Influence of chloride and sulphate ions on soil enzymes

Ammonium chloride (AC) and ammonium sulphate (AS) are commonly used nitrogen fertilizers. But the effect of chloride and sulphate ions from these fertilizers on soil enzyme activity has received scant attention. Hence, we conducted a pot culture study to assess the influence of chloride (as AC) and sulphate (as AS) on the activities of urease, amidase, phosphatase and dehydrogenase in soil using rice as the test crop. Chloride and sulphate levels were fixed at 132, 264 and 396 kg ha⁻¹ respectively. Controls were also performed. The enzymes were assayed at three stages of the crop growth viz., active tillering, panicle initiation and harvest.
The enzyme activities decreased with increasing chloride and sulphate levels; however, the degree of inhibition varied among the enzymes assayed and the nature and amounts of salts added. The inhibition may be due to the specific effects of chloride and sulphate ions on microbial growth and subsequent enzyme synthesis, osmotic desiccation leading to microbial cell lysis, and a salting-out effect modifying the ionic conformation of the active site of the enzyme protein.     

观赏植物诱变育种的研究现状和展望

本文综述了观赏植物辐射诱变育种的研究现状,并对花卉辐照诱变的手段、方法、处理材料和适宜辐照剂量、辐照机理与方法及应用作了总结,同时对观赏植物辐射诱变育种存在的问题及发展方向进行了探讨。     

基施磷肥对石灰性土壤上番茄产量的影响

A lysimeter experiment with undisturbed soil profiles was carried out to study nitrogen cycling and losses in a paddy soll with applications of coated urea and urea under a rice-wheat rotation system in the Taihu Lake region from 2001 to 2003. Treatments for rice and wheat included urea at conventional, 300 （rice） and 250 （wheat） kg N ha^-1, and reduced levels, 150 （rice） and 125 （wheat） kg N ha^-1, coated urea at two levels, 100 （rice） and 75 （wheat） kg N ha^-1, and 150 （rice） and 125 （wheat） kg N ha^-1, and a control with no nitrogen arranged in a completely randomized design. The results under two rice-wheat rotations showed that N losses through both NH3 volatilization and runoff in the coated urea treatments were much lower than those in the urea treatments. In the urea treatments N runoff losses were significantly （P 〈 0.001） positively correlated （r = 0.851） with applied N. N concentration in surface water increased rapidly to maximum two days after urea application and then decreased quickly. However, if there was no heavy rain within five days of fertilizer application, the likelihood of N loss by runoff was not high. As the treatments showed little difference in N loss via percolation, nitrate N in the groundwater of the paddy fields was not directly related to N leaching. The total yield of the two rice-wheat rotations in the treatment of coated urea at 50% conventional level was higher than that in the treatment of urea at the conventional level. Thus, coated urea was more favorable to rice production and environmental protection than urea.     

设施土壤盐分的累积、迁移及离子组成变化特征

通过对我国不同地区设施栽培现状的野外调查和取样分析,研究了设施栽培条件下土壤盐分的累积、迁移及离子组成变化特点。结果表明:1)设施栽培条件下,土壤含盐量的变化幅度大,且均明显高于露地土壤,各研究区域内已有40%8～9%的土壤含盐量超过了作物正常生长的临界浓度。2)设施栽培的可持续利用周期较短,连续种植到4年左右的设施土壤,其耕层盐分的累积量可达到作物的生长障碍临界点,之后因设施使用率的下降以及采取的措施而有所降低,但仍高于露地土壤。设施连续使用会导致土壤环境质量的不断恶化。3)设施土壤剖面(0100.cm)盐分含量均高于相邻露地,盐分含量随土层深度增加而降低,其中耕层(020.cm)的盐分含量显著高于其下各层;盐分离子在土壤剖面的运移同时存在着明显的向底层迁移和向表层聚集两种方式,但以表聚为主;此外,盐分离子的大量累积和向底层迁移,特别是NO3-的淋溶已严重影响到部分地区的地下水水质。4)设施栽培后,土壤中的NO3-、SO42-、Cl-、Ca2+、Mg2+、K+、Na+均有不同程度的累积,阴离子以NO3-和SO42-为主,阳离子以Ca2+为主。盐分的大量累积以及某些离子的相对富集在一定程度上引起了作物养分的供需失衡、土壤酸化、棚室内CO2供应不足等生产问题。     

典型双季稻田基施碳酸氢铵和尿素的氨挥发损失研究

采用密闭室连续抽气法研究了湖南典型双季稻田,尿素和碳酸氢铵基施后的氨挥发特征。结果表明,基施碳酸氢铵(NC)稻田初始氨挥发强度和氨挥发总量大于基施尿素(UR)稻田。早稻季NC处理稻田氨挥发排放量为45.19 kg·hm-2,损失率达30.12%,UR处理氨挥发排放量为32.93 kg·hm-2,损失率达21.95%;晚稻季NC处理稻田氨挥发排放量为70.91 kg·hm-2,损失率达31.93%,UR处理氨挥发排放量为61.78 kg·hm-2,损失率达27.04%。基施尿素能够显著降低稻田氨挥发排放,减少氮素损失。     

灌溉水盐度对滴灌棉田土壤氨挥发的影响

【目的】氨挥发是农田氮素损失的重要途径之一,咸水灌溉直接或间接影响土壤的理化性质,进而影响土壤氨挥发,但目前对于咸水灌溉下氨挥发的报道还较少。因此通过田间试验研究尿素滴灌施肥条件下,淡水和咸水灌溉对棉田土壤氨挥发的影响。【方法】试验设置淡水和咸水两种灌溉水,其电导率(EC)分别为0.35和8.04d S/m(分别用CK和SW表示),氮肥(N)用量为240 kg/hm2。氨挥发的收集采用密闭室法,用稀硫酸作为氨的吸收液,测定用靛酚蓝比色法。【结果】1)灌溉施肥后,咸水滴灌棉田土壤盐分、脲酶活性和铵态氮含量均显著高于淡水滴灌。SW处理土壤电导率(EC1∶5)较CK平均高出4.53倍。灌溉施肥后SW处理土壤脲酶活性迅速增加,第4天达到最大,随后降低,SW处理脲酶活性较CK处理平均增加了20.6%。SW处理土壤铵态氮含量明显高于CK处理,尤其是灌溉施肥后第2天,SW处理铵态氮含量比CK处理增加了66.1%。2)SW处理棉田土壤p H值低于CK处理,但在灌溉施肥周期内都呈先增加后降低趋势,p H的变化在7.6~8.0之间。3)SW处理抑制了硝化作用,SW处理土壤硝态氮含量较CK处理显著降低。SW处理土壤硝态氮含量平均较CK低7.68%。4)3个灌溉施肥周期的平均温度分别为24.6℃、26.05℃和24.9℃,因此在第2个和第3个灌溉施肥周期氨挥发高,第1个灌溉施肥周期的总降水量最大,分别比第2和3个灌溉施肥周期高3.7 mm和10.2 mm,但降水量远远小于灌溉量,因此对于氨挥发影响不大。5)总体上,土壤氨挥发损失量在灌溉施肥后1~2天最大,占氨挥发总量的45.7%~79.3%,随后呈降低趋势;灌溉施肥后第1天土壤氨挥发最大,在3个灌溉施肥周期,SW处理第1天的氨挥发较CK分别增加70.7%、69.43%和60.8%。SW处理棉田土壤氨挥发显著高于CK处理。在三个连续灌溉施肥周期内,SW处理棉田土壤氨挥发累积总量为10.98 kg/hm2,CK处理为7.57 kg/hm2,SW处理较CK处理增加了45.1%。【结论】咸水灌溉促进了脲酶活性,但抑制了土壤的硝化作用,导致铵态氮含量增加,加剧了氨的挥发。温度升高促进土壤氨挥发,少量降雨对氨挥发影响不大。因此,滴灌施肥条件下,咸水灌溉会增加氨挥发损失。     

辐射敏化剂对离子注入小麦生物学效应的影响

采用中国科学院兰州近代物理研究所 2 0 0kV离子注入机产生的不同剂量Fe1+ ,对春小麦新品系 81 52 9种子进行注入并用辐射敏化剂后处理 ,研究其注入效应。结果表明 :①离子注入随注入剂量的增加染色体畸变率呈上升趋势 ;②不同辐射敏化剂后处理对萌发力、染色体畸变率影响程度不同 ;③不同辐射敏化剂对同一剂量Fe1+ 离子注入小麦的染色体畸变率影响的总趋势 :EDTA >秋水仙素 >咖啡因。     

Ion Transport Dynamics in Acid Variable Charge Subsoils

This is a mini-review of the research work conducted by the authors with the objective of studying ion transport in variable charge subsoils collected from different areas around the world. An attempt is made in these studies to relate the unique behavior manifested during ionic transport in these subsoils with their mineralogical, physical and chemical properties, which are markedly different from those in soils from temperate regions. The variable charge subsoils have a relatively high salt sorption capacity and anion exchange capacity (AEC) that retards anions downward movement. The AEC correlates closely with the anion retardation coefficients. Ca²⁺ applied with gypsum in topsoil may be transported to the subsoil and may improve the subsoil chemical properties. These results may help in developing appropriate management strategies under a range of mineralogical, physical, and chemical conditions.     

The effects of nitrogen sludge load and DO on high concentration nitrous nitrifying system

The purpose of this paper is to investigate the impact of nitrogen sludge load and dissolved oxygen(DO) on nitrite nitrification system. The following conclusions have been drawn based on the experimental data and analysis results: 1) The ammonium degradation rate and the nitrous accumulation rate of high concentration nitrous nitrifying system decline with the increase of NH+4-N sludge load. When HRT is less than two days, the ammonium degradation rate rapidly declines to 25%~29% with the increase of NH+4-N sludge load. When HRT is beyond two days, the ammonium rate of the system slowly falls to 50%~60% with the NH+4-N sludge load increased. When HRT is 2.5 days or 3 days, the declined trend of the system nitrous accumulation rate is not obvious with the NH+4-N sludge load increased. When HRT is 5 days, the decrease of nitrous accumulation rate is caused by the adaptation of sludge. So the NH+4-N sludge load of high concentration nitrous accumulation system should not be too high; 2)With the increase of DO, the ammonium degradation of high concentration nitrous nitrifying system is gradually increased. When the DO is less than 0.7 mg/L, it is not conductive to the ammonium degradation. When the DO is higher than 2 mg/L, the oxide of NH+4-N is changed into the increased of NO3-N and the nitrous accumulation rate declined. If the concentration of DO is controlled within 0.7to 1.3 mg/L, the high concentration nitrous nitrifying system can have a good NH+4-N degradation rate and nitrous accumulation rate.