cDNA Cloning and Sequence Analysis of Rice Sbel and Sbe3 Genes

Two starch-branching enzyme (SBE) in rice, is known to be a key enzyme in amylopectin biosynthesis. The cDNA of two SBE(starch-branching enzyme) genes SheI and Shed encoding SBE Ⅰ and SBE Ⅲ (two major isoforms in rice) were cloned by an improved RT-PCR technique, from a template cDNA libray, derived from the total mRNAs extracted from the immature seeds of a japonica rice Wuyunjing 7. DNA sequence analysis showed that the size of the cloned SheI and Shed cDNAs were 2490 and 2481 bp long, respectively, including their entire coding sequences. Comparison analysis indicated that the nucleotide sequence of She3 was the same as that of shed (Genbank Accession No. D16201) as reported previously. There were only four base-pairs difference,which resulted in changes of two deduced amino acids between the cloned She1 cDNA and the reported she1 (Genbank Accession No. D11082). The cloned SheI and Shed cDNAs make it possible to improve rice starch quality through genetic engineering.     

氯霉素全抗原合成及多克隆抗体的制备

用重氮化方法将氯霉素与牛血清白蛋白（BSA）和卵清蛋白（OVA）偶联，制备免疫抗原和包被抗原，用ELISA方法进行鉴定。将偶联抗原用弗氏佐剂乳化后免疫德国大白兔。制备多克隆抗体，并用硫酸铵沉淀法初步纯化。用亲和层析法进一步纯化。用所得抗体建立竞争酶联免疫吸附分析法（ELISA）用于氯霉素检测，最低检查限为0.3ng/mL。     

全混合日粮在奶牛养殖业上的应用

全混合日粮(Total Mixed Rations,TMR)指的是根据奶牛不同泌乳阶段的营养需要,将粗饲料、精饲料、矿物质以及各种添加剂按照适当的比例进行充分混和成营养相对平衡的日粮进行饲喂的饲养技术.TMR饲养技术在配套技术措施和性能优良的TMR机械的基础上能够保证奶牛所采食的日粮都是精粗比例稳定、营养浓度一致的全价日粮.     

科技

中国分离首株甲型流感病毒并完成基因组序列测定，我国研制成功H5N1人禽流感活疫苗，     

装备展台

超宽型去皮机；绞肉机；鱼肉注水机；全自动化连续真空包装机；全自动连续式水煮机；上浆机[编者按]     

猪生殖与呼吸综合征病毒广东株ORF3和ORF5基因的克隆与分析

采用RT-PCR方法扩增了猪生殖与呼吸综合征病毒广东株的糖蛋白基因ORF3、ORF5，并对其进行了克隆和序列分析。用Blast分析软件比较分析了其与VR-2332株以及流行的欧洲株之间的同源性，并对其编码氨基酸序列的分子质量、等电点、穿膜区、糖基化位点进行了分析。结果显示，PRRSV广东株ORF3与美洲株的同源性达90％，而与欧洲株只是在318～393bp、502～697bp之间有77％左右的同源性；ORF5与美洲株的同源性为88％，与欧洲株仅在133～209bp区间有77％左右的同源性。     

全混合日粮在舍饲养羊中的应用分析

文章分析了我同当前养羊业的现状，大力发展肉羊生产是长远出路。TMR在肉羊生产上将比传统饲喂方式有较多的优势：生产方便，营养全面，便于机械化作业等，值得大力提倡和发展。     

绿色草原牧场大力发展全株青贮玉米生产

绿色草原牧场位于黑龙江省杜尔伯特蒙古族自治县。全场总面积57.2万亩,其中草原面积27.4万亩,占总面积的48％。近年来,牧场从实际出发充分发挥资源和地处世界养牛带及玉米种植带等优势,大力发展青贮生产,把以奶牛为主的畜牧业做为主攻方向来抓。采取龙头牵动、科技推动、利益驱动、政策带动等多项措施,