茶道哲学的工夫境界与生命体验

工夫境界是研究中国哲学的核心内容之一。以工夫境界解读中国茶道哲学,有助于进一步理解中国茶道哲学的工夫与境界。中国茶道哲学的核心在"和"。茶人通过一系列的工夫持守,力求进入到"和"境界。在"和"的工夫境界观照下,茶人的生命体验与心灵体验高度凝结,进以凸显中国哲学与文化中独有的"天人合一"思想,使中国茶道哲学思想提升了又一高度。     

Metal leaching and toxicity of denitrifying woodchip bioreactor outflow—Potential reuse application

The denitrifying woodchip bioreactor is designed to remediate nitrate-rich water, including those produced from aquaculture effluents. Reuse of treated bioreactor outflows in recirculating aquaculture would offer considerable water savings and valuable alkalinity recuperation. However, such bioreactors may leach detrimental wood-bound contaminants, preventing outflow reuse. To determine water reuse potential, woodchip media from two hardwood species (white ash, Fraxinus americana; Norway maple, Acer platanoides) were evaluated for 206 d under a range of operating conditions (start-up, steady-state, reducing conditions, and drying-rewetting cycles) for a spectrum of potentially harmful dissolved contaminants. Aerated outflows also were evaluated for acute and chronic toxicity to the biologically sensitive invertebrate Ceriodaphnia dubia. Dissolved metal leaching subsided within the first few weeks of operation, though initial concentrations of copper and zinc were detected at concentrations of concern. Elevated concentrations of tannins-lignin and total ammonia nitrogen were detected throughout the study and were influenced by operational phase. Acute toxicity was not generally detected, though chronic toxicity was observed during drying-rewetting cycles in the maple outflows. The measured toxicity was not correlated with water chemistry, indicating an additive effect of several toxicants. Overall, significant differences in outflow water quality between ash and maple wood species were negligible. Results indicated that bioreactor outflows may be applicable for aquacultural reuse, though reusing outflows immediately following start-up or restarting after a dry period would not be recommended.     

果蔬型甜玉米新品种京科甜191的选育

京科甜191是以超甜玉米自交系T68为母本,自交系T6302为父本,通过杂交选育而成的果蔬型甜玉米品种。果穗筒型,穗长19.0 cm,穗粗5.3 cm,穗行数16~18行,行粒数36~38粒,粒行整齐。籽粒亮黄色,外观漂亮。平均每667 m2鲜果穗产量900 kg左右,出籽率约67.4%。鲜籽粒含粗蛋白2.98%,粗脂肪1.37%,总糖6.7%,口感甜脆清爽,皮薄无渣。播种至采收平均85.4 d(天),适宜京津冀及类似生态区种植。     

马疱疹病毒1型主要毒力基因分析及TK基因缺失载体的构建

为了解新疆马疱疹病毒1型（EHV-1）主要毒力基因遗传进化情况并构建TK基因缺失株，本研究以EHV-1 XJ2015株DNA为模板，对其主要毒力基因TK、gI和gE全长进行克隆、测序及生物信息学分析，并扩增TK基因左右重组臂TKL和TKR，构建质粒pUC-TKLR，将扩增后的增强绿色荧光蛋白（EGFP，含有CMV+polyA）插入pUC-TKLR质粒，构建TK基因缺失打靶质粒。TK、gI和gE基因同源性分析结果显示，XJ2015株与国外EHV-1分离株TK、gI和gE基因同源性均较高，分别为99.8%~100.0%、99.6%~100.0%和99.9%~100.0%；与EHV-3分离株同源性均最低，分别为72.9%、59.4%和62.1%；遗传进化分析显示，3个基因均与国外EHV-1同属于一个遗传进化分支，与EHV-9和EHV-4进化关系较近，但与EHV-3进化关系较远，表明XJ2015毒株与国外EHV-1毒株TK、gI、gE基因核苷酸上差异不明显，没有明显的地域性特征，功能基因保守且进化缓慢，同源基因功能相同或相近；经PCR扩增、酶切、测序及转染鉴定，本试验成功构建了用于TK基因缺失的打靶质粒pUC-TKLR-EGFP。通过对EHV-1主要毒力基因的分析及TK基因缺失打靶载体的构建，为新疆地区马鼻肺炎流行病学调查分析、TK基因缺失株的构建提供理论依据。     

Reproduction in three species of rainbowfish (Melanotaeniidae) from rainforest streams in northern Queensland, Australia

Abstract – The reproductive biology of three species of rainbowfish (Melanotaeniidae) in northeastern Australian rainforest streams was investigated. Two species, Melanotaenia eachamensis and Cairnsichthys rhombosomoides are endemic to the area, whereas the third, M. splendida splendida, is more widespread. The species were all highly fecund, producing many hundreds of eggs between 1.10 and 1.24 mm in diameter. Melanotaenia eachamensis was the most fecund, produced the largest eggs of the three species, and consequently exhibited the greatest maternal investment (as measured by gonadosomatic index). The majority of reproductive effort occurred during the dry season, although reproductively active fish were present year-round for each of the species, but particularly so for M. s. splendida and C. rhombosomoides. No evidence for a role by temperature or photoperiod as environmental cues for reproduction was found, and it was suggested that gonad development was strongly tied to somatic growth. The concentration of reproduction to the dry season ensures that larvae are produced during a period of relatively stable and benign physical conditions. Comparison of temporal changes in gonadosomatic index values suggest that the spawning season of M. eachamensis , which occurs in high-elevation streams, is more restricted and commences about 1 month earlier than either other species. A similar phenology was observed for the M. s. splendida population found at high elevation and highlights the potential for spatial differences in stream productivity to influence life history. ^Note     

猪口蹄疫O型灭活疫苗PD50效检攻毒方式探索

用10 000 TCID50的OR/80株F13细胞毒或用10 000 LD50的OR/80株乳鼠组织毒(ORMF8),通过肌肉注射途径、蹄踵球部或趾间皮内注射途径接种O型口蹄疫抗体阴性架子猪进行攻毒试验,三种途径均不能使试验猪产生规律性发病.     

南方山区草地资源的类型及开发利用途径

根据生态学的基本原理，对我国南方山区草地资源的特点进行了分析，从生态学类型上对该地区草地进行了划分，进而提出了南方山区草地开发利用的途径。     

蛋鸡血清硷性磷酸酶变化规律的研究

本试验以京白Ⅲ系为试验材料,测定了四个时期的血清AL P活性,研究了其变化规律。结果表明,AL P同工酶型快型酶活性显著高于慢型。年龄、同工酶型及年龄×同工酶型互作对AL P活性有显著影响。AL P活性与产蛋量间遗传相关表现为,在生长期为正,而产蛋期为负。四个年龄中,AL P做为间接选择指标,以6周龄为最佳.     

梨三倍体新品种龙园洋红的选育

龙园洋红梨是梨三倍体新品种,亲本为56-520×乔玛,抗寒性强,短枝型.果实不规则长椭圆形,果形指数1.13,平均单果重185.5 g,每个果实平均2.5粒种子,种子小、不饱满,多畸形.果皮浅黄色,阳面有红晕,果肉乳白色,肉质细软多汁,风味甜,有香气,可溶性固形物16.05%.果实在哈尔滨地区9月中旬成熟.