负压供水下盆栽大豆叶片的光合生理研究

试验采用一种自制装置,在负压灌溉原理的基础上,通过设置一系列供水吸力梯度,结合盆栽方式对大豆叶片叶绿素含量、光合及荧光参数的变化进行了研究.结果表明:供水吸力增加,土壤重量含水量下降,大豆叶片叶绿素a(Chl a)、叶绿素b(Chl b)含量降低,类胡萝卜素(Car)含量增加; 吸力处理在50～70 hPa时光合参数均保持在较高的水平,气孔限制是光合速率(P n)下降的主要原因,120、140 hPa处理时非气孔限制起主要作用;随着光强(PAR)的增加,电子传递速率(ETR)增加,实际光量子产量(Yield)与光化学淬灭(qP)下降,非光化学淬灭(qN)上升,4个处理中50 hPa吸力下的大豆叶片活性较高.     

水、氮效应与叶绿素关系试验研究

以冬小麦和夏玉米为试验对象，研究了不同水、氮条件对作物植株含氮及叶绿素含量的影响，作物叶片含氮、作物产量与叶绿素含量之间的量化关系，并提出了利用叶绿素仪预测作物氮素胁迫的方法。研究结果表明，田间的不同水、氮制度将影响作物的含氮水平及叶绿素含量，它们之间呈正响应关系。氮素对作物叶绿素含量起着较强的影响作用。作为一种测量叶片叶绿素含量的仪器，叶绿素仪可以作为测量作物含氮的仪器使用，具有快速、准确、简便的特点。     

土壤水分及有机肥料对番茄叶片光合特性及叶绿素含量影响的试验研究

有机栽培是一种新型的种植方式,为了研究有机肥料在不同的水分条件对温室番茄的光合特性以及叶绿素含量的影响,在中国农业大学联栋玻璃温室进行了番茄的盆栽试验研究,采取4种灌水处理以及2种肥料处理,得到如下结论:1随着土壤水分的增加、番茄叶片的净光合速率、蒸腾速率、气孔导度都有相应的增加趋势。2水分最低的处理反而净光合速率的值较高,是由于在这一处理中,叶片的叶绿素含量较高。3土壤含水率较低的前提下(田持的40%～85%),施加有机肥料BGA可以使得番茄叶片的叶绿素含量有较大的增加,平均可增加13%～24%,土壤含水率较高的情况下(田持的85%～100%)叶绿素的含量变化不大。     

盐胁迫对裸果木幼苗光合特性的影响

本研究以一年生裸果木幼苗为材料，采用盆栽育苗方式，设计7个不同浓度NaCl溶液（CK、0.2%、0.4%、0.6%、0.8%、1.0% 和1.2%）模拟盐分胁迫，测定分析了其叶片气体交换参数、光响应曲线及叶绿素荧光参数的变化规律。结果表明：随着盐浓度的增加，叶片净光合速率（P_n）、气孔导度（G_s）和蒸腾速率（T_r）均呈下降趋势，NaCl浓度≥0.4%时，各处理P_n、G_s和T_r均显著低于CK；气孔限制值（L_s）和瞬时水分利用效率（WUE）随盐浓度增加呈先升后降趋势，胞间CO₂浓度（C_i）呈相反趋势，NaCl浓度为0.4%时，L_s达到最大值，而C_i达到最小值，说明P_n下降以气孔限制因素为主，而当NaCl浓度≥0.6%时，以非气孔限制为主要因素。随着盐胁迫程度的增大，最大净光合速率（P_nmax）、暗呼吸速率（R_d）、光饱和点（L_SP）、表观量子效率（A_QY）逐渐降低，光补偿点（L_CP）逐渐增加，表明盐分抑制了幼苗对光的吸收、利用和转换能力。叶片PSII潜在活性（F_v/F₀）、原初光能转化效率（F_v/F_m）、实际光化学效率（Φ_PSII）、电子传递速率（ETR）和光化学猝灭系数（q_p）随着盐浓度的增加呈下降趋势；非光化学猝灭系数（NPQ）在≤0.4%NaCl处理下较CK显著增加，盐浓度≥0.8%时，NPQ显著下降。基质的NaCl浓度在0.2%和0.4%时，裸果木叶片P_n、F_v/F₀、F_v/F_m下降不显著，WUE有所提高，PSII系统可以通过耗散过剩的光能保护光合机构，表现出一定的耐盐性；但盐浓度超过0.6%时，光合生态幅变窄，光合机构受到明显破坏，显著抑制了光合作用能力。     

高温胁迫下银杏离体枝条叶片的叶绿素荧光特性

银杏是一种重要经济树种，随着全球气温升高，高温适应性问题已经影响了其在低纬度区域的栽培。为了探明高温胁迫对银杏叶片光合过程的影响，本研究中离体银杏枝条被置于不同温度（25 ℃、30 ℃、35 ℃、40 ℃、45 ℃）下处理，随即对其叶绿素荧光参数进行监测。 结果显示：40 ℃以上高温胁迫下，银杏叶片的光系统II（PSⅡ）潜在光化学效率（Fv/Fo）、最大光化学效率（Fv/Fm）显著下降；最大荧光（Fm）、最大相对电子传递速率（rETRmax）、PSⅡ实际光化学效率（Yield）、光化学淬灭系数（qP）均显著下降；而初始荧光（Fo）和非光化学淬灭系数（NPQ）在40℃以上高温胁迫下显著上升。本研究表明，银杏离体枝条叶片光合作用对温度变化较敏感，推测高温可能伤害了光合机构，从而对光能的吸收、转换与光合电子传递都有显著的影响。银杏离体枝条叶片的叶绿素荧光动力学参数对高温的敏感性，可能有助于快速筛选和培育耐高温的银杏品种。     

剪叶对槟榔生长状况的影响

为探寻促使槟榔(Areca catechu)叶片早脱落,槟榔提前开花的方法,以七年生海南本地槟榔为试验对象,对槟榔最下面3片大叶进行纵向不同程度剪叶,研究其对叶片含水量、叶绿素含量及内含物的影响,以此了解剪叶对槟榔生长状况的影响.结果表明,剪叶处理对槟榔生长状况产生了一定的影响,剪叶程度不同产生的影响也不同.对照组中即自然状态下叶片含水量一直处于下降趋势,纵向剪叶可使叶片含水量处于比较平稳的状态;剪叶对最下面的叶片叶绿素含量影响明显,剪叶程度越大越明显,但对于整体叶绿素含量无明显影响;剪叶后蛋白质含量先升后降;淀粉含量先降再升再降再升;可溶性糖含量先升高后降低最后保持平衡状态.     

Effect op foliar sprays of sulphuric acid with and without elemental sulphur in the prevention of chlorosis In Peanut (Arachis hypogaea. L.)

Abstract

A field experiment was conducted on an alkaline calcareous soil of Agronomy farm of the College of Agriculture, University of Udaipur to study the effect of sulphur on crop yield, leaf elemental composition, chlorophyll synthesis and activities of haematin enzymes. Application of elemental sulphur 21 days before planting with and without 0.1 per cent foliar sprays of H₂SO₄. increased crop yield by 137 to 197 per cent. Application of all these treatnents had no effect on nitrogen, phosphorus and potassium content of leaves but sulphur content was signficantly increased. Iron content of green leaves was decreased significantly 4 to 5 times together with an increase in the activity of catalase and peroxidase enzymes. Chlorosis in these soils appears to be on account of a reduced physiological availability of iron after absorption.     

Gas Exchange of Five Warm‐Season Grain Legumes and their Susceptibility to Heat Stress

Objective of this study was to compare the heat stress performance of four pulses from dry and hot areas (mungbeans, limabeans, and teparybeans and cowpeas) with that of soybeans. Two experiments were conducted in growth chambers, and data were pooled because results of both experiments were similar. Plants were raised up to flowering at 24/17 °C (day/night) and were then either exposed to these temperatures until maturity or stressed with 33/24 °C for 2 weeks starting at day 1 or 15 after onset of flowering (early vs. late stress). Before, during and after these stress intervals, gas exchange of representative upper leaves was examined; additionally, immediate effects of increasing leaf temperatures from 24 to 32 or 40 °C on chlorophyll fluorescence were assessed. Without heat stress rates of photosynthesis (P_n), and of transpiration (TR), stomatal and mesophyll conductance (g_s, g_m) and intrinsic transpiration efficiency (iTE) differed significantly among the five crops at each date. However, because of crop‐specific time‐courses ranking among unstressed crops was instable with time, so values were integrated or averaged over time. This procedure revealed high P_n potentials in mung‐ and teparybeans and high iTE values in limabeans compared to the other crops. Heat stress lowered P_n and g_s considerably, but increased TR in all five crops. Relative lowering of P_n during heat stress displayed a crop‐specific pattern with limabeans being least susceptible to both early and late heat stress, while cowpeas were highly susceptible to early stress. Effects on P_n were mainly attributable to lowering of g_s and only in part to g_m. The latter was supported by very small changes (<10 %) of various chlorophyll fluorescence signals shortly after raising leaf temperature to 32 °C in all species. However, in limabeans, a decreased electron transport rate (e‐rate, ?18 %) and an increased non‐photochemical quenching (Q_N, +16 %) pointed to an adaptive mechanism to avoid oxidative strains under heat. Leaf temperatures of 40 °C immediately provoked stronger changes in all fluorescence signals than 32 °C; substantial damages at 40 °C were indicated by effective quantum yield, photochemical quenching and ratio of fluorescence decrease in mungbeans and low ones in cowpeas and soybeans. Nevertheless, some adaptive responses of e‐rates and Q_N were observed in all crops and were most expressed in limabeans.     

感染烟草花叶病毒(TMV)的烟叶在显症前的荧光光谱变化

通过测试ＴＭＶ侵染叶片的叶绿素含量与荧光光谱特征的动态变化，分析影响荧光光谱的因素，在以４８０ｎｍ作为激发波长时，叶片６８５ｎｍ与７４０ｎｍ荧光强度之比（Ｆ６８５／Ｆ７４０）随着病毒侵染时间的延长而增加，叶绿素含量则随着病毒侵染时间的延长而降低，因此提出可用Ｆ６８５／Ｆ７４０与叶绿素含量之比作为ＴＭＶ早期诊断的指标。