三级串联人工快渗系统处理高氨氮生活污水运行参数的优化

[目的]进一步优化三级串联人工快渗系统处理高氨氮生活污水的运行参数。[方法]以校园学生生活区高氨氮生活污水为研究对象,从水力负荷、湿干比以及水力负荷周期等方面,通过模拟柱试验确定其最佳运行参数。[结果]最佳运行参数为水力负荷1.0m/d,湿干比为1∶5,水力负荷周期为4 h,出水COD、NH4-N的浓度均能满足《城镇污水处理厂污染物综合排放标准》（GB18918-2002）一级A标准。碳源不足是影响三级串联人工快渗系统脱氮效率较低的主要限制因子。缩短水力负荷周期,可以有效增加系统的复氧效率,提高系统对COD、NH4-N的好氧生物降解效率。[结论]该研究为三级串联人工快渗系统的实际工程应用奠定了理论基础。     

迷迭香组培快繁技术研究

以迷迭香茎段为外植体进行组织培养,结果表明:在M S 6 BA 4.0m g/L NAA 0.1m g/L培养基上培养一周后,诱导出幼芽;M S 6 BA 3.0m g/L KT 0.2m g/L NAA 0.1m g/L培养基对丛生芽的增殖效果最好,增殖倍数达3.70;生根培养基选用1/2M S NAA 0.5m g/L为最佳,生根率达86.7%。     

不同植物生长调节物质对天目地黄组织培养及植株再生的影响

以天目地黄幼叶片为外植体,研究不同植物生长调节物质对其组织培养和植株再生的影响.结果表明,实验条件下,诱导叶片分化形成愈伤组织的最佳培养基为MS 6-BA1.0 mg.L-1 2,4-D 0.2 mg.L-1;最适的丛生芽分化培养基为1/2 MS 6-BA1.0 mg.L-1 IBA0.05 mg.L-1;诱导丛生芽生根的最佳培养基为1/2 MS IBA0.1 mg.L-1.并在此基础上探讨了组织培养技术在地黄属植物药用及园林方面的开发和利用前景.     

Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: metabolite and mineral profiles in blood and milk

The objective of this study was to determine the effect of evaporative cooling and dietary supplemental Zn source on blood metabolites, insulin and mineral concentrations, and milk mineral concentrations following intramammary lipopolysaccharide (LPS) infusion. Seventy-two multiparous Holstein cows were assigned to one of four treatments with a 2 × 2 factorial arrangement. Treatments included two environments: with or without evaporative cooling using fans and misters over the freestall and feedbunk, and two dietary sources of supplemental Zn: 75 mg/kg of dry matter (DM) supplied by Zn hydroxychloride (inorganic Zn; IOZ) or Zn hydroxychloride (35 mg of Zn/kg of DM) + Zn–Met complex (ZMC; 40 mg of Zn/kg of DM). A subset of cows (n = 16; 263 ± 63 d in milk) was infused with 10 μg of LPS or a saline control in the left or right rear quarters on day 34 of the environmental treatment. Individual milk samples collected from LPS-infused quarters at −4, 0, 6, 12, 24, 48, 72, 96, and 144 h relative to infusion were analyzed for minerals. Blood samples were collected at the same time with an additional sample collected at 3 h post-infusion to analyze glucose, nonesterified fatty acids (NEFA), insulin, and minerals. Cooling by time interactions (P ≤ 0.07) were observed for plasma glucose, NEFA, and serum insulin. Compared with cooled cows, non-cooled cows had lower concentrations of plasma glucose except at 3 h following intramammary LPS infusion, greater serum insulin at 3 and 12 h, and lower plasma NEFA at 24 and 48 h after infusion. Relative to cooled cows, non-cooled cows tended (P = 0.07) to have lower serum K concentration and had lower (P < 0.01) serum Zn 6 h following infusion (cooling by time interaction: P < 0.01). Relative to ZMC cows, IOZ cows had greater (P ≤ 0.09) concentrations of plasma Se, skim milk Na and Se, and skim milk Na to K ratio. Regardless of treatment, intramammary LPS infusion reduced (P < 0.01) serum or plasma concentrations of Ca, Mg, Zn, Fe, and Se, but increased (P < 0.01) their concentration in skim milk. In conclusion, deprivation of cooling resulted in more rapid and prolonged insulin release and influenced the systemic and mammary mineral metabolism during mammary inflammation induced by LPS of lactating dairy cows. Dietary supplementation of Zn–Met complex reduced blood and milk Se concentrations compared with cows fed Zn from an inorganic source.     

5W吉福特-麦克马洪制冷机及实验测量

本文介绍了信息产业部电子十六所独立开发研制的5W／20K闭式循环G－M制冷机,该机型既可作为低温泵的冷源,也可用于低温电子器件和超导器件的冷却。该机主要性能指标为;制冷量＞5W／20K;降温时间＜45min;重量＜12kg。     

ED-XRF法快速测定虾预混合饲料中钙、磷含量

虾预混合饲料中钙（Ca）、磷（P）含量直接影响虾的生长、发育和健康状况。传统的化学分析方法需要对虾预混合饲料进行复杂的预处理,取得测试结果需要花费数小时。采用一种简单、快捷的分析方法——能量色散X射线荧光光谱法（ED-XRF）,对虾预混合饲料进行粉碎、压片和测试,在数分钟内获得了样品的测试结果。ED-XRF法对Ca、P的检出限分别为43 mg/kg和163 mg/kg,定量限分别为142 mg/kg和544 mg/kg。对虾预混合饲料3^#样本连续测试6次,Ca、P的连续测定数据的极差分别为0.159%和0.013%,分别小于推荐性国家标准中Ca、P含量检测的重复性临界差CrR₉₅（6）0.239%和0.031%,表明ED-XRF法满足推荐性国家标准对饲料中Ca、P含量测定的要求。通过对10个已定值的虾预混合饲料样品进行Ca、P含量测定,ED-XRF法的测定值与推荐性国家标准中相关方法测定值的相对差值不大于4.55%。综上表明,ED-XRF法具有较高的精密度和准确度,可用于虾预混合饲料生产企业对Ca、P元素添加量的监控。     

矮牵牛快速繁殖的研究

以矮牵牛(Petunia hyvrida Vilm)叶片作为外植体。实验表明矮牵牛快速繁殖诱导分化培养基为Ms+6-BA1.0mg/L+NAA0.5mg/L。生根培养基为1/2MS+IBA0.1mg/L。试管苗移栽最合适的基质是珍珠岩。     

犬细小病毒实时荧光RPA检测方法的建立

由犬细小病毒(CPV)引起的犬细小病毒病是我国出入境口岸宠物检疫的重要疫病。为了提高检疫效率,本研究拟建立检测CPV的实时荧光重组酶聚合酶扩增(RPA)方法并进行评价。本研究根据CPV衣壳蛋白基因序列设计RPA引物,以GB/T 27533-2011中的PCR方法作为参照,通过检测其他4种犬病毒和3种其他种属的细小病毒来分析实时荧光RPA的特异性、检测不同浓度的模板分析实时荧光RPA的灵敏度、检测CPV阳性或阴性犬的不同组织、血液、尿、粪便等样本以及7株CPV野毒株来分析实时荧光RPA的稳定性。结果显示,本研究建立的实时荧光RPA特异性好、灵敏度高,检测临床组织、体液、粪便样本以及野毒株时都具有良好的稳定性。结果表明,本研究建立的实时荧光RPA可满足CPV快速检测要求。     

猪笼草组培快繁技术研究

以猪笼草的顶芽和腋芽为外植体进行组培快繁研究,结果表明:在1/2MS 6-BA 1mg/L NAA 0.1mg/L培养基中培养10d后,能诱导出幼芽;1/2MS 6-BA 2mg/L NAA 0.1mg/L培养基对丛生芽的增殖效果最好,增殖倍数达4.1;生根培养基以1/4MS 6-BA 0.1mg/L NAA 0.5mg/L为最佳,生根率可达95%以上。