湿地松幼林施肥五年生长反应

在江西省永丰县恩江林场贫瘠的第四纪低丘红土立地进行湿地松林施肥试验，用以检验要对Ｎ、Ｐ、肥效的反应，和不同施肥期对幼林生长的影响，施肥后五年的生长统计分析表明：Ｎ、Ｐ配合施用效果最佳；施用量为Ｎ１００ｋｇ／ｈｍ＾２、Ｐ２Ｏ５１００ｋｇ／ｈｍ＾２时，立木蓄积达到４２．０１ｍ＾３／ｈｍ＾２，比不施肥增加９０％；单施肥湿地松幼林的生长反应也达到极显著水平。造林施Ｐ２Ｏ５５０－２００ｋｇ／ｈｍ＾２时，立木     

外来有害生物红火蚁风险分析及防控对策

参照国内外有害生物风险分析方法，通过对外来有害生物红火蚁在我国的分布状况、潜在危害性、危害对象经济重要性、定殖和扩散的可能性和危险性管理难度的定性和定量分析，得出风险评估值R=1．861，表明该虫在我国属于中度危险的有害生物。必须通过检疫、监测等措施加强对红火蚁的监管，防止其扩散、危害。     

花岗岩黄红壤杉木幼林施肥连年生长效应

１９９１￣１９９５年试验结果表明，在江西分宜花岗岩残积物发育的黄红壤上，杉木幼林施Ｎ、Ｐ、Ｋ及配合处理，使树高、胸径、蓄积总生长量指标分别达到５．６７￣６．５９ｍ、８．３５￣９．２８ｃｍ、６７．９２２￣９６．４１ｍ＾３／ｈｍ＾２，较对照增长５．６％￣２０．２％、７．４％￣１７．５％、２１．６％￣５６．２％。在各种施肥处理中，以５０ｋｇ／ｈｍ＾２Ｐ２Ｏ５的效果最佳，其连年生长量效应显著持续到第５年；     

利用人工授精技术改良半散放马鹿的研究

采用人工授精方法改良半散放马鹿 ,经过改良的马鹿 ,1日龄内主要体尺体重指标优于同期自然繁殖马鹿 ,其中体重增加 17.34% ,体斜长增长 12 .2 6 % ,肩高增加 11.2 7% ,且有较强的抗病能力。公鹿产茸量增加明显 ,初角茸增加 4 4.4 4% ,头锯茸增加 5 2 .0 7% ,2锯茸 6 1.39% ,按鹿茸的生长规律 ,到 5锯时鹿茸产量可提高 1倍     

Dormancy induction in container-grown Abies seedlings: Effects of environmental cues and seedling age

Twelve-week-old container-grown seedlings of noble fir (Abies procera Rehd.) and Shasta red fir (A. magnifica A. Murr. var. shastensis Lemm.), both high-elevation species, were grown under controlled environments in a study of induction of terminal-bud dormancy. Eight treatment combinations of long (15 h) or short (11 h) photoperiods, warm (25°/20°C) or cool (18°/12°C) thermoperiods, and dry (–1.2 MPa) or wet (–0.6 MPa) moisture regimes were imposed upon seedlings for 12 weeks. Treatment factors significantly affecting the induction of dormancy in terminal buds of seedlings were identified over time. The results suggest that seedlings responded dynamically to dormancy cues. The warm/dry and cool/wet combinations induced dormancy in the first 2 weeks in noble fir and in the first week in Shasta red fir. Short-day/warm and long-day/cool combinations enhanced dormancy induction in weeks 3 to 4 in noble fir and in weeks 1 to 7 in Shasta red fir. Short days and cool thermoperiods independently hastened dormancy induction in noble fir in weeks 5 through 12.     

Organic Matter Added to Bareroot Nursery Beds Influences Soil Properties and Morphology of Fraxinus pennsylvanica and Quercus rubra Seedlings

Bareroot hardwood seedling production involves intensive soil management. To increase soil organic matter (OM), nurseries commonly grow a cover crop for 1 year after every 1–2 year of seedling production. Raising soil OM levels can also be achieved through addition of soil amendments. We studied the influence of chicken manure (CM) and composted leaf, tree, and lawn trimmings (Cp) on soil properties and morphology of green ash (Fraxinus pennsylvanica Marsh.) and northern red oak (Quercus rubra L.) seedlings. CM was applied at 725, 1450, or 2900 kg ha⁻¹ (CM725, CM1450, and CM2900, respectively) and Cp was applied at 200 m³ ha⁻¹. Addition of CM and Cp significantly raised soil OM levels and altered soil chemical properties compared to the control (Ctrl). Root-collar diameter increased with addition of CM1450, CM2900, or Cp compared to CM725 or Ctrl plots for northern red oak, but was largest in soils amended with CM2900 for green ash. Conversely, height was greatest with addition of CM725 for northern red oak, but green ash seedlings were shorter in Ctrl plots than in all amendments except for CM725. Root volume of green ash and northern red oak seedlings was positively influenced by addition of CM or Cp. Seedling responses to nursery soil amendments vary with different forms and amounts of OM. Benefits to seedling growth through application of appropriate materials in the proper balance can improve seedling morphological quality and positively influence soil chemical properties.     

断母乳日龄对犊牛营养物质消化和血清生化指标的影响

本试验旨在研究不同断母乳日龄对犊牛营养物质消化和血清生化指标影响。试验选用新生红安格斯与西门塔尔杂交肉犊牛60头,根据日龄和体重随机分成5个处理,每个处理12头犊牛,公母各占1/2,试验组分别在28、42、56和70日龄进行断母乳(分别为28A、42B、56C、70D组),随后人工哺乳代乳品至90日龄;对照组( CK组)随母哺乳至150日龄。试验时间为出生至150日龄,期间测定体重(0、70、90、150日龄)和血清生化指标(70、90、150日龄)的变化。每个处理选取6头犊牛分别在断奶前(85~90日龄)和断奶后(145~150日龄)进行2期消化代谢试验。每期6 d,其中2 d预试期,4 d正试期。结果显示：1)试验全期体重各组间无显著差异(P>0.05)。2)断奶前各组犊牛营养物质消化率差异不显著(P>0.05),断奶后28A、42B组犊牛干物质消化率显著高于56C、70D组( P<0.05),有机物消化率显著高于70D组( P<0.05),其中42B组消化率略高。3)150日龄试验结束时,28A和42B组血清葡萄糖、总蛋白、甘油三酯、尿素氮、胆固醇、结合珠蛋白、白细胞介素-2、胰岛素样生长因子-1、免疫球蛋白M含量与CK组相比差异不显著( P>0.05),血清免疫球蛋白G、免疫球蛋白A含量分别显著低于、高于CK组( P<0.05),其中42B组略高。由此可见,红安格斯与西门塔尔杂交肉犊牛的断母乳时间以42日龄较为适宜。     

Phenotypic and genotypic characterization of canine pyoderma isolates of Staphylococcus pseudintermedius for biofilm formation

Biofilm-forming ability is increasingly being recognized as an important virulence factor in several Staphylococcus species. This study evaluated the biofilm-forming ability of sixty canine derived clinical isolates of S. pseudintermedius, using three phenotypic methods, microtiter plate test (MtP), Congo red agar method (CRA) and tube adherence test, and the presence and impact of biofilm-associated genes (icaA and icaD). The results showed that icaA and icaD genes were detected concomitantly in 55 (91.7%) of 60 isolates. A majority (88.3%) of the strains screened had matching results by the tube adherence test, MtP and PCR analysis. Better agreement (95%) was found between the PCR-based analysis and the CRA. Results of the icaA and icaD gene PCRs showed good agreement with CRA results, with a kappa of 0.7. Comparing the phenotypic methods, the statistical analysis showed that the agreement among the phenotypical tests using categorical data was generally good. Considering two classes (biofilm producer and biofilm non-producer), the percentage of matching results between the CRA method and the tube adherence test and between the CRA method and the MtP was 93.3%. A concordance of 100% was revealed between the MtP and the tube adherence test. The results indicate a high prevalence of the ica genes within S. pseudintermedius isolates, and their presence is associated with in vitro formation of a biofilm. A combination of phenotypic and genotypic tests is recommended for investigating biofilm formation in S. pseudintermedius.     

Investigation of biofilm production and icaA and icaD genes in Staphylococcus aureus isolated from heifers and cows with mastitis

Biofilm formation and antimicrobial resistance of Staphylococcus aureus are important virulence factors in cases of mastitis in dairy cows. However, few studies have investigated mastitis strains isolated from heifers. Within this context, the objective of the present study was to investigate biofilm formation on Congo red agar, the presence of the icaA and icaD genes by polymerase chain reaction (PCR), and the percentage of in vitro antimicrobial resistance of 110 S. aureus isolates from mammary gland secretions of heifers and cows with mastitis. PCR detected the icaA and icaD genes in 98% and 100% of isolates, respectively. However, only 55.5% of all isolates produced a biofilm on Congo red agar. Antimicrobial susceptibility testing revealed that 47.0% of isolates from heifers and 70.4% of isolates from cows were resistant to at least one of the antimicrobial agents tested. Resistance to penicillin and/or ampicillin was the most frequent (44.5%). These results indicate the need to implement prophylactic and control measures of mastitis for heifers. Heifers and cows can carry resistant strains with the capacity of biofilm production, a fact representing a threat to public health and animal well‐being and generating losses to dairy farmers.     

外源多酚添加条件下干红葡萄酒颜色和花色苷特性研究

颜色是红葡萄酒重要的感官特征和质量评价指标,花色苷是红葡萄酒呈色、稳定和营养功能的重要物质基础。既往研究极少探讨外源添加天然色素对红葡萄酒颜色质量和花色苷的影响。选取赤霞珠干红葡萄酒为研究对象,于酒精发酵前和发酵后分别添加天然色素Ⅰ和天然色素Ⅱ2种色素,并以添加黄岑苷、绿原酸和没食子酸3种多酚为对照,探究对葡萄酒颜色特征、花色苷种类与含量的影响。结果发现：在发酵前添加天然色素Ⅰ和Ⅱ使得酒体颜色加深、红色色调加强、花色苷含量增加。在发酵后添加以上多酚对葡萄酒颜色和花色苷的影响弱于发酵前添加,但添加天然色素Ⅰ仍然有利于花色苷含量的保持,只是效果弱于发酵前添加。其余多酚物质处理对供试酒样颜色品质影响较小。研究结果表明发酵前添加多酚物质更有利于红葡萄酒颜色品质的提升和稳定、花色苷含量的保持和稳定,天然色素Ⅰ是相对最优质的辅色剂。