Acute and Sub-chronic Toxicity Test on Rats of a New Kind of Antidiarrheal Chinese Herbal Medicine Compound Preparation for Livestock

In order to understand the security of a new kind of antidiarrheal Chinese herbal medicine compound preparation for livestock,acute and sub-chronic toxicity test were conducted.Acute toxicity test used the largest drug dose method,20 Wistar rats were orally treated with the Chinese medicine compound preparation.In the sub-chronic toxicity test,80 rats were randomly divided into 4 groups with 20 rats in each group and orally given a dose of 3 000,1 500,750 and 0 mg/(kg·BW）of Chinese medicine compound preparation once a day for 30 days.The general clinical status was observed,rats weight were measured and the dose was adjusted every week during the test,after the test measured blood routine index,biochemistry index,and preceded the gross anatomy observation,weighing each major organs and calculated the viscera coefficient,and proceded main viscera histopathological observation between the high dose group and the control group.The acute toxicity results showed that every rat would be alive gavaged with the lethal dose（LD₅₀）of compound preparation larger 5 g/(kg·BW).The sub-chronic toxicity autopsy showed that except heart,lung,and testicles in individual rats appeared mild bleeding in the high dose group,the other dose group organs found no abnormal change.The haematological index showed except mononuclear cell rate（P<0.05),and hematocrit declined significantly（P<0.05）in the high dose group,all the indexes of the other groups were in the normal range,there was no significant difference from the control group.The test suggested the Chinese medicine compound preparation was no toxicity under the condition of this test according to acute toxicity classification standard of exogenous chemicals by WTO,there was no effect on the growth and development of rats in the sub-chronic toxicity test,and there was no chronic toxicity at least 1 500 mg/kg feeding conditions in short-term repeated application.     

Vachellia karroo leaf meal: a promising non-conventional feed resource for improving goat production in low-input farming systems of Southern Africa

Among a vast number of forage trees, Vachellia karroo (Acacia karroo) remains one of the most widely distributed indigenous plant legumes in Southern Africa. The plant possesses some striking attributes, such as rapid growth rate, drought tolerance, adaptation to alkaline and acidic sterile soils, and resistance to a variety of edaphic and temperature changes. In past years V. karroo was recognised to be a major threat to rangeland productivity owing to its invasive nature. Research interest has shifted from its eradication as an unwanted plant to its utilisation as a feed resource for livestock. Extensive utilisation of V. karroo is mainly hindered by the presence of tannins and spines. Prospects to lessen the adverse effects of the anti-nutritional influences are now recognised. Vachellia karroo possesses desirable fatty acid profiles, and high protein and mineral contents that can improve animal performance. Presently, the use of V. karroo for goat production in communal areas is restricted owing to limited evidence for its nutritional value as animal feed. The present review, therefore, seeks to demonstrate the utility potential of V. karroo to improve growth performance, carcass characteristics, chevon quality, meat fatty acid profiles and as a natural anti-helminth for goats raised under an extensive system of production.     

Formulation challenges of organic poultry diets with readily available ingredients and limited synthetic methionine

Organic agriculture has increased greatly over the past several years as consumer demand for these products has risen. Organic meat production, including poultry, has grown especially quickly, resulting in increasingly stringent guidelines being put in place to ensure safe, uniform, and ideal products for these consumers. Feed formulation and manufacturing for organic poultry in the United States are strictly regulated by the United States Department of Agriculture National Organic Program. Currently, no synthetic amino acids are allowed to be used in organic poultry diets in the United States except for limited quantities of synthetic methionine since it is typically considered the first or second limiting amino acid in corn and soybean meal based poultry diets and it is a critical nutrient for maintaining bird growth and productive performance, especially regarding egg size in laying hens. The aim of the review herein is to discuss the impacts that current organic regulations on synthetic methionine have on diet formulation when using readily available ingredients. High levels of crude protein must be used to meet bird methionine requirements with this restriction in place, which both increases feed costs and can have negative impacts on bird health and on air quality and the surrounding environment due to increased ammonia emissions. Atypical feed formulation strategies have been explored to address this issue, including such methods as feeding alternative ingredients and providing forage materials. Another possibility would be to use an average lifetime maximum inclusion rate for synthetic methionine, which may allow for better flexibility to provide sufficient methionine throughout various phases of growth and production since the requirements of the bird change with age.     

Deoxynivalenol in chicken feed alters the vaccinal immune response and clinical biochemical serum parameters but not the intestinal and carcass characteristics

This study was conducted to investigate the impacts of deoxynivalenol (DON) feeding either alone or in combination with a microbial feed additive (MFA) on the immune response to a viral vaccine and serum clinical chemical parameters. Forty 1‐day‐old boiler chicks were weighed and randomly divided into four groups, 10 birds in each group: (i) control group fed with basal diet; (ii) DON group fed with basal diet artificially contaminated with 10 mg DON/kg feed; (iii) DON + MFA group fed with basal diet contaminated with 10 mg DON/kg feed and supplemented with 2.5 kg of MFA/ton feed; and (iv) MFA group fed with basal diet supplemented with 2.5 kg of MFA/ton feed. At 35 days of age, birds were slaughtered and blood was collected for investigating the antibody titre against infectious bronchitis virus (IBV) and clinical chemical parameters. The results showed that DON reduced (p = 0.032) the titre against IBV, decreased (p = 0.005) the level of alanine transaminase (ALT) (4.2 ± 0.5 U/l) compared with control birds (6.4 ± 0.5 U/l), increased (p = 0.002) the serum cholesterol concentration (144 ± 6 mg/dl) compared with their control counterparts (123 ± 5 mg/dl) and increased (p = 0.074) the amount of circulating triglycerides (62.25 ± 7.50 mg/dl) compared with controls (39.55 ± 4.74). These results indicate that dietary DON altered the humoral immune response to viral vaccine and affected the serum clinical biochemistry. However, DON in combination with MFA did not affect serum IBV titre. Taken together, DON in the feed of broilers produced an impairment of the success of IBV vaccine and affected the health of birds.     

Effect of dietary probiotic,prebiotic and synbiotic supplementation on performance,immune responses,intestinal morphology and bacterial populations in broilers

This study was conducted to investigate the effects of probiotic (Primalac), prebiotic (TechnoMos) and synbiotic (Primalac + TechnoMos) supplementation on performance, immune responses, intestinal morphology and bacterial populations of ileum in broilers. A total of 240 one‐day‐old broiler chicks were randomly divided into four treatment groups which included 60 birds. Control group did not receive any treatment. The chicks in the second, third and fourth groups were fed probiotic (0.9 g/kg), prebiotic (0.9 g/kg) and probiotic (0.9 g/kg) plus probiotic (0.9 g/kg; synbiotic), respectively, at entire period. Daily feed intake, daily weight gain and feed conversion ratio were evaluated. The birds were immunized by sheep red blood cell (SRBC) on days 12 and 29 of age and serum antibody titres were measured on days 28, 35 and 42. Newcastle vaccines administered on days 9, 18 and 27 to chicks and blood samples were collected on day 42. Intestinal morphometric assessment and enumeration of intestinal bacterial populations were performed on day 42. The results indicated that consumption of probiotic, prebiotic and synbiotic had no significant effect on daily feed intake, daily body weight gain, feed conversion ratio, carcass traits, intestinal morphology and bacterial populations of ileum (p > 0.05). Consumption of prebiotic increased total and IgM anti‐SRBC titres on days 28 and 42 and antibody titre against Newcastle virus disease on day 42 (p < 0.05). Synbiotic increased only total anti‐SRBC on day 28 (p < 0.05). It is concluded that consumption of prebiotic increased humoral immunity in broilers. Therefore, supplementation of diet with prebiotic for improvement of humoral immune responses is superior to synbiotic supplementation.     

早期限饲对肉鸡肌肉生长及生产性能的长期影响

本研究旨在比较不同生长阶段隔日限饲(早期限饲:1~14日龄限饲,后期限饲:50~63日龄限饲)对肉鸡肌肉生长及相关激素水平的影响,并探讨早期限饲对肉鸡肌肉生长的程序化作用。试验结果:1)早期限饲组整个试验阶段体重均极显著低于对照组,后期限饲组限饲一周后体重显著低于对照组并于63日龄实现完全补偿生长;2)早期限饲组14日龄和63日龄胸肌、腓肠肌重均显著低于对照组;后期限饲组63日龄胸肌、腓肠肌重均显著低于对照组,且二者与早期限饲组均差异不显著;3)早期限饲显著降低了14日龄肉鸡肌肉中DNA、RNA和蛋白含量,63日龄恢复至正常水平,且核酸、蛋白水平在胸肌和腓肠肌内的趋势表现不一致;4)早期限饲组14日龄血清中T3、T4水平均显著低于对照组,63日龄血清T3水平仍显著低于对照组;后期限饲组63日龄血清T3水平显著低于对照组,T4水平与对照组差异不显著。结果表明:1)限饲可显著阻碍肉鸡肌肉的生长;2)不同部位的肌肉对限饲的敏感度不同;3)早期营养因子对肉鸡肌肉的生长具有"程序化"作用,甲状腺激素可能参与这些过程的调节。     

Effects of Compound Plant Nutrients on Rumen Fermentation Parameters,Rumen Microbiota and Fatty Acid Composition of Longissimus dorsi Muscle in Finishing Small Tail Han Sheep北大核心CSCD

This study was conducted to investigate the effects of compound p1ant nutrients (CPN) on rumen fermentation parameters, rumen microbiota and fatty acid composition of longissimus dorsi musc1e in finishing sma11 tai1 Han sheep. Sixteen 4-month-o1d finishing sma11 tai1 Han sheep with an initia1 body weight (BW) of (24.18±0.31) kg were random1y divided into two groups, name1y, with 8 rep1icates per group and 1 sheep per rep1icate. The sheep in the contro1 group were fed a basa1 diet, whereas the sheep of the contro1 group (CPN group) was fed the basa1 diet supp1ementation with 3‰ CPN. The experiment 1asted for 97 days after 7 days adaption. The resu1ts showed as fo11ows: compared with the contro1 group, 1) adding CPN decreased the concentration of ammonia nitrogen (NH3-N) (P<0.05); 2) CPN supp1ementation affected beta diversity of rumen microbiota; 3) the re1ative abundance of Acidobacteriota, Erysipe1otrichaceae_UCG-002, Lactobacillus, and Megasphaera were enhanced by adding CPN (P<0.05), whereas, the re1ative abundance of Bacteroidetes and Prevotella was 1ower (P<0.05); 4) the supp1ementation of CPN had no significant effect on fatty acid composition of longissimus dorsi musc1e of fattening sma11-tai1ed Han sheep (P>0.05); 5) the contents of tota1 MUFA, C18:1n9c, C14:1, C16:1, C18:2n6c, C18:3n3 and n-3PUFA in the longissimus dorsi musc1e were corre1ated with the re1ative abundance of Megasphaera, Erysipe1otrichaceae_UCG-002, Succiniclasticum and Ruminococcus (P<0.05). In conc1usion, CPN can regu1ate the rumen microbiota structure and reduce the rumen NH3-N concentration of fattening sma11-tai1ed Han sheep. In production practice, CPN can be used as a rumen eco1ogica1 regu1ator. © 2023 Authors. All rights reserved.     

Analysis of Radial Forces on the Seal Block in a Compound Gear Pump with 3 Idle Gears

By analyzing the pressure distribution between gears and sealing blocks in compound gear pump with three idlers,the resultant hydraulic forces acting on seal blocks are obtained.The conclusion is the unbalanced resultant hydraulic forces are relatively small in magnitude.In the pump,the inner sealing edges are long and so it s Sealing effect is good,but the outer sealing effect is relatively poor because of its short sealing edges.The little unbalanced force pushes the sealing block to te pumping chamber,therefore it is advantageous to meliorating the pump's sealing effect,thereby to improving it's volumetric efficiency as well.     

Experimental Study of Multifunctional Acid to Deliquesce Stratum-jam

Aiming at the oneness of acid to deliquesce stratum-jam applied in scene at the present time,which is incapable of deliquescing complex dirty simultaneously,this article confects a new multifunctional O/W acid.This acid is an emulsified mixture containing 15% hydrochloric acid as its extro-posture,high dissolved oil as its internal state under the function of some proper exterior active reagent.It is composed of 0.5%OP-10,0.5%Span80,0.2%Tween80,0.06%imidazole and 0.01%twelve-alcohol,and the content of oil is equal to the acid content.It can be indicated that this acid has good ability of resolving mineral(CaCO_3,MgCO_3) and organic compound(mainly colloidal bitumen) when it was poured into the stratum,so that it can deliquesce oleic stratum-jam,resume and improve the penetrability of stratum,boost the ability of liquid flowing up.     

Experimental Study of Compound Rock''s Behavior at Rock Salt''s Over-layer

Based on the experimental study of the compound rock's behavior at rock salt's over-layer under single compression and tri-axial compression condition, the basic mechanical properties of the rock have been obtained. It could serve as reference to research work and production.