.00005D3;紫椴树：H =3.985＋0.787D-0.013D2-0.00009D3;枫桦树：H=6.345＋0.769D-0.010D2-0.000047D3;③水曲柳的树高-胸径最优模型形式为幂函数曲线模型,方程式为：H=e1.992＋0.043D.     

miR-17 regulates senescence of rat vascular smooth muscle cells

AIM: To investigate the effect of microRNA-17 (miR-17) on the senescence of vascular smooth muscle cells (VSMCs) and the underlying mechanism.METHODS: The medial layer of the thoracic aorta was collected from the SD rats and isolated for primary culture. VSMCs were identified by immunofluorescence staining. The VSMCs were collected at the 4th~6th generations, and then the miR-17 mimics and miR-17 inhibitor were transfected into the VSMCs by liposome method. After 24 h, the cell senescence was induced by D-galactose. The VSMCs were divided into the following 6 groups:aging induction+miR-17 mimics (A-miR-17) group, aging induction+miR-17 inhibitor (A-anti-miR-17) group, A-control group, normal (N)+miR-17-mimics (N-miR-17) group, N-anti-miR-17 group, and N-control group. On day 3 after the addition of D-galactose, the senescence of VSMCs was observed with β-galactosidase staining. The expression of miR-17, p16 and p21 was detected by RT-qPCR and immunohistochemistry. RESULTS: miR-17 expression in the VSMCs was significantly lower in A-control group than that in N-control group (P<0.01). Compared with A-control group, the expression of miR-17 in the VSMCs was significantly increased in A-miR-17 group (P<0.01), while that was significantly decreased in A-anti-miR-17 group (P<0.01). The number of β-galactosidase positive staining cells in A-anti-miR-17 group was significantly higher than that in A-miR-17 group (P<0.01). The expression of p21 at mRNA and protein levels in the VSMCs was significantly lower in A-miR-17 group than that in A-control group (P<0.01), and the expressions of p21 at mRNA and protein levels was significantly higher in A-anti-miR-17 group than that in A-miR-17 group (P<0.01). CONCLUSION: miR-17 inhibits rat VSMCs senescence induced by D-galactose, the underlying mechanism is associated with the inhibition of p21 expression.     

十七年“土改 合作化”小说中的中农

作为农村的中坚阶层，中农在相关政策层面一直被定义为中国革命的依靠和团结对象。但在部分“土改合作化”小说中中农的合法身份却不断遭受质疑。中农身份从理论到实践的不稳定性最终反映到文学中。这折射出土地改革的最终走向，并对当下中国社会持续产生各种影响。研究中农形象的特殊表现形态，对于反思“土改 合作化”叙事具有一定的文学史意义。     

高效液相色谱法测定鱼饲料中甲基睾丸酮含量的研究

试验建立了鱼饲料中17α-甲基睾丸酮的液相色谱检测方法。该方法以甲醇提取样品中的甲基睾酮,3β-甲基-17β-羟基-5-雄烯-7-酮为内标;梯度洗脱,流动相分别为水-乙腈溶液(80:20,V/V),流速0.5 ml/min;柱温40℃,DAD检测器检测,内标法定量,甲基睾酮的平均保留时间为11.29 min(R.S.D=0.20%),内标为8.68 min(R.S.D=0.19%)。结果表明,在7.5～60.0μg/ml范围内线性关系良好,拟合度为0.999 8,回收率在94%～100%之间,相对标准偏差为1.97%～3.21%。     

优质出口花生品种“花17”的应用研究

花生品种花17是一个传统出口品种。迁宁地区花生没有黄曲霉污染，但一直没有大连地区花生出口国外。引进并试种该品种，取得了良好效果，无论产量还是品质均受农民及外商好评，解决了农民“卖难”问题，又使农民收入增加，并为国家赚取了外汇。     

Controlled release of estradiol-17β and bisphenol A from a silicone tube for long-term administration in mice

Silicone tubes filled with estradiol‐17β (E2) or the xenestrogen, bisphenol A (BPA) were covered with a polyethylene tube and implanted into mice subcutaneously. The releasing patterns from the tube in vivo were obtained from the volume of estrogen remaining in the tube by measurement with high performance lipid chromatography. E2 in glycerol was released in a few days from a silicone tube, which was not covered with a polyethylene tube (Type A). However, E2 in sesame oil was released very slowly from the silicone tube covered with a polyethylene tube (Type B) and approximately 10% of E2 remained in the tube at 56 days after implantation into the mice. The mean linear regression equation for E2 was Y = 70.967 ? 1.2271X (R² = 0.9268) and the quadratic regression equation was Y = 77.97 ? 2.2773X + 0.0194 × 2 (R² = 0.9864). The remainder rate curves of E2 and BPA were not significantly different throughout the experimental period of 56 days. The Type B tube in the present experiment might be useful for long‐term administration of estrogens.     

子莲新品种建选17号的选育

以红花建莲、寸三莲65、太空莲2号为亲本，经杂交选育出子莲建选17号。该品种茎秆粗壮，生长势强，莲蓬扁圆形，蓬面直径11～16cm，结实率72％～85％，心皮平均25枚，莲子卵圆形，洁白，风味较好，通心白莲百粒干重约103g，一般每667m^2产干莲65kg，对腐败病和叶斑病的抗性强于红花建莲。